Prevalence, Antimicrobial Resistance, and Resistance Gene Profiles of Extended-Spectrum β-Lactamase-Producing Escherichia coli and Klebsiella pneumoniae Isolated From Quails in Sylhet, Bangladesh. [PDF]
MicrobiologyopenThis study investigated the molecular epidemiology and antimicrobial resistance of Escherichia coli and Klebsiella pneumoniae isolated from quails in Bangladesh. High prevalence of E. coli, including Shiga toxin‐producing strains, and widespread multidrug resistance were observed.
Mamun MA +10 more
europepmc +2 more sources
Amplification-free, highly sensitive electrochemical DNA-based sensor for simultaneous detection of stx1 and stx2 genes of Shiga toxin-producing E. coli (STEC) [PDF]
, 2022 Shiga toxin-producing E. coli (STEC) is a food-borne pathogen of significant public health concern, due to the severity of the illness it can cause and a low infection dose. The key targets in molecular-based assays to detect STEC are stx1 or stx2 genes,
Sofia Rodrigues , Teixeira +5 more
core +2 more sources
Scientific Reports, 2023 Shiga toxin (Stx) is the key virulence factor of Stx-producing Escherichia coli (STEC). All known Stxs (Stx1 and Stx2) are encoded by bacteriophages (Stx phages).
Bungo Yano +4 more
doaj +1 more source
Escherichia coli0157:H7 Strains That Express Shiga Toxin (Stx) 2 Alone Are More Neurotropic for Gnotobiotic Piglets Than Are Isotypes Producing Only Stx1 or Both Stx1 and Stx2 [PDF]
The Journal of Infectious Diseases, 2000 Infection with Escherichia coli O157:H7 can lead to hemolytic uremic syndrome (HUS) in some children. Epidemiologic data suggest that Shiga toxin (Stx) 2-producing strains are more frequently associated with HUS than are Stx1-producing strains. Less clear is whether strains that express Stx2 alone are more frequently associated with HUS than strains ...
Arthur Donohue‐Rolfe +4 more
openaire +1 more source
Phylogenetic analysis of Shiga toxin 1 and Shiga toxin 2 genes associated with disease outbreaks
BMC Microbiology, 2007 Background Shiga toxins 1 and 2 (Stx1 and Stx2) are bacteriophage-encoded proteins that have been associated with hemorrhagic colitis, hemolytic uremic syndrome and other severe disease conditions.
Farrell Larry D +5 more
doaj +1 more source
Neutralization of Stx1 toxicity by Stx1 mAbs.
, 2014 A. Administration of mAbs Stx1-1, Stx1-2, or Stx1-3 at 1, 10, or 100 µg/mL fully protects Vero cells from Stx1 toxicity (partially pure toxin at 1 ng/mL). Photographs are of Vero cells treated with 1 ng/mL Stx1 and 100 µg/mL mAb. B.
Xiaohua He (361530) +4 more
core +1 more source
Stx1 ELISA sensitivity and subtype specificity.
, 2014 A. Standard curve for an anti-Stx1 sandwich ELISA using mAbs Stx1-2 and Stx1-1. Purified Stx1 (E167Q) toxoid is used as the antigen; mAb Stx1-2 as the capture antibody (at 1 µg/mL), and mAb Stx1-1-biotin as the detection antibody (at 0.25 µg/mL).
Xiaohua He (361530) +4 more
core +1 more source
Journal of Advanced Veterinary and Animal Research, 2016 Objectives: This study was designed to assess the contamination of enterovirulent Escherichia coli with table eggs at Mansoura, Egypt. Materials and methods: A total of 100 commercially available table eggs were randomly collected from various ...
Mahmoud Elafify +3 more
doaj +1 more source
Detection of hybrid Stx1/Stx2.
, 2014 A. Using a Stx1 capture antibody and a Stx2 detection antibody, hybrid Stx1/2 molecules were detected by ELISA. Cell-free medium from mitomycin-induced STEC strains containing Stx1, Stx2, both Stx1 and Stx2, or neither was applied to the ELISA at 1/20 ...
Xiaohua He (361530) +4 more
core +1 more source
Blockade of the SNARE protein syntaxin 1 inhibits glioblastoma tumor growth. [PDF]
PLoS ONE, 2015 Glioblastoma (GBM) is the most prevalent adult brain tumor, with virtually no cure, and with a median overall survival of 15 months from diagnosis despite of the treatment. SNARE proteins mediate membrane fusion events in cells and are essential for many
Fausto Ulloa +9 more
doaj +1 more source