Results 141 to 150 of about 395,744 (213)

Directed evolution of enzymes at the crossroads of tradition and innovation

FEBS Open Bio, EarlyView.
An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova, Andrej Mirossay, Erik Sedlak   +2 more
wiley   +1 more source

Complex de novo structural variants are an underestimated cause of rare disorders. [PDF]

Nat Commun
Jung H, Yang TP, Walker S, Danecek P, Garcia-Salinas OI, Neville MDC, Christopher J, Cortés-Ciriano I, Firth H, Scally A, Hurles M, Campbell P, Rahbari R.   +12 more
europepmc   +1 more source

Hyperactive ice‐binding proteins stabilize cell membranes and improve resistance to dehydration stress in Caenorhabditis elegans

FEBS Open Bio, EarlyView.
TisIBP8, a fungal‐derived hyperactive ice‐binding protein, helps Caenorhabditis elegans survive dehydration. It localizes near cell membranes, reduces cell damage, and helps maintain membrane structure during drying. These results suggest that ice‐binding proteins can protect cells from dehydration stress as well as freezing stress.
Daiki Shimose, Kotaro Ozaki, Ryohei Kuriyama, Yuka Ikemoto, Kazuhiro Mio, Yuji C. Sasaki, Tatsuya Arai, Sakae Tsuda, Yoichi Shinkai, Masahiro Kuramochi   +9 more
wiley   +1 more source

Surfactin Structural Variants Differentially Modulate Plant Immune Responses. [PDF]

Biomolecules
Ding N, Dong H, Thomas R, Gilliard G, Pršić J, Ongena M.   +5 more
europepmc   +1 more source

Molecular characterization of covRS mutations in M1UK Streptococcus pyogenes

FEBS Open Bio, EarlyView.
Group A Streptococcus (GAS) acquires covRS mutations driving a hypervirulent bacterial state, frequently associated with invasive disease‐like necrotizing fasciitis. We demonstrate that the newly emerged M1UK GAS lineage can also acquire these mutations.
Jarrad Pritchard, Emma‐Jayne Proctor, Jiawa Wang, Nikolas P. Johnston, Andrew Hayes, Georgia McCorkell, Haibo Yu, Jai Tree, Mark R. Davies, Mark J. Walker, Ronald Sluyter, Stephan Brouwer, Martina L. Sanderson‐Smith   +12 more
wiley   +1 more source

Screening of Hidden Pathogenic Structural Variants in PRKN. [PDF]

Mov Disord
Daida K, Cogan G, Tesson C, Lesage S, Schaake S, Balck A, Trinh J, Lohmann K, Malik L, Baker B, Paquette K, Moller A, Sue C, Funayama M, Hattori N, Singleton A, Klein C, Brice A, Billingsley KJ, Blauwendraat C.   +19 more
europepmc   +1 more source

Hyperosmotic stress‐induced redistribution of pre‐mRNA cleavage factor I subunits is associated with shifts in alternative polyadenylation

FEBS Open Bio, EarlyView.
Hyperosmotic stress triggers the relocation of the CFIm complex from the nucleus to the cytoplasm. This shift creates a nuclear ‘stoichiometric bottleneck’, limiting CFIm availability for mRNA processing. Consequently, specific mRNAs like NUDT21 and DICER1 undergo targeted 3′UTR shortening, demonstrating how spatial protein dynamics drive rapid ...
Hitomi Soumiya, Masatake Osawa, Hidefumi Fukumitsu   +2 more
wiley   +1 more source

Multiplatform comparisons and annotation of structural variants highlight the utility of the T2T reference genome in human diagnostics. [PDF]

Gigascience
Savara J, Novosad T, Gajdos P, Petrackova A, Behalek M, Manakova J, Ctvrtlik F, Minarik J, Papajik T, Kriegova E.   +9 more
europepmc   +1 more source

Effects of IGFBP4 deficiency on human preadipocyte proliferation and differentiation through the IGF1R/AKT pathway

FEBS Open Bio, EarlyView.
IGFBP4 knockdown (KD) impairs preadipocyte proliferation and is associated with IGF1R protein downregulation and attenuated AKT phosphorylation. The mechanisms by which IGFBP4 KD influences the IGF1R/AKT signaling pathway involve newly synthesized proteins and lysosomal degradation pathways. Created in BioRender.
Yujia Guo, Sandy Richter, Christoph Bach, Wieland Kiess, Anna Kirstein, Diana Le Duc, Antje Garten   +6 more
wiley   +1 more source

Sub-pangenome analysis reveals structural variants associated with fruit color and bacterial wilt resistance in eggplant. [PDF]

Nat Commun
You Q, Peng Z, Li Z, Jiang Y, Wan P, Zhao Y, Zhao W, Zhang S, Cheng H, Chen C, Heng Z, Hu M, Zhou Y, Gaut BS, Sun B, Li T, Liao Y.   +16 more
europepmc   +1 more source