Optimization of an in vitro transcription/translation system based on Sulfolobus solfataricus cell lysate. [PDF]
A system is described which permits the efficient synthesis of proteins in vitro at high temperature. It is based on the use of an unfractionated cell lysate (S30) from Sulfolobus solfataricus previously well characterized in our laboratory for ...
Anna La Teana, +5 more
core +2 more sources
Replication-biased genome organisation in the crenarchaeon
Background Species of the crenarchaeon Sulfolobus harbour three replication origins in their single circular chromosome that are synchronously initiated during replication.
Lundgren Magnus +5 more
doaj +1 more source
Electron cryo-microscopy reveals the structure of the archaeal thread filament
Pili are filamentous appendages on the surface of bacteria and archaea, and play roles in multiple processes such as adhesion, motility and horizontal gene transfer. Here, Gaines et al. describe the structure of a new type of pilus, termed ‘thread’, from
Matthew C. Gaines +10 more
doaj +1 more source
Aerobic Lineage of the Oxidative Stress Response Protein Rubrerythrin Emerged in an Ancient Microaerobic, (Hyper)Thermophilic Environment [PDF]
Indexación: Web of Science; Scopus.Rubrerythrins (RBRs) are non-heme di-iron proteins belonging to the ferritin-like superfamily. They are involved in oxidative stress defense as peroxide scavengers in a wide range of organisms. The vast majority of RBRs,
Cardenas, JP, Holmes, DS, Quatrini, R
core +1 more source
Endogenous Mutagenesis in Recombinant Sulfolobus Plasmids [PDF]
ABSTRACT Low rates of replication errors in chromosomal genes of Sulfolobus spp. demonstrate that these extreme thermoacidophiles can maintain genome integrity in environments with high temperature and low pH. In contrast to this genetic stability, we observed unusually frequent mutation of the β-
Cynthia J, Sakofsky, Dennis W, Grogan
openaire +2 more sources
Only a few archaeal filamentous viruses have been structurally characterized. Here the authors describe the membrane-enveloped Sulfolobus filamentous virus 1 that infects Sulfolobus shibatae and present its 3.7 Å resolution cryo-EM structure, which ...
Ying Liu +7 more
doaj +1 more source
Exploring the mechanism of formation of native-like and precursor amyloid oligomers for the native acylphosphatase from Sulfolobus solfataricus [PDF]
Over 40 human diseases are associated with the formation of well-defined proteinaceous fibrillar aggregates. Since the oligomers precursors to the fibrils are increasingly recognized to be the causative agents of such diseases, it is important to ...
BEMPORAD F +5 more
core +1 more source
A comprehensive analysis of the importance of translation initiation factors for Haloferax volcanii applying deletion and conditional depletion mutants [PDF]
Translation is an important step in gene expression. The initiation of translation is phylogenetically diverse, since currently five different initiation mechanisms are known.
Gäbel, Katrin +4 more
core +7 more sources
Thermal stability and aggregation of sulfolobus solfataricus b-glycosidase are dependent upon the N-e-methylation of specific lysyl residues: critical role of in vivo post-translational modifications. [PDF]
Methylation in vivo is a post-translational modification observed in several organisms belonging to eucarya, bacteria, and archaea. Although important implications of this modification have been demonstrated in several eucaryotes, its biological role in ...
A. ANDOLFO +10 more
core +1 more source
UDP-sulfoquinovose formation by Sulfolobus acidocaldarius [PDF]
Extremophiles, 19 (2)
Zolghadr, Behnam +14 more
openaire +3 more sources

