Results 81 to 90 of about 13,977 (211)

Tn‐seq of Thermus thermophilus Genome Reveals Unexpected Tolerance to Insertions in Bacterial Common Essential Genes

MicrobiologyOpen, Volume 15, Issue 2, April 2026.
Tn‐seq analysis of Thermus thermophilus genome reveals insertions in almost all genes, this is, there is no clear discrimination of essential genes. A similar result has not been reported in other Tn‐seq analysis of bacterial genomes so far. The polyploidy of the Thermus genome could explain the apparent non‐essentiality of key genes.
Cristina L. Gómez‐Campo, Marc Gost, Bruna Fernanda Silva de Sousa, Laura Álvarez, José Berenguer, Modesto Redrejo‐Rodríguez, Mario Mencía   +6 more
wiley   +1 more source

Harnessing hyperthermostable lactonase from Sulfolobus solfataricus for biotechnological applications

Scientific Reports, 2016
Extremozymes have gained considerable interest as they could meet industrial requirements. Among these, SsoPox is a hyperthermostable enzyme isolated from the archaeon Sulfolobus solfataricus.
B. Rémy, Laure Plener, L. Poirier, M. Elias, D. Daudé, E. Chabrière   +5 more
semanticscholar   +1 more source

Molecular analysis of 3D domain swapping in the acylphosphatase from Escherichia coli

Acta Crystallographica Section D, Volume 82, Issue 4, Page 336-347, April 2026.
Structures of the monomer and intertwined dimer of the acylphosphatase from E. coli shed light on the molecular basis of its 3D domain swapping.Three‐dimensional domain swapping is a mechanism by which proteins form oligomers. At present, the molecular basis that dictates whether some proteins fold in their monomeric form or as intertwined oligomers is
Sergio Martínez-Rodríguez, Jose A. Gavira, M. Carmen Salinas-Garcia, Montserrat Andujar-Sánchez, Ana Camara-Artigas   +4 more
wiley   +1 more source

Bioleaching of Molybdenum by Two New Thermophilic Strains Isolated and Characterized [PDF]

Iranian Journal of Chemistry & Chemical Engineering, 2017
This study involves the isolation and characterization of a bacterial strain capable of bioleaching molybdenum ore. Bacterial growth was observed when rock sample was incubated in 9K at 70 ºC.
Mahshid Roshani, Seyed Abbas Shojaosadati, Seyed Jaber Safdari, Ebrahim Vasheghani-Farahani, Kazem Mirjalili, Zahra Manafi   +5 more
doaj  

The return of metabolism: biochemistry and physiology of glycolysis

Biological Reviews, Volume 101, Issue 2, Page 751-803, April 2026.
ABSTRACT Glycolysis is a fundamental metabolic pathway central to the bioenergetics and physiology of virtually all living organisms. In this comprehensive review, we explore the intricate biochemical principles and evolutionary origins of glycolytic pathways, from the classical Embden–Meyerhof–Parnas (EMP) pathway in humans to various prokaryotic and ...
Nana‐Maria Grüning, Federica Agostini, Camila Caldana, Johannes Hartl, Matthias Heinemann, Markus A. Keller, Jan Lukas Krüsemann, Costanza Lamperti, Carole L. Linster, Steffen N. Lindner, Julia Muenzner, Jens Nielsen, Zoran Nikoloski, Bettina Siebers, Jacky L. Snoep, Hezi Tenenboim, Bas Teusink, Spencer J. Williams, Mirjam M. C. Wamelink, Markus Ralser   +19 more
wiley   +1 more source

Exploring surface structures

eLife
The surface layer of Sulfolobus acidocaldarius consists of a flexible but stable outer protein layer that interacts with an inner, membrane-bound protein.
Bernhard Schuster
doaj   +1 more source

CRISPR-mediated gene silencing reveals involvement of the archaeal S-layer in cell division and virus infection

Nature Communications, 2019
The S-layer is a proteinaceous envelope often found in bacterial and archaeal cells. Here, the authors use CRISPR-based technology to silence slaB, encoding the S-layer membrane anchor, to show that an intact S-layer is important for cell division and ...
Isabelle Anna Zink, Kevin Pfeifer, Erika Wimmer, Uwe B. Sleytr, Bernhard Schuster, Christa Schleper   +5 more
doaj   +1 more source

A genetic study of SSV1, the prototypical fusellovirus.

Frontiers in Microbiology, 2012
Viruses of thermophilic Archaea are unique in both their structures and genomic sequences. The most widespread and arguably best studied are the lemon-shaped fuselloviruses.
Eric eIverson, Kenneth eStedman
doaj   +1 more source

Abundant Lysine Methylation and N-Terminal Acetylation in Sulfolobus islandicus Revealed by Bottom-Up and Top-Down Proteomics*

Molecular & Cellular Proteomics, 2016
Protein post-translational methylation has been reported to occur in archaea, including members of the genus Sulfolobus, but has never been characterized on a proteome-wide scale.
E. Vorontsov, E. Rensen, D. Prangishvili, M. Krupovic, J. Chamot-Rooke   +4 more
semanticscholar   +1 more source

Protein Language Model‐Guided Engineering of a 2,3‐Butanediol Dehydrogenase for the Enantioselective Synthesis of Cyclic α‐Hydroxy Ketones

Advanced Science, Volume 13, Issue 18, 27 March 2026.
A (2R,3R)‐butanediol dehydrogenase from Bacillus subtilis (BsBDH) is engineered for the enantioselective synthesis of 2‐hydroxycyclohexanone. A PASS computational design strategy is proposed to enhance the thermostability of BsBDH. Moreover, ESM‐1v combined with ISM is utilized for enhancing and inverting its stereoselectivity.
Haote Ding, Ling Jiang, Yijia Song, Zhongji Pu, Lirong Yang, Haoran Yu   +5 more
wiley   +1 more source