Structural–Functional Relationship of the Ribonucleolytic Activity of aIF5A from Sulfolobus solfataricus [PDF]
Biomolecules, 2022 The translation factor IF5A is a highly conserved protein playing a well-recognized and well-characterized role in protein synthesis; nevertheless, some of its features as well as its abundance in the cell suggest that it may perform additional functions
Alice Romagnoli +8 more
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Insights Into the Binding Mechanism of GC7 to Deoxyhypusine Synthase in Sulfolobus solfataricus: A Thermophilic Model for the Design of New Hypusination Inhibitors [PDF]
Frontiers in Chemistry, 2020 Translation factor 5A (eIF5A) is one of the most conserved proteins involved in protein synthesis. It plays a key role during the elongation of polypeptide chains, and its activity is critically dependent on hypusination, a post-translational ...
Mattia D'Agostino +9 more
doaj +2 more sources
Optimization of an In Vitro Transcription/Translation System Based on Sulfolobus solfataricus Cell Lysate. [PDF]
Archaea, 2019 A system is described which permits the efficient synthesis of proteins in vitro at high temperature. It is based on the use of an unfractionated cell lysate (S30) from Sulfolobus solfataricus previously well characterized in our laboratory for ...
Lo Gullo G +5 more
europepmc +4 more sources
Interplay between Alba and Cren7 Regulates Chromatin Compaction in Sulfolobus solfataricus [PDF]
Biomolecules, 2022 Chromatin compaction and regulation are essential processes for the normal function of all organisms, yet knowledge on how archaeal chromosomes are packed into higher-order structures inside the cell remains elusive.
Marc Kenneth M. Cajili, Eloise I. Prieto
doaj +2 more sources
Crystal structure of the translation recovery factor Trf from Sulfolobus solfataricus [PDF]
FEBS Open Bio, 2020 During translation initiation, the heterotrimeric archaeal translation initiation factor 2 (aIF2) recruits the initiator tRNAi to the small ribosomal subunit.
Marco Kaiser +5 more
doaj +2 more sources
The transcript cleavage factor paralogue TFS4 is a potent RNA polymerase inhibitor [PDF]
Nature Communications, 2017 Transcript cleavage factors such as eukaryotic TFIIS assist the resumption of transcription following RNA pol II backtracking. Here the authors find that one of the Sulfolobus solfataricus TFIIS homolog—TFS4—has evolved into a potent RNA polymerase ...
Thomas Fouqueau +5 more
doaj +3 more sources
The Carbon Switch at the Level of Pyruvate and Phosphoenolpyruvate in Sulfolobus solfataricus P2 [PDF]
Frontiers in Microbiology, 2019 Sulfolobus solfataricus P2 grows on different carbohydrates as well as alcohols, peptides and amino acids. Carbohydrates such as D-glucose or D-galactose are degraded via the modified, branched Entner–Doudoroff (ED) pathway whereas growth on peptides ...
Patrick Haferkamp +6 more
doaj +2 more sources
Intrinsic sequence specificity of the Cas1 integrase directs new spacer acquisition [PDF]
eLife, 2015 The adaptive prokaryotic immune system CRISPR-Cas provides RNA-mediated protection from invading genetic elements. The fundamental basis of the system is the ability to capture small pieces of foreign DNA for incorporation into the genome at the CRISPR ...
Clare Rollie +4 more
doaj +6 more sources
Systems biology of the modified branched Entner-Doudoroff pathway in Sulfolobus solfataricus. [PDF]
PLoS ONE, 2017 Sulfolobus solfataricus is a thermoacidophilic Archaeon that thrives in terrestrial hot springs (solfatares) with optimal growth at 80°C and pH 2-4. It catabolizes specific carbon sources, such as D-glucose, to pyruvate via the modified Entner-Doudoroff (
Ana Sofia Figueiredo +8 more
doaj +2 more sources
Modification of translation factor aIF5A from Sulfolobus solfataricus. [PDF]
Extremophiles, 2018 Eukaryotic eIF5A and its bacterial orthologue EF-P are translation elongation factors whose task is to rescue ribosomes from stalling during the synthesis of proteins bearing particular sequences such as polyproline stretches. Both proteins are characterized by unique post-translational modifications, hypusination and lysinylation, respectively, which ...
Bassani F +8 more
europepmc +5 more sources