Exopolysaccharide composition and size in Sulfolobus acidocaldarius biofilms [PDF]
Frontiers in Microbiology, 2022 Extracellular polymeric substances (EPS) comprise mainly carbohydrates, proteins and extracellular DNA (eDNA) in biofilms formed by the thermoacidophilic Crenarchaeon Sulfolobus acidocaldarius. However, detailed information on the carbohydrates in the S.
Laura Kuschmierz +7 more
doaj +7 more sources
5′-untranslated region sequences enhance plasmid-based protein production in Sulfolobus acidocaldarius [PDF]
Frontiers in MicrobiologySulfolobus acidocaldarius, a thermoacidophilic archaeon of the phylum Thermoproteota (former Crenarchaeota), is a widely used model organism for gene deletion studies and recombinant protein production.
Laura Kuschmierz +6 more
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Impact of Nutrient Excess on Physiology and Metabolism of Sulfolobus acidocaldarius [PDF]
Frontiers in MicrobiologyOverflow metabolism is a well-known phenomenon that describes the seemingly wasteful and incomplete substrate oxidation by aerobic cells, such as yeasts, bacteria, and mammalian cells, even when conditions allow for total combustion via respiration. This
Yannick Gansemans +2 more
exaly +5 more sources
Role of VapBC4 Toxin-Antitoxin System of Sulfolobus acidocaldarius in Heat Stress Adaptation. [PDF]
MBioToxin-antitoxin (TA) systems are important for stress adaptation in prokaryotes, including persistence, antibiotic resistance, pathogenicity, and biofilm formation.
Abhrajyoti Ghosh +2 more
exaly +5 more sources
Effects of genomic location on ectopic integration and gene expression of a reporter gene cassette in Sulfolobus acidocaldarius [PDF]
Frontiers in MicrobiologyIn eukaryotes and bacteria, it is well-established that the genomic location of ectopic gene integration influences the expression level due to replication-associated gene dosage effects as well as effects mediated by chromatin organization. In contrast,
Yifei Xu +5 more
doaj +3 more sources
Application of the endogenous CRISPR-Cas type I-D system for genetic engineering in the thermoacidophilic archaeon Sulfolobus acidocaldarius [PDF]
Frontiers in Microbiology, 2023 CRISPR (clustered regularly interspaced short palindromic repeats)-Cas systems are widely distributed among bacteria and archaea. In this study, we demonstrate the successful utilization of the type I-D CRISPR-Cas system for genetic engineering in the ...
Jan Bost +5 more
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High-Temperature Live-Cell Imaging of Cytokinesis, Cell Motility, and Cell-Cell Interactions in the Thermoacidophilic Crenarchaeon Sulfolobus acidocaldarius [PDF]
Frontiers in Microbiology, 2021 Significant technical challenges have limited the study of extremophile cell biology. Here we describe a system for imaging samples at 75°C using high numerical aperture, oil-immersion lenses.
Arthur Charles-Orszag +2 more
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Lipid hydrogen isotope compositions primarily reflect growth water in the model archaeon Sulfolobus acidocaldarius [PDF]
Applied and Environmental MicrobiologyThe stable hydrogen isotope composition (δ2H) of lipid biomarkers can track environmental processes and remain stable over geologically relevant time scales, enabling studies of past climate, hydrology, and ecology.
Carolynn M. Harris +12 more
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An unusual glycerol-3-phosphate dehydrogenase in Sulfolobus acidocaldarius elucidates the diversity of glycerol metabolism across Archaea [PDF]
Communications BiologyGlycerol is highly abundant in natural ecosystems and serves as both an important carbon source for microorganisms as well as a promising feedstock for industrial applications.
Christian Schmerling +14 more
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Sulfolobus acidocaldarius, half a century of archaeal research [PDF]
Journal of BacteriologySince its isolation from a Yellowstone hot spring in 1972, Sulfolobus acidocaldarius has become one of the most important model organisms for archaeal biology. Initially studied for its remarkable adaptation to high temperature and low pH, it has evolved
Marleen van Wolferen +3 more
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