Results 131 to 140 of about 5,333,553 (268)

Mixed‐class J‐domain protein scaffolds promote expanded aggregate handling and multivalent Hsp70 engagement during functional disaggregase assembly

FEBS Letters, EarlyView.
Protein aggregates threaten proteostasis and cell health. In human cells, Hsp70–J‐domain protein‐based disaggregases remove aggregates, but how they assemble remains unclear. Our biochemical findings show that DNAJA2‐ and DNAJB1‐containing disaggregase scaffolds enhance luciferase aggregate targeting, and that Hsp70 recruitment by both J‐domain ...
Anna Szlachcic, Nadinath B. Nillegoda
wiley   +1 more source

Pain and Nutrition in Dementia and Alzheimer's Phase 1: a cross-sectional, observational study design. [PDF]

Front Dement
Judkins TC, Andrews HJ, Song Q, Clark EI, Tate C, Wais JI, Fillingim R, Huo Z, DeKosky ST, Gower B, Cohen RA, Ebner NC, Cruz-Almeida Y, Strath LJ.   +13 more
europepmc   +1 more source

Reconstructing enzyme evolution by protein engineering

FEBS Letters, EarlyView.
Natural enzyme evolution can be retraced by protein engineering methods such as directed evolution, rational design, and ancestral sequence reconstruction. These approaches reveal how enzymes emerged from ligand‐binding scaffolds, developed varying substrate preferences, formed oligomeric complexes, adapted to environmental changes, and evolved novel ...
Lukas Drexler, Markus R. Busch, Reinhard Sterner   +2 more
wiley   +1 more source

The Danish National Survey of Diet and Physical Activity (DANSDA) 2021-2024: Study Design and Participants Characteristics. [PDF]

Nutrients
Christensen C, Biltoft-Jensen AP, Matthiessen J, Henriksen K, Sørensen MR, Christensen T, Trolle E, Fagt S.   +7 more
europepmc   +1 more source

A study design to identify associations [PDF]

BJOG: An International Journal of Obstetrics & Gynaecology, 2018
Alexander, Melamed, Julian N, Robinson
openaire   +2 more sources

Decoding the dynamic extracellular matrix in cancer—3D models and bioscaffolds rewire the rules of tumor progression

FEBS Letters, EarlyView.
Cancer progression is regulated by the dynamic matrix code of the tumor microenvironment, which influences cellular behavior and disease development. Importantly, matrix remodeling in three‐dimensional cancer models more accurately reflects in vivo conditions compared to conventional two‐dimensional systems.
Sylvia Mangani, Konstantinos Spanopoulos, Zoi Piperigkou, Nikos K. Karamanos   +3 more
wiley   +1 more source

The Canadian multi-ethnic research on aging (CAMERA) study: Design, participant characteristics, and preliminary findings. [PDF]

Alzheimers Dement
Marawi T, Rai H, Kumar R, Vandeloo KL, Yep R, Alexander MW, Boshmaf SZ, Chen SM, Gopinath G, Malhotra S, Zhang A, Nyman AJ, Perri LX, Sit V, Splinter TFL, Munoz DP, Swardfager W, Ryan JD, Black SE, Goubran M, Rabin JS.   +20 more
europepmc   +1 more source

Identification of a Shiga toxin A‐derived peptide internalized into Gb3 receptor‐bearing cells via interaction with the Shiga toxin B subunit

FEBS Letters, EarlyView.
The process of internalization of the Shiga toxin A subunit via formation of a complex with the Shiga toxin B subunit, which specifically binds to the Gb3 receptor. The peptide is designed to act as a carrier of drugs into cancer cells. Here, we explored the potential of peptides derived from the catalytic A subunit of Shiga toxin (STxA) to be drug ...
Giulia Opassi, João C. Encarnação, Valeria Napolitano, Grzegorz Dubin, Grzegorz Popowicz, Karl Andersson, U. Helena Danielson   +6 more
wiley   +1 more source

Proposed study design for estimating COVID-19 vaccine effectiveness in post-pandemic Taiwan. [PDF]

J Chin Med Assoc
Chen LS, Lee CY, Kuo HW, Liu YL, Chuang JH.   +4 more
europepmc   +1 more source

Investigating transcription factor dynamics in health and disease using FRAP

FEBS Letters, EarlyView.
FRAP analysis of GFP‐tagged transcription factors reveals how molecular mobility and target engagement change in response to drug treatment. By combining live‐cell imaging, quantitative model fitting, and statistical analysis, this approach uncovers transcription factor dynamics linked to disease mechanisms, providing a powerful framework for ...
Kannan Govindaraj, Carolina Vazquez Garzon, Jose Joaquin Velasco, Janine N. Post   +3 more
wiley   +1 more source