Results 101 to 110 of about 16,065 (253)

Abundant Lysine Methylation and N-Terminal Acetylation in Sulfolobus islandicus Revealed by Bottom-Up and Top-Down Proteomics*

Molecular & Cellular Proteomics, 2016
Protein post-translational methylation has been reported to occur in archaea, including members of the genus Sulfolobus, but has never been characterized on a proteome-wide scale.
E. Vorontsov, E. Rensen, D. Prangishvili, M. Krupovic, J. Chamot-Rooke   +4 more
semanticscholar   +1 more source

An Unexpected Facet of Extremophiles: Their Aesthetic Potential in Artistic Expression

Microbial Biotechnology, Volume 18, Issue 10, October 2025.
In an era marked by environmental challenges and the proliferation of misinformation, the fusion of art and science is a strategy to promote public understanding and appreciation of complex scientific phenomena. We illustrate how the use of extremophiles offers novel avenues for artistic exploration and emphasise the benefits of such interdisciplinary ...
Luis Andrés Yarzábal Rodríguez, Lenys M. Buela Salazar, Yordanis Pérez‐Llano, Nina Gunde‐Cimerman, Ramón Alberto Batista‐García   +4 more
wiley   +1 more source

Quantifying Soil Microbiome Abundance by Metatranscriptomics and Complementary Molecular Techniques—Cross‐Validation and Perspectives

Molecular Ecology Resources, Volume 25, Issue 7, October 2025.
ABSTRACT Linking meta‐omics and biogeochemistry approaches in soils has remained challenging. This study evaluates the use of an internal RNA extraction standard and its potential for making quantitative estimates of a given microbial community size (biomass) in soil metatranscriptomics. We evaluate commonly used laboratory protocols for RNA processing,
Mathilde Borg Dahl, Stella Brachmann, Andrea Söllinger, Marina Schnell, Laureen Ahlers, Magdalena Wutkowska, Katharina J. Hoff, Neetika Nath, Verena Groß, Haitao Wang, Micha Weil, Marc Piecha, Marc Schaffer, Corinna Jensen, Andreas W. Kuss, Christoph Gall, Erika Wimmer, Thomas Pribasnig, Alexander Tøsdal Tveit, Bjarni D. Sigurdsson, Christa Schleper, Andreas Richter, Tim Urich   +22 more
wiley   +1 more source

Transcriptome changes in STSV2‐infected Sulfolobus islandicus REY15A undergoing continuous CRISPR spacer acquisition

Molecular Microbiology, 2016
A transcriptome study was performed on Sulfolobus islandicus REY15A actively undergoing CRISPR spacer acquisition from the crenarchaeal monocaudavirus STSV2 in rich and basal media over a 6 day period.
Carlos León-Sobrino, W. Kot, R. Garrett
semanticscholar   +1 more source

Improvement of a Sulfolobus-E. coli Shuttle Vector for Heterologous Gene Expression in Sulfolobus acidocaldarius

Journal of Microbiology and Biotechnology, 2015
A Sulfolobus-E. coli shuttle vector for an efficient expression of the target gene in S. acidocaldarius strain was constructed. The plasmid-based vector pSM21 and its derivative pSM21N were generated based on the pUC18 and Sulfolobus cryptic plasmid pRN1. They carried the S.
Sungmin, Hwang, Kyoung-Hwa, Choi, Naeun, Yoon, Jaeho, Cha   +3 more
openaire   +2 more sources

Quorum Sensing Inhibits Type III‐A CRISPR‐Cas System Activity Through Repressing Positive Regulators SarA and ArcR in Staphylococcus Aureus

Advanced Science, Volume 12, Issue 36, September 25, 2025.
This study proposes a novel model illustrating how quorum sensing (QS) regulates the type III‐A CRISPR‐Cas system in Staphylococcus aureus. The QS regulator AgrA suppresses CRISPR‐Cas activity by downregulating the transcriptional regulators SarA and ArcR.
Yang Li, Yuanyue Tang, Xiaofei Li, Nina Molin Høyland‐Kroghsbo, Hanne Ingmer, Xinan Jiao, Qiuchun Li   +6 more
wiley   +1 more source

DNA-Interacting Characteristics of the Archaeal Rudiviral Protein SIRV2_Gp1

Viruses, 2017
Whereas the infection cycles of many bacterial and eukaryotic viruses have been characterized in detail, those of archaeal viruses remain largely unexplored.
Eveline Peeters, Maarten Boon, Clare Rollie, Ronnie G. Willaert, Marleen Voet, Malcolm F. White, David Prangishvili, Rob Lavigne, Tessa E.F. Quax   +8 more
doaj   +1 more source

First crenarchaeal chitinase found in Sulfolobus tokodaii

Microbiological Research, 2012
This is the first description of a functional chitinase gene within the crenarchaeotes. Here we report of the heterologues expression of the ORF BAB65950 from Sulfolobus tokodaii in E. coli. The resulting protein degraded chitin and was hence classified as chitinase (EC 3.2.4.14).
Staufenberger, Tim, Imhoff, Johannes F., Labes, Antje   +2 more
openaire   +3 more sources

Diverse CRISPR-Cas responses and dramatic cellular DNA changes and cell death in pKEF9-conjugated Sulfolobus species

Nucleic Acids Research, 2016
The Sulfolobales host a unique family of crenarchaeal conjugative plasmids some of which undergo complex rearrangements intracellularly. Here we examined the conjugation cycle of pKEF9 in the recipient strain Sulfolobus islandicus REY15A.
Guannan Liu, Q. She, R. Garrett
semanticscholar   +1 more source

Virus-Induced Dormancy in the Archaeon Sulfolobus islandicus

mBio, 2015
We investigated the interaction between Sulfolobus spindle-shaped virus (SSV9) and its native archaeal host Sulfolobus islandicus. We show that upon exposure to SSV9, S.
M. Bautista, Changyi Zhang, Rachel J. Whitaker   +2 more
semanticscholar   +1 more source