Results 61 to 70 of about 453,916 (245)

Block-based characterization of protease specificity from substrate sequence profile

BMC Bioinformatics, 2017
Background The mechanism of action of proteases has been widely studied based on substrate specificity. Prior research has been focused on the amino acids at a single amino acid site, but rarely on combinations of amino acids around the cleavage bond ...
Enfeng Qi, Dongyu Wang, Bo Gao, Yang Li, Guojun Li   +4 more
doaj   +1 more source

Structural basis for substrate specificity and catalysis of α1,6-fucosyltransferase

Nature Communications, 2020
Core-fucosylation of the N-glycan core is an essential biological modification and the α1,6- fucosyltransferase FUT8 is the only enzyme in humans that catalyses this modification through the addition of an α-1,6-linked fucose to N-glycans.
Ana García-García, Laura Ceballos-Laita, Sonia Serna, Raik Artschwager, Niels C. Reichardt, Francisco Corzana, Ramon Hurtado-Guerrero   +6 more
doaj   +1 more source

Substrate specificity of bleomycin hydrolase

Biochemical Pharmacology, 1989
Bleomycin (BLM) hydrolase is believed to protect both malignant and normal tissue from the toxicity of the antitumor drug BLM. Little is known about the substrate specificity of BLM hydrolase. Thus, we developed ion-paired reverse phase high speed liquid chromatography systems to assay for the metabolism of several BLM analogs.
S M, Sebti, J C, DeLeon, L T, Ma, S M, Hecht, J S, Lazo   +4 more
openaire   +2 more sources

Inhibiting stearoyl‐CoA desaturase suppresses bone metastatic prostate cancer by modulating cellular stress, mTOR signaling, and DNA damage response

FEBS Letters, EarlyView.
Bone metastasis in prostate cancer (PCa) patients is a clinical hurdle due to the poor understanding of the supportive bone microenvironment. Here, we identify stearoyl‐CoA desaturase (SCD) as a tumor‐promoting enzyme and potential therapeutic target in bone metastatic PCa.
Alexis Wilson, Mackenzie K Herroon, Shane Mecca, Laimar C Garmo, Jacob Lindquist, Shrila Rajendran, Steve M. Patrick, Izabela Podgorski   +7 more
wiley   +1 more source

Substrate Specificity and Enzyme Recycling Using Chitosan Immobilized Laccase

Molecules, 2014
The immobilization of laccase (Aspergillus sp.) on chitosan by cross-linking and its application in bioconversion of phenolic compounds in batch reactors were studied.
Everton Skoronski, Mylena Fernandes, Maria de Lourdes Borba Magalhães, Gustavo Felippe da Silva, Jair Juarez João, Carlos Henrique Lemos Soares, Agenor Fúrigo Júnior   +6 more
doaj   +1 more source

Rational engineering of an elevator-type metal transporter ZIP8 reveals a conditional selectivity filter critically involved in determining substrate specificity

Communications Biology, 2023
Engineering of transporters to alter substrate specificity as desired holds great potential for applications, including metabolic engineering. However, the lack of knowledge on molecular mechanisms of substrate specificity hinders designing effective ...
Yuhan Jiang, Zhen Li, Dexin Sui, Gaurav Sharma, Tianqi Wang, Keith MacRenaris, Hideki Takahashi, Kenneth Merz, Jian Hu   +8 more
doaj   +1 more source

Substrate Specificity of Trypanothione Reductase [PDF]

European Journal of Biochemistry, 1997
Trypanothione redutase, one of the family of flavin‐dependent disulfide oxidoreductases, catalyses the reduction of trypanothione disulfide [N1,N8‐bis(glutathionyl)spermidine] and related glutathionyl‐polyamine disulfides. A series of subtly different, designed substrate analogues based on trypanothione were prepared by means of a solid‐phase approach ...
I R, Marsh, M, Bradley
openaire   +2 more sources

The (Glg)ABCs of cyanobacteria: modelling of glycogen synthesis and functional divergence of glycogen synthases in Synechocystis sp. PCC 6803

FEBS Letters, EarlyView.
We reconstituted Synechocystis glycogen synthesis in vitro from purified enzymes and showed that two GlgA isoenzymes produce glycogen with different architectures: GlgA1 yields denser, highly branched glycogen, whereas GlgA2 synthesizes longer, less‐branched chains.
Kenric Lee, Dimitrios Bekiari, Sofia Doello, Karl Forchhammer   +3 more
wiley   +1 more source

Structural biology of ferritin nanocages

FEBS Letters, EarlyView.
Ferritin is a conserved iron‐storage protein that sequesters iron as a ferric mineral core within a nanocage, protecting cells from oxidative damage and maintaining iron homeostasis. This review discusses ferritin biology, structure, and function, and highlights recent cryo‐EM studies revealing mechanisms of ferritinophagy, cellular iron uptake, and ...
Eloise Mastrangelo, Flavio Di Pisa
wiley   +1 more source

Organ‐specific redox imbalances in spinal muscular atrophy mice are partially rescued by SMN antisense oligonucleotides

FEBS Letters, EarlyView.
We identified a systemic, progressive loss of protein S‐glutathionylation—detected by nonreducing western blotting—alongside dysregulation of glutathione‐cycle enzymes in both neuronal and peripheral tissues of Taiwanese SMA mice. These alterations were partially rescued by SMN antisense oligonucleotide therapy, revealing persistent redox imbalance as ...
Sofia Vrettou, Brunhilde Wirth
wiley   +1 more source