Results 61 to 70 of about 467,506 (265)
Probing Steroidal Substrate Specificity of Cytochrome P450 BM3 Variants
Molecules, 2016 M01A82W, M11A82W and M01A82WS72I are three cytochrome P450 BM3 (CYP102A1) variants. They can catalyze the hydroxylation of testosterone (TES) and norethisterone at different positions, thereby making them promising biocatalysts for steroid hydroxylation.
Xing Liu +3 more
doaj +1 more source
The Substrate and Anomeric Specificity of Fructokinase
Journal of Biological Chemistry, 1973 Abstract Fructokinase from beef liver is shown to be specific for the β-furanose anomer of d-fructose (and the α anomer of l-sorbose) by showing that certain 2,5-anhydroalditols are substrates, while other 2,5-anhydro compounds, 2,6-anhydro-d-mannitol, and 2,6-anhydro-d-glucitol are not.
F M, Raushel, W W, Cleland
openaire +2 more sources
FEBS Letters, EarlyView.We present robust protocols for the preparation of supported lipid bilayers (SLBs) incorporating either Salmonella smooth LPS or outer membrane vesicles (OMVs). We use a combination of quartz crystal microbalance with dissipation (QCM‐D) and fluorescence microscopy to both characterize the SLBs of various compositions and to probe their interactions ...
Hudson P. Pace +6 more
wiley +1 more source
FEBS Letters, EarlyView.Proteostasis and the gut microbiota play a key role in shaping host physiology. Microbiota‐derived metabolites, vitamins, and RNA modulate host proteostasis. Findings from model systems, including C. elegans, indicate microbes can either stabilize or disrupt host proteostasis.
Abhishek Anil Dubey, Maria Ermolaeva
wiley +1 more source
Block-based characterization of protease specificity from substrate sequence profile
BMC Bioinformatics, 2017 Background The mechanism of action of proteases has been widely studied based on substrate specificity. Prior research has been focused on the amino acids at a single amino acid site, but rarely on combinations of amino acids around the cleavage bond ...
Enfeng Qi +4 more
doaj +1 more source
Structural basis for substrate specificity and catalysis of α1,6-fucosyltransferase
Nature Communications, 2020 Core-fucosylation of the N-glycan core is an essential biological modification and the α1,6- fucosyltransferase FUT8 is the only enzyme in humans that catalyses this modification through the addition of an α-1,6-linked fucose to N-glycans.
Ana García-García +6 more
doaj +1 more source
Phosphoinositides and inositol phosphates as molecular glues
FEBS Letters, EarlyView.Inositol phosphates (IPs) and phosphoinositides (PIPs) regulate diverse eukaryotic processes. Beyond recruiting signaling proteins or acting as structural cofactors, recent studies suggest they mediate protein–protein interactions as natural molecular glues.
Aleshia Seaton‐Terry +9 more
wiley +1 more source
Substrate Specificity and Enzyme Recycling Using Chitosan Immobilized Laccase
Molecules, 2014 The immobilization of laccase (Aspergillus sp.) on chitosan by cross-linking and its application in bioconversion of phenolic compounds in batch reactors were studied.
Everton Skoronski +6 more
doaj +1 more source
The substrate specificity of carnitine acetyltransferase [PDF]
Biochemical Journal, 1967 1. A study of the acyl group specificity of the carnitine acetyltransferase reaction [acyl-(-)carnitine+CoASH right harpoon over left harpoon (-)-carnitine+acyl-CoA] has been made with the enzyme from pigeon breast muscle. Acyl groups containing up to 10 carbon atoms are transferred and detailed kinetic investigations with a range of acyl-CoA and ...
openaire +2 more sources
Structural insights and therapeutic targets in Acinetobacter baumannii capsule biosynthesis
FEBS Letters, EarlyView.Hypervirulent KL49 A. baumannii's capsular polysaccharide contains the nonulosonic acid 8‐epi‐Leg5,7Ac2, synthesized by epimerization via ElaA, ElaB, and ElaC. Crystal structures of ElaA, ElaB, and ElaC reveal their role in CMP‐Leg5,7Ac2 synthesis and regioselective C8 epimerization.
Woo Cheol Lee +7 more
wiley +1 more source