Results 61 to 70 of about 24,088 (253)
Proteins: Structure, Function, and Bioinformatics, EarlyView.ABSTRACT We present the first x‐ray crystallographic structural evidence of an archaeal DNA ligase showing the AMP covalent adduct together with further cofactor hydrolysis, capturing a transient intermediary in the first step of the ligation reaction, triggered by the pyrophosphate hydrolysis.
A. X. Quintana‐Armas +3 more
wiley +1 more source
The Sulfolobus solfataricus P2 genome project
FEBS Letters, 1996 Over 800 kbp of the 3‐Mbp genome of Sulfolobus solfataricus have been sequenced to date. Our approach is to sequence subclones of mapped cosmids, followed by sequencing directly on cosmid templates with custom primers. Using a prototype automated system for genome‐scale analysis, known as MAGPIE, along with other tools, we have discovered one open ...
Charlebois, RL +4 more
openaire +3 more sources
An efficient one-step site-directed deletion, insertion, single and multiple-site plasmid mutagenesis protocol [PDF]
, 2012 Background: Mutagenesis plays an essential role in molecular biology and biochemistry. It has also been used in enzymology and protein science to generate proteins which are more tractable for biophysical techniques.
Liu, Huanting, Naismith, James Henderson
core +1 more source
Proteins: Structure, Function, and Bioinformatics, EarlyView.ABSTRACT BorF is a short‐chain flavin reductase from a desert soil bacterium that uses NADH to reduce FAD to FADH2, which is used by the tryptophan‐6‐halogenase BorH to chlorinate tryptophan in the biosynthetic pathway of borregomycin A. The X‐ray crystal structure of BorF bound to FAD was solved to 2.37 Å by molecular replacement.
Zheng Ma +3 more
wiley +1 more source
Acta Biochimica et Biophysica Sinica, 2022 Apurinic/apyrimidic (AP) sites are severe DNA damages and strongly block DNA extension by major DNA polymerases. Y-family DNA polymerases possess a strong ability to bypass AP sites and continue the DNA synthesis reaction, which is called translesion ...
Wang Weiwei +7 more
doaj +1 more source
S‐Adenosylmethionine (SAM) hydrolases counter increased SAM epimerisation in thermophilic archaea
The FEBS Journal, EarlyView.S‐Adenosyl‐l‐methionine (SAM) is a vital enzyme cofactor. Epimerisation at the sulfonium centre of biologically active (SS,SCα)‐SAM is driven by heat, yielding biologically inactive (RS,SCα)‐SAM. Here, two novel archaeal SAM hydrolases from the thermophilic Sulfolobus acidocaldarius and the halophilic Haloferax volcanii are shown to cleave (RS,SCα)‐SAM.
Agnes Bartels +7 more
wiley +1 more source
Bioenergetics of the archaebacterium Sulfolobus
Biochimica et Biophysica Acta (BBA) - Bioenergetics, 1996 Archaea are forming one of the three kingdoms defining the universal phylogenetic tree of living organisms. Within itself this kingdom is heterogenous regarding the mechanisms for deriving energy from the environment for support of cellular functions.
openaire +2 more sources
Structure of Csx1-cOA4 complex reveals the basis of RNA decay in Type III-B CRISPR-Cas
Nature Communications, 2019 Type III CRISPR-Cas RNases from the Csm and Csx families are activated by cyclic oligoadenylates (cOA). Here the authors present the cOA bound Sulfolobus islandicus Csx1 structure, which forms a hexamer and reveal an allosteric mechanism for the ...
Rafael Molina +8 more
doaj +1 more source
Phylogenetic differences in content and intensity of periodic proteins [PDF]
, 2005 Many proteins exhibit sequence periodicity, often correlated with a visible structural periodicity. The statistical significance of such periodicity can be assessed by means of a chi-square-based test, with significance thresholds being calculated from ...
Gatherer, D., McEwan, N.R.
core +1 more source
MicrobiologyOpen, Volume 15, Issue 2, April 2026.Tn‐seq analysis of Thermus thermophilus genome reveals insertions in almost all genes, this is, there is no clear discrimination of essential genes. A similar result has not been reported in other Tn‐seq analysis of bacterial genomes so far. The polyploidy of the Thermus genome could explain the apparent non‐essentiality of key genes.
Cristina L. Gómez‐Campo +6 more
wiley +1 more source