Results 1 to 10 of about 14,486 (168)

Coordination of chromosome segregation and cell division in the archaeon Sulfolobus acidocaldarius [PDF]

Nature Communications
Despite its fundamental importance for the propagation of all cellular life, very little is known about the molecular basis of chromosome segregation in archaea.
Rachel Y. Samson, Naomichi Takemata, Stephen D. Bell   +2 more
doaj   +2 more sources

The Effects of Temperature and Growth Phase on the Lipidomes of Sulfolobus islandicus and Sulfolobus tokodaii

Life, 2015
The functionality of the plasma membrane is essential for all organisms. Adaption to high growth temperatures imposes challenges and Bacteria, Eukarya, and Archaea have developed several mechanisms to cope with these.
Alexander H. Treusch, Martin Brandl, Christer S Ejsing   +2 more
exaly   +3 more sources

Evolutionary conservation of ubiquitin-like protein urmylation as revealed by URM1 gene shuffle from archaea to yeast [PDF]

Communications Biology
Urm1 from yeast is a unique ubiquitin-like protein with dual functionality. It has been shown to operate in tRNA thiolation and protein urmylation, combining features typical of bacterial sulfur carriers and classical ubiquitin-like modifiers.
Katharina Zupfer, Lars Kaduhr, Larissa Bessler, Mark Helm, Raffael Schaffrath   +4 more
doaj   +2 more sources

Archaeal GPN-loop GTPases involve a lock-switch-rock mechanism for GTP hydrolysis

mBio, 2023
Three GPN-loop GTPases, GPN1–GPN3, are central to the maturation and trafficking of eukaryotic RNA polymerase II. This GTPase family is widely represented in archaea but typically occurs as single paralogs.
Lukas Korf, Xing Ye, Marian S. Vogt, Wieland Steinchen, Mohamed Watad, Chris van der Does, Maxime Tourte, Shamphavi Sivabalasarma, Sonja-Verena Albers, Lars-Oliver Essen   +9 more
doaj   +1 more source

First Isolation and Structure Elucidation of GDNT‐β‐Glu – Tetraether Lipid Fragment from Archaeal Sulfolobus Strains

ChemistryOpen, 2021
Due to their special chemical structure, tetraether lipids (TEL) represent essential elements of archaeal membranes, providing these organisms with extraordinary properties.
Dr. Alexander Scholte, Christoph Hübner, Dr. Dieter Ströhl, Olaf Scheufler, Dr. Steffen Czich, Julia M. Börke, Dr. Gerhard Hildebrand, Prof. Dr. Klaus Liefeith   +7 more
doaj   +1 more source

Chromosome conformation capture assay combined with biotin enrichment for hyperthermophilic archaea

STAR Protocols, 2021
Summary: Chromosome organization in archaea has long been enigmatic due, in part, to the typically small cell size of archaea and the extremophilic nature of many of the model archaeal species studies, rendering live-cell imaging technically challenging.
Naomichi Takemata, Stephen D. Bell
doaj   +1 more source

Expression, purification, and characterization of a membrane-associated cyclic oligo-adenylate degrader from Sulfolobus islandicus

STAR Protocols, 2021
Summary: Type III CRISPR-cas systems initiate cyclic oligo-adenylate (cOA) signaling to initiate immune response. Previously, we identified that a membrane-associated DHH-DHHA1 family protein from Sulfolobus islandicus efficiently degrades cOA.
Ruiliang Zhao, Yang Yang, Ke Yang, Wenyuan Han   +3 more
doaj   +1 more source

High-resolution analysis of chromosome conformation in hyperthermophilic archaea

STAR Protocols, 2021
Summary: Chromosome conformation capture (3C) techniques are emerging as promising approaches to study genome organization in Archaea, the least understood domain of life in terms of chromosome biology. Here, we describe a 3C technique combined with deep
Naomichi Takemata, Stephen D. Bell
doaj   +1 more source

Species-Specific Recognition of Sulfolobales Mediated by UV-Inducible Pili and S-Layer Glycosylation Patterns

mBio, 2020
The UV-inducible pili system of Sulfolobales (Ups) mediates the formation of species-specific cellular aggregates. Within these aggregates, cells exchange DNA to repair DNA double-strand breaks via homologous recombination. Substitution of the Sulfolobus
Marleen van Wolferen, Asif Shajahan, Kristina Heinrich, Susanne Brenzinger, Ian M. Black, Alexander Wagner, Ariane Briegel, Parastoo Azadi, Sonja-Verena Albers   +8 more
doaj   +1 more source

Biochemical characterization of a structure-specific resolving enzyme from Sulfolobus islandicus rod-shaped virus 2. [PDF]

PLoS ONE, 2011
Sulfolobus islandicus rod shaped virus 2 (SIRV2) infects the archaeon Sulfolobus islandicus at extreme temperature (70°C-80°C) and acidity (pH 3). SIRV2 encodes a Holliday junction resolving enzyme (SIRV2 Hjr) that has been proposed as a key enzyme in ...
Andrew F Gardner, Chudi Guan, William E Jack   +2 more
doaj   +1 more source