Results 31 to 40 of about 5,022 (174)

Peptidyl-tRNA hydrolase from Sulfolobus solfataricus [PDF]

open access: yesNucleic Acids Research, 2003
An enzyme capable of liberating functional tRNA(Lys) from Escherichia coli diacetyl-lysyl-tRNA(Lys) was purified from the archae Sulfolobus solfataricus. Contrasting with the specificity of peptidyl- tRNA hydrolase (PTH) from E.coli, the S.solfataricus enzyme readily accepts E.coli formyl-methionyl-tRNA(fMet) as a substrate.
Fromant, Michel   +3 more
openaire   +3 more sources

The transcript cleavage factor paralogue TFS4 is a potent RNA polymerase inhibitor

open access: yesNature Communications, 2017
Transcript cleavage factors such as eukaryotic TFIIS assist the resumption of transcription following RNA pol II backtracking. Here the authors find that one of the Sulfolobus solfataricus TFIIS homolog—TFS4—has evolved into a potent RNA polymerase ...
Thomas Fouqueau   +5 more
doaj   +1 more source

A genetic study of SSV1, the prototypical fusellovirus.

open access: yesFrontiers in Microbiology, 2012
Viruses of thermophilic Archaea are unique in both their structures and genomic sequences. The most widespread and arguably best studied are the lemon-shaped fuselloviruses.
Eric eIverson, Kenneth eStedman
doaj   +1 more source

A Redox-Neutral, Two-Enzyme Cascade for the Production of Malate and Gluconate from Pyruvate and Glucose

open access: yesApplied Sciences, 2021
A triple mutant of NADP(H)-dependent malate dehydrogenase from thermotolerant Thermococcus kodakarensis has an altered cofactor preference for NAD+, as well as improved malate production compared to wildtype malate dehydrogenase.
Ravneet Mandair   +2 more
doaj   +1 more source

Merging Biocatalysis and Chemocatalysis in Flow: State‐of‐the‐Art and Future Directions for Sustainable Synthesis

open access: yesAngewandte Chemie, Volume 138, Issue 25, 15 June 2026.
This review highlights recent advances in integrating biocatalysis and chemocatalysis in continuous flow to create streamlined, sustainable processes. It examines chemo‐enzymatic cascades combining at least one enzymatic and one chemical step, discusses challenges such as enzyme immobilization, leaching, and reactor clogging, and presents solutions ...
Petros Siasiaridis   +2 more
wiley   +2 more sources

Intrinsic sequence specificity of the Cas1 integrase directs new spacer acquisition

open access: yeseLife, 2015
The adaptive prokaryotic immune system CRISPR-Cas provides RNA-mediated protection from invading genetic elements. The fundamental basis of the system is the ability to capture small pieces of foreign DNA for incorporation into the genome at the CRISPR ...
Clare Rollie   +4 more
doaj   +1 more source

The Chromosome Replication Machinery of the Archaeon Sulfolobus solfataricus [PDF]

open access: yesJournal of Biological Chemistry, 2006
In the three domains of life, the archaea, bacteria, and eukarya, there are two general lineages of DNA replication proteins: the bacterial and the eukaryal/archaeal lineages. The hyperthermophilic archaeon Sulfolobus solfataricus provides an attractive model for biochemical study of DNA replication.
Iain G, Duggin, Stephen D, Bell
openaire   +2 more sources

CRISPR-mediated gene silencing reveals involvement of the archaeal S-layer in cell division and virus infection

open access: yesNature Communications, 2019
The S-layer is a proteinaceous envelope often found in bacterial and archaeal cells. Here, the authors use CRISPR-based technology to silence slaB, encoding the S-layer membrane anchor, to show that an intact S-layer is important for cell division and ...
Isabelle Anna Zink   +5 more
doaj   +1 more source

Redox stress proteins are involved in adaptation response of the hyperthermoacidophilic archaeon Sulfolobus solfataricus to nickel challenge

open access: yesMicrobial Cell Factories, 2007
Background Exposure to nickel (Ni) and its chemical derivatives has been associated with severe health effects in human. On the contrary, poor knowledge has been acquired on target physiological processes or molecular mechanisms of this metal in model ...
Scaloni Andrea   +6 more
doaj   +1 more source

Biochemical, transcriptional and translational evidences of the phenol-meta-degradation pathway by the hyperthermophilic Sulfolobus solfataricus 98/2. [PDF]

open access: yesPLoS ONE, 2013
Phenol is a widespread pollutant and a model molecule to study the biodegradation of monoaromatic compounds. After a first oxidation step leading to catechol in mesophilic and thermophilic microorganisms, two main routes have been identified depending on
Alexia Comte   +7 more
doaj   +1 more source

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