Results 101 to 110 of about 6,381,275 (336)

Mass spectrometry based identification of AMP‐O‐Tris generated by Thermococcus onnurineus Cas10

FEBS Open Bio, EarlyView.
Isolated Thermococcus onnurineus Cas10 generates the noncanonical ATP‐derived product AMP‐O‐Tris while in Tris‐containing buffer as identified via mass spectrometry, revealing relaxed nucleophile selectivity under isolated conditions. These findings suggest that multiprotein Csm complex assembly restricts Cas10 reactivity toward canonical cyclic ...
Su‐Jin Lee, Ga Seul Lee, JinHan Kim, Kwang‐Hyun Park, Seong‐Ryung Go, Jeong Hee Moon, Eui‐Jeon Woo   +6 more
wiley   +1 more source

Molecular dynamics simulations of positively selected codons in FcγRI reveal novel biochemical binding properties

FEBS Open Bio, EarlyView.
Evolutionary analysis across 32 placental mammals identified positive selection at residues H148 and W149 in the immune receptor FcγR1. Ancestral reconstruction combined with molecular dynamics simulations reveals how these mutations may influence receptor structure and dynamics, providing insight into the evolution of antibody recognition and immune ...
David A. Young, Omar Arias‐Gaguancela, Fiona Gibson, Morgan Grainger, Jodie Score, Amit Nathubhai, Mauricio Cafiero, Lee Richard Machado   +7 more
wiley   +1 more source

Structural model updating and prediction variability using Pareto optimal models [PDF]

, 2008
A multi-objective identification method for structural model updating based on modal residuals is presented. The method results in multiple Pareto optimal structural models that are consistent with the experimentally measured modal data and the modal ...
Konstantinos Christodoulou (7175594), Panetsos, Panagiotis, Ntotsios, E., Evangelos Ntotsios (7175591), Papadimitriou, Costas, Costas Papadimitriou (99228), Panetsos, P., Christodoulou, Konstantinos, Ntotsios, Evaggelos, Christodoulou, K., Papadimitriou, C., Panagiotis Panetsos (7176440)   +11 more
core   +1 more source

Structural Flexibility of the Monomeric Red Fluorescent Protein DsRed

Crystals
The monomeric red fluorescent protein DsRed (mDsRed) is widely used as an optical probe for multicolor applications in flow cytometry or fluorescence microscopy.
Ki Hyun Nam
doaj   +1 more source

Versatile vector tools for efficient protein screening across multiple expression systems

FEBS Open Bio, EarlyView.
A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu, Yaqing Liu, Lei Qin, Feng Yu, Qisheng Wang, Yahui Liu   +5 more
wiley   +1 more source

UiO‐66 metal–organic frameworks in biomedicine: From structural tunability to bioimaging, photodiagnostics, and photodynamic cancer therapy

FEBS Open Bio, EarlyView.
UiO‐66(Zr) metal–organic frameworks are chemically stable, biocompatible, and highly tunable nanomaterials. Their modular structure enables controlled drug delivery, multimodal bioimaging, and light‐activated photodynamic therapy, supporting integrated diagnostic and therapeutic (theranostic) applications in cancer and biomedical research.
Veronika Huntošová, Grigorii Rakhalskii, Miroslav Almáši   +2 more
wiley   +1 more source

Frustrated Flexibility in Metal-Organic Frameworks

, 2020
Stimuli-responsive flexible metal-organic frameworks (MOFs) remain at the forefront of porous materials research due to their enormous potential for various technological applications.
Rochus, Schmid, Kai, Terlinden, Julian, Keupp, Julia, Kotschy, Sebastian, Henke, Andrea, Machalica, Michael T., Wharmby, Suresh K., Vasa, Marvin, Kloß, Philip A., Chater, Rasmus, Linser, Louis, Frentzel-Beyme, Christian, Sternemann, Roman, Pallach   +13 more
core   +1 more source

Hinge Atlas: relating protein sequence to sites of structural flexibility

BMC Bioinformatics, 2007
Background Relating features of protein sequences to structural hinges is important for identifying domain boundaries, understanding structure-function relationships, and designing flexibility into proteins.
Yang Julie, Lu Long J, Flores Samuel C, Carriero Nicholas, Gerstein Mark B   +4 more
doaj   +1 more source

The crystal structure of the Borrelia burgdorferi nicotinamidase BBE22 resolves a long‐standing annotation error

FEBS Open Bio, EarlyView.
The crystal structure of Borrelia burgdorferi nicotinamidase (PncA/BBE22) reveals the correct full‐length protein initiated from a non‐canonical AUU start codon. The structure validates previous biochemical findings and resolves a long‐standing annotation error, demonstrating that the truncated database sequence is structurally incompatible with the ...
Kalvis Brangulis
wiley   +1 more source

LABOUR MARKET FLEXIBILITY IN ESTONIA: WHAT MORE CAN BE DONE? [PDF]

In mid-2008, high employment and low unemployment rates characterised the Estonian labour market in comparison with the average of the EU15 countries. While aggregate outcomes improved during 2000 07, large inequalities persisted across regions, ethnic ...
Zuzana Brixiova
core