Results 161 to 170 of about 2,216,866 (310)

Directed evolution of enzymes at the crossroads of tradition and innovation

FEBS Open Bio, EarlyView.
An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova, Andrej Mirossay, Erik Sedlak   +2 more
wiley   +1 more source

Hyperactive ice‐binding proteins stabilize cell membranes and improve resistance to dehydration stress in Caenorhabditis elegans

FEBS Open Bio, EarlyView.
TisIBP8, a fungal‐derived hyperactive ice‐binding protein, helps Caenorhabditis elegans survive dehydration. It localizes near cell membranes, reduces cell damage, and helps maintain membrane structure during drying. These results suggest that ice‐binding proteins can protect cells from dehydration stress as well as freezing stress.
Daiki Shimose, Kotaro Ozaki, Ryohei Kuriyama, Yuka Ikemoto, Kazuhiro Mio, Yuji C. Sasaki, Tatsuya Arai, Sakae Tsuda, Yoichi Shinkai, Masahiro Kuramochi   +9 more
wiley   +1 more source

Enzyme Stereoselectivity and Substrate Specificity of Aldo-KetoReductases

, 2015
Enzyme Stereoselectivity and Substrate Specificity of Aldo ...
Flingos, Emily, Smith, Brittany, Rivas-Torres, Esmeralda, Edwards, Julianna, Rothbarth, Sierra   +4 more
core  

Machine learning-guided dual optimization of the substrate specificity and thermostability of yeast alcohol dehydrogenase. [PDF]

Biotechnol Biofuels Bioprod
Ogawa Y, Kondo A, Shirai T.
europepmc   +1 more source

From energy provision to protein synthesis: Tunnelling nanotubes as mediators of intercellular metabolic cooperation in cancer

FEBS Open Bio, EarlyView.
The cytoskeleton‐mediated transport of mitochondria via tunnelling nanotubes restores respiration, increases ATP production, rescues cells from apoptosis, activates the AKT/mTOR signalling pathway, promotes cell migration and invasiveness, contributes to cancer progression and treatment resistance.
Stanislava Martínková, Jan Trnka
wiley   +1 more source

Understanding Enzyme Stereoselectivity and Substrate Specificity of Aldo-Keto Reductases

, 2015
Understanding Enzyme Stereoselectivity and Substrate Specificity of Aldo-Keto ...
Flingos, Emily, Smith, Brittany, Rivas-Torres, Esmeralda, Edwards, Julianna, Rothbarth, Sierra   +4 more
core  

Substrate Specificity of B12-Depedent Ribonucleotide Reductases: Biotechnology and Metabolic Implications. [PDF]

Chembiochem
Eltoukhy L, Loderer C.
europepmc   +1 more source

Yeast Gcn2 retains activity following humanization of its auto‐phosphorylation region

FEBS Open Bio, EarlyView.
Using Saccharomyces cerevisiae as a model to study Gcn2 activation and regulation is limited by the lack of antibodies detecting phosphorylated Gcn2. To overcome this, we engineered Gcn2‐HsC, a yeast Gcn2 variant recognizable by commercial anti‐human phospho‐GCN2 antibodies.
Reuben A. Anderson, Anja H. Schiemann, Evelyn Sattlegger   +2 more
wiley   +1 more source

Mapping the Substrate Specificity Landscape of PAD2 and PAD4 Enzymes. [PDF]

Chembiochem
Borbély A, Steckel A, Papp D, Pál D, Uray K, Gőz VG, Schlosser G.   +6 more
europepmc   +1 more source

MagmaFlow: A desktop platform for artificial intelligence‐driven expression analysis

FEBS Open Bio, EarlyView.
MagmaFlow is a free, no‐code platform for gene expression analysis. It generates interactive volcano plots, links genes to literature, pathways, and diseases, prioritizes candidates using millions of publications, identifies affected biological processes, builds network diagrams, and exports publication‐ready figures and reports for macOS and Windows ...
Carlos E. Buss, Ao Li, Eduardo H. Gilglioni, Mayank Bansal, Sumeet Pal Singh, Latifa Bakiri, Alessandra K. Cardozo, Esteban N. Gurzov   +7 more
wiley   +1 more source