Construction and use of anstx1transcriptional fusion togfp [PDF]
FEMS Microbiology Letters, 2005 Shiga toxins (Stxs), also termed Vero toxins, are cytotoxic ribosome inactivating proteins that are produced by a number of gastrointestinal pathogens and that contribute to the severity of the associated diseases. In this work, we constructed and validated a transcriptional fusion of the stx1AB promoter to the gfp reporter gene.
Abram, Aertsen +2 more
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Induction of cytokines in a human colon epithelial cell line by Shiga toxin 1 (Stx1) and Stx2 but not by non‐toxic mutant Stx1 which lacks N‐glycosidase activity [PDF]
FEBS Letters, 1999 Stx1 and Stx2 produced by Shiga toxin‐producing Escherichia coli are cytotoxic due to their N‐glycosidase activity on 28S rRNA. In this study, we have shown that proinflammatory cytokine mRNAs, especially IL‐8, were induced by Stx1 and Stx2 in Caco‐2 cells. A non‐toxic mutant of Stx1 which lacks N‐glycosidase activity did not induce cytokine mRNAs.
Yamasaki, Chisato +6 more
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Virulence and extended-spectrum β-lactamase encoding genes in Escherichia coli recovered from chicken meat intended for hospitalized human consumption [PDF]
Veterinary World, 2017 Aim: This study describes the prevalence of Escherichia coli in frozen chicken meat intended for human consumption with emphasis on their virulence determinants through detection of the virulence genes and recognition of the extended-spectrum β-lactamase
Gamal A. Younis +3 more
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Prevention of Shiga toxin 1-caused colon injury by plant-derived recombinant IgA
Scientific Reports, 2022 Immunoglobulin A (IgA) is a candidate antibody for oral passive immunization against mucosal pathogens like Shiga toxin-producing Escherichia coli (STEC).
Katsuhiro Nakanishi +14 more
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Characterization and zoonotic impact of Shiga toxin producing Escherichia coli in some wild bird species [PDF]
Veterinary World, 2017 Aim: Wild birds are considered silent vectors of some zoonotic water and food borne pathogens of public health significance. Owing to the importance of Shiga toxin producing Escherichia coli (STEC) as the most pathogenic among the emerging diarrheagenic ...
Hanaa Mohamed Fadel +2 more
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Journal of Applied Veterinary Sciences, 2022 Escherichia coli causes many health problems in lambs, the most prominent of which is diarrhea, the study aimed to detect the shiga toxins genes in E.coli isolated from the suckling lambs affected with diarrhea in Nineveh governorate. 91 fecal samples were collected from suckling lambs from different areas of Nineveh governorate during February 2022 ...
A.H. Al-Sabawi, Dh.M. Jwher
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Detection of Shiga toxin produced by Escherichia coli in poultry and meat in Luxor city using multiplex PCR [PDF]
Benha Veterinary Medical Journal, 2016 Shiga toxins were widely spread in the meat especially in minced meat. These toxins have an important significance tohuman health because it is a major cause of food poisoning.
Reham Ahmed +3 more
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Reexamination of N-terminal domains of syntaxin-1 in vesicle fusion from central murine synapses
eLife, 2021 Syntaxin-1 (STX1) and Munc18-1 are two requisite components of synaptic vesicular release machinery, so much so synaptic transmission cannot proceed in their absence. They form a tight complex through two major binding modes: through STX1’s N-peptide and
Gülçin Vardar +7 more
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Detection of shiga toxin strains of Escherichia coli non O157 in different soft cheese by polymerase chain reaction [PDF]
Benha Veterinary Medical Journal, 2019 The objectives of the current study were detection and characterization of the isolated Escherichia coli non O157 using PCR assay. A total of 90 cheese samples high salt soft cheese, Kareish cheese and Tallaga cheese were examined for E.
Nahed Wahba +4 more
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Glycolipid binding preferences of Shiga toxin variants. [PDF]
PLoS ONE, 2014 The major virulence factor of Shiga toxin producing E. coli, is Shiga toxin (Stx), an AB5 toxin that consists of a ribosomal RNA-cleaving A-subunit surrounded by a pentamer of receptor-binding B subunits.
Sayali S Karve, Alison A Weiss
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