Results 51 to 60 of about 25,546 (199)

The Structure of Subtilisin Novo

Journal of Biological Chemistry, 1968
The amino acid composition of subtilisin Novo has been determined. The compositions of all of the 14 expected tryptic peptides from subtilisin Novo were obtained or deduced. A total of 281 residues was found by analysis of the protein, in good agreement with the total of 275 residues in the expected 14 tryptic peptides.
S A, Olaitan, R J, DeLange, E L, Smith
openaire   +2 more sources

Safety assessment of subtilisin QK in rats

BMC Pharmacology and Toxicology, 2021
Background Subtilisin QK is a serine protease in the subtilisin family, and is fermented by Bacillus subtilis QK02. The fibrinolytic activity of subtilisin QK was measured by detecting low molecular weight degradation products using a spectrophotometric ...
Shuai Xiao, Dingbang Hu, Ya Gao, Yang Ai, Sang Luo, Song Chen, Ben Wang, Li Zhou, Yanshan Dong, Yefu Wang   +9 more
doaj   +1 more source

The kunitz domain protein BLI-5 plays a functionally conserved role in cuticle formation in a diverse range of nematodes [PDF]

, 2010
The cuticle of parasitic nematodes performs many critical functions and is essential for proper development and for protection from the host immune response.
Antony P. Page, Bernard, Blaxter, Chu, Cook, Fessler, Ford, Frand, Ghedin, Gillian McCormack, Gillian Stepek, Hawdon, Johnstone, Johnstone, Kingston, Kingston, Kramerova, Kramerova, Kress, Mieszczanek, Milstone, Nagano, Novelli, Novelli, Paesen, Page, Page, Poole, Priess, Redmond, Rhoads, Sasaki, Scott, Shamansky, Skuce, Stanssens, Studier, Thacker, Thacker, Thacker, Thacker, Thein, Wells, Winter   +43 more
core   +1 more source

A novel familial mutation in the PCSK1 gene that alters the oxyanion hole residue of proprotein convertase 1/3 and impairs its enzymatic activity. [PDF]

, 2014
Four siblings presented with congenital diarrhea and various endocrinopathies. Exome sequencing and homozygosity mapping identified five regions, comprising 337 protein-coding genes that were shared by three affected siblings. Exome sequencing identified
Abbasi, Montaser, Bar-Oz, Benjamin, Berger, Itai, Blanco, Elias, Elpeleg, Orly, Lindberg, Iris, Martín, Martin G, Pappo, Orit, Shteyer, Eyal, Wilschanski, Michael, Yourshaw, Michael, Zangen, David   +11 more
core   +6 more sources

Pharmaceutically modified subtilisins withstand acidic conditions and effectively degrade gluten in vivo [PDF]

, 2019
Detoxification of gluten immunogenic epitopes is a promising strategy for the treatment of celiac disease. Our previous studies have shown that these epitopes can be degraded in vitro by subtilisin enzymes derived from Rothia mucilaginosa, a natural ...
Darwish, Ghassan, Helmerhorst, Eva J., Oppenheim, Frank G., Schuppan, Detlef, Wei, Guoxian   +4 more
core   +1 more source

"Fluctuograms" reveal the intermittent intra-protein communication in subtilisin Carlsberg and correlate mechanical coupling with co-evolution. [PDF]

PLoS Computational Biology, 2011
The mechanism of intra-protein communication and allosteric coupling is key to understanding the structure-property relationship of protein function. For subtilisin Carlsberg, the Ca²+-binding loop is distal to substrate-binding and active sites, yet the
Jordi Silvestre-Ryan, Yuchun Lin, Jhih-Wei Chu   +2 more
doaj   +1 more source

Toward an Alternative Intrinsic Probe for Spectroscopic Characterization of a Protein [PDF]

, 2010
The intrinsic fluorescent amino acid tryptophan is the unanimous choice for the spectroscopic investigation of proteins. However, several complicacies in the interpretation of tryptophan fluorescence in a protein are inevitable and an alternative ...
Goswami, Nirmal, Makhal, Abhinandan, Pal, Samir Kumar   +2 more
core   +1 more source

Genetic attenuation of ALDH1A1 increases metastatic potential and aggressiveness in colorectal cancer

Molecular Oncology, EarlyView.
Aldehyde dehydrogenase 1A1 (ALDH1A1) is a cancer stem cell marker in several malignancies. We established a novel epithelial cell line from rectal adenocarcinoma with unique overexpression of this enzyme. Genetic attenuation of ALDH1A1 led to increased invasive capacity and metastatic potential, the inhibition of proliferation activity, and ultimately ...
Martina Poturnajova, Zuzana Kozovska, Ondrej Pos, Kristina Pavlov, Sachin Gulati, Peter Makovicky, Kristina Jakic, Monika Burikova, Eva Sedlackova, Barbora Svitkova, Silvia Tyciakova, Vojtech Bystry, Nicolas Blavet, Boris Tichy, Matej Hrnciar, Jaroslav Budis, Miroslav Tomas, Peter Dubovan, Georgina Kolnikova, Veronika Repaska, Nikoleta Mojzesova, Eva Zomborska, Daniel Pindak, Michal Mego, Tomas Szemes, Miroslava Matuskova   +25 more
wiley   +1 more source

The hydrolysis of the three clupein components by subtilisin Novo, subtilisin Carlsberg and nitrated subtilisin Carlsberg [PDF]

Carlsberg Research Communications, 1976
The three components of clupein (YI, YII and Z) were hydrolyzed by subtilisin Carlsberg and subtilisin Novo. Subtilisin Novo hydrolyzed all three components much faster than did subtilisin Carlsberg, but components YII and Z were hydrolyzed with the same initial rate and faster than component YI.
openaire   +1 more source

Directed evolution of enzymes at the crossroads of tradition and innovation

FEBS Open Bio, EarlyView.
An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova, Andrej Mirossay, Erik Sedlak   +2 more
wiley   +1 more source