Results 231 to 240 of about 54,156 (279)

Stoichiometric complexation of streptomyces subtilisin inhibitor and subtilisin

Journal of Protein Chemistry, 1991
Subtilisin (Sbt) and Streptomyces subtilisin inhibitor (SSI) were analyzed either alone or together using sodium dodecylsulfate (SDS)-polyacrylamide gel electrophoresis (PAGE). With all ratios of Sbt to SSI tested, the proteins formed a stoichiometric complex, and migrated abnormally at the top of the gel.
L O, Narhi, J, Connor, M F, Rohde, M G, McGinley, T, Arakawa   +4 more
openaire   +2 more sources

Thermal denaturation of Streptomyces subtilisin inhibitor, subtilisin BPN', and the inhibitor-subtilisin complex

Biochemistry, 1981
The thermal unfolding of the microbial proteinase inhibitor Streptomyces subtilisin inhibitor (SSI) [Sato, S., & Murao, S. (1973) Agric, Biol. Chem. 37, 1067-1074), the bacterial proteinase subtilisin BPN' (EC 3.4.21.14), and the complex formed by these two proteins has been studied by differential scanning calorimetry (DSC).
K, Takahashi, J M, Sturtevant
openaire   +2 more sources

Subtilisin

1997
Abstract The complete amino acid sequence of subtilisin E was deduced from the nucleotide sequences from chromosomal DNA isolated from B. subtilis 168 (Genbank accession number K01988; Ikemura et al., 1987) and is homologous with the sequences of subtilisin BPN’ (B. amyloliquefaciens; Stahl and Ferrari, 1984; accession number X00165) and
U Shinde, M Inouye
openaire   +1 more source

Alkaline-Resistance Model of Subtilisin ALP I, a Novel Alkaline Subtilisin

Journal of Biochemistry, 2001
The alkaline-resistance mechanism of the alkaline-stable enzymes is not yet known. To clarify the mechanism of alkaline-resistance of alkaline subtilisin, structural changes of two typical subtilisins, subtilisin ALP I (ALP I) and subtilisin Sendai (Sendai), were studied by means of physicochemical methods.
H, Maeda, O, Mizutani, Y, Yamagata, E, Ichishima, T, Nakajima   +4 more
openaire   +2 more sources

Reasoning Enantioselectivity and Kinetics of Seleno-Subtilisin from the Subtilisin Template

Archives of Biochemistry and Biophysics, 1998
The active-site serine (Ser221) of subtilisin Carlsberg(from Bacillus licheniformis) and subtilisin BPN' (fromBacillus amyloliquefaciens) was chemically converted into a selenocystein. Contrary to subtilisin's protease activity the semisynthetic seleno-subtilisin catalyzed the reduction of hydroperoxides.
D, Häring, B, Hubert, E, Schüler, P, Schreier   +3 more
openaire   +2 more sources

Subtilisin-Catalyzed Glycopeptide Condensation

2004
This method describes the use of subtilisin-catalyzed peptide condensation to form a 15-residue glycopeptide from two smaller synthetic peptides. A 12-residue peptide ester is synthesized by solid-phase peptide synthesis using a PAM-modified Rink amide resin that allows the formation of a peptide ester suitable for subtilisin ligation.
Thomas J, Tolbert, Chi-Huey, Wong
openaire   +2 more sources

Kinetics of subtilisin and thiolsubtilisin

Molecular and Cellular Biochemistry, 1983
Subtilisin is a bacterial serine protease with a broad specificity in the S1 subsite. It has been very extensively studied using a variety of kinetic and physical techniques. A chemical derivative, thiolsubtilisin, has been subjected to similar studies in order to analyze the effects of the OH to SH conversion on enzyme activity.
M, Philipp, M L, Bender
openaire   +2 more sources

Protein engineering of subtilisin

Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology, 2000
The serine protease subtilisin is an important industrial enzyme as well as a model for understanding the enormous rate enhancements affected by enzymes. For these reasons along with the timely cloning of the gene, ease of expression and purification and availability of atomic resolution structures, subtilisin became a model system for protein ...
openaire   +2 more sources