Results 51 to 60 of about 7,405 (197)
The Chromosome Replication Machinery of the Archaeon Sulfolobus solfataricus [PDF]
Journal of Biological Chemistry, 2006 In the three domains of life, the archaea, bacteria, and eukarya, there are two general lineages of DNA replication proteins: the bacterial and the eukaryal/archaeal lineages. The hyperthermophilic archaeon Sulfolobus solfataricus provides an attractive model for biochemical study of DNA replication.
Iain G, Duggin, Stephen D, Bell
openaire +2 more sources
Intrinsic sequence specificity of the Cas1 integrase directs new spacer acquisition
eLife, 2015 The adaptive prokaryotic immune system CRISPR-Cas provides RNA-mediated protection from invading genetic elements. The fundamental basis of the system is the ability to capture small pieces of foreign DNA for incorporation into the genome at the CRISPR ...
Clare Rollie +4 more
doaj +1 more source
Nature Communications, 2019 The S-layer is a proteinaceous envelope often found in bacterial and archaeal cells. Here, the authors use CRISPR-based technology to silence slaB, encoding the S-layer membrane anchor, to show that an intact S-layer is important for cell division and ...
Isabelle Anna Zink +5 more
doaj +1 more source
Microbial Cell Factories, 2007 Background Exposure to nickel (Ni) and its chemical derivatives has been associated with severe health effects in human. On the contrary, poor knowledge has been acquired on target physiological processes or molecular mechanisms of this metal in model ...
Scaloni Andrea +6 more
doaj +1 more source
The Archaeal Elongation Factor EF-2 Induces the Release of aIF6 From 50S Ribosomal Subunit
Frontiers in Microbiology, 2021 The translation factor IF6 is a protein of about 25 kDa shared by the Archaea and the Eukarya but absent in Bacteria. It acts as a ribosome anti-association factor that binds to the large subunit preventing the joining to the small subunit.
Giada Lo Gullo +7 more
doaj +1 more source
Purification and properties of an ATPase from Sulfolobus solfataricus [PDF]
Archives of Biochemistry and Biophysics, 1992 A sulfite-activated ATPase isolated from Sulfolobus solfataricus had a relative molecular mass of 370,000. It was composed of three subunits whose relative molecular masses were 63,000, 48,000, and 24,000. The enzyme was inhibited by the vacuolar ATPase inhibitors nitrate and N-ethylmaleimide; 4-chloro-7-nitrobenzofurazan (NBD-Cl) was also inhibitory ...
L I, Hochstein, H, Stan-Lotter
openaire +2 more sources
Extensive lysine methylation in hyperthermophilic crenarchaea : potential implications for protein stability and recombinant enzymes [PDF]
, 2010 In eukarya and bacteria, lysine methylation is relatively rare and is catalysed by sequence-specific lysine methyltransferases that typically have only a single-protein target.
Botting, Catherine H. +7 more
core +1 more source
A Heterotrimeric PCNA in the Hyperthermophilic Archaeon Sulfolobus solfataricus [PDF]
Molecular Cell, 2003 The sliding clamp, PCNA, of the archaeon Sulfolobus solfataricus P2 is a heterotrimer of three distinct subunits (PCNA1, 2, and 3) that assembles in a defined manner. The PCNA heterotrimer, but not individual subunits, stimulates the activities of the DNA polymerase, DNA ligase I, and the flap endonuclease (FEN1) of S. solfataricus.
Dionne, Isabelle +4 more
openaire +2 more sources
, 2013 Little is known about the regulation of the DNA damage-mediated gene expression in archaea. Here we report that the addition of actinomycin D to Sulfolobus solfataricus cultures triggers the expression of the radA paralogue sso0777.
Campoy, S +5 more
core +1 more source
Metabolism of Pentose Sugars in the Hyperthermophilic Archaea Sulfolobus solfataricus and Sulfolobus acidocaldarius [PDF]
Journal of Biological Chemistry, 2010 We have previously shown that the hyperthermophilic archaeon, Sulfolobus solfataricus, catabolizes d-glucose and d-galactose to pyruvate and glyceraldehyde via a non-phosphorylative version of the Entner-Doudoroff pathway. At each step, one enzyme is active with both C6 epimers, leading to a metabolically promiscuous pathway.
Charlotte E M, Nunn +6 more
openaire +2 more sources