Identification of a system required for the functional surface localization of sugar binding proteins with class III signal peptides in Sulfolobus solfataricus [PDF]
The hyperthermophilic archaeon Sulfolobus solfataricus contains an unusual large number of sugar binding proteins that are synthesized as precursors with a class III signal peptide.
Szabó, Zalán, +15 more
core +1 more source
Stability of aspartate aminotransferase from Sulfolobus solfataricus [PDF]
Aspartate aminotransferase from Sulfolobus solfataricus (SsAspAT) is an extremely thermophilic and thermostable dimeric enzyme which retains its structure and reaches maximal activity at 100 degrees C. The structural stability of this protein was investigated by coupling isothermally and thermally induced denaturation studies to molecular modeling. Gel
M. I. Arnone +6 more
openaire +5 more sources
Crenarchaeal biofilm formation under extreme conditions. [PDF]
BACKGROUND: Biofilm formation has been studied in much detail for a variety of bacterial species, as it plays a major role in the pathogenicity of bacteria.
Andrea Koerdt +4 more
doaj +1 more source
The Sulfolobus solfataricus P2 genome project
Over 800 kbp of the 3‐Mbp genome of Sulfolobus solfataricus have been sequenced to date. Our approach is to sequence subclones of mapped cosmids, followed by sequencing directly on cosmid templates with custom primers. Using a prototype automated system for genome‐scale analysis, known as MAGPIE, along with other tools, we have discovered one open ...
Charlebois, RL +4 more
openaire +5 more sources
Ultrastructural and proteomic evidence for the presence of a putative nucleolus in an Archaeon
Nucleoli are subcellular compartments where transcription and maturation of pre-ribosomal RNAs occur. While the transcription of ribosomal RNAs is common to all living cells, the presence and ultrastructure of nucleoli has been only documented in ...
Parsifal F. Islas-Morales +11 more
doaj +1 more source
A triple mutant of NADP(H)-dependent malate dehydrogenase from thermotolerant Thermococcus kodakarensis has an altered cofactor preference for NAD+, as well as improved malate production compared to wildtype malate dehydrogenase.
Ravneet Mandair +2 more
doaj +1 more source
Peptidyl-tRNA hydrolase from Sulfolobus solfataricus [PDF]
An enzyme capable of liberating functional tRNA(Lys) from Escherichia coli diacetyl-lysyl-tRNA(Lys) was purified from the archae Sulfolobus solfataricus. Contrasting with the specificity of peptidyl- tRNA hydrolase (PTH) from E.coli, the S.solfataricus enzyme readily accepts E.coli formyl-methionyl-tRNA(fMet) as a substrate.
Fromant, Michel +3 more
openaire +3 more sources
The transcript cleavage factor paralogue TFS4 is a potent RNA polymerase inhibitor
Transcript cleavage factors such as eukaryotic TFIIS assist the resumption of transcription following RNA pol II backtracking. Here the authors find that one of the Sulfolobus solfataricus TFIIS homolog—TFS4—has evolved into a potent RNA polymerase ...
Thomas Fouqueau +5 more
doaj +1 more source
A genetic study of SSV1, the prototypical fusellovirus.
Viruses of thermophilic Archaea are unique in both their structures and genomic sequences. The most widespread and arguably best studied are the lemon-shaped fuselloviruses.
Eric eIverson, Kenneth eStedman
doaj +1 more source
Thermodynamics of the ATPase Cycle of GlcV, the Nucleotide-Binding Domain of the Glucose ABC Transporter of Sulfolobus solfataricus [PDF]
ATP-binding cassette transporters drive the transport of substrates across the membrane by the hydrolysis of ATP. They typically have a conserved domain structure with two membrane-spanning domains that form the transport channel and two cytosolic ...
Schuurman-Wolters, Gea, +14 more
core +1 more source

