Results 11 to 20 of about 13,977 (211)
Anti-CRISPR-Based and CRISPR-Based Genome Editing of Sulfolobus islandicus Rod-Shaped Virus 2
Viruses, 2018 Genetic engineering of viruses has generally been challenging. This is also true for archaeal rod-shaped viruses, which carry linear double-stranded DNA genomes with hairpin ends.
David Mayo-Muñoz +5 more
doaj +2 more sources
Differentiation and Structure in Sulfolobus islandicus Rod-Shaped Virus Populations
Viruses, 2017 In the past decade, molecular surveys of viral diversity have revealed that viruses are the most diverse and abundant biological entities on Earth. In culture, however, most viral isolates that infect microbes are represented by a few variants isolated ...
Maria A. Bautista +3 more
doaj +2 more sources
Archaeal GPN-loop GTPases involve a lock-switch-rock mechanism for GTP hydrolysis
mBio, 2023 Three GPN-loop GTPases, GPN1–GPN3, are central to the maturation and trafficking of eukaryotic RNA polymerase II. This GTPase family is widely represented in archaea but typically occurs as single paralogs.
Lukas Korf +9 more
doaj +1 more source
Chromosome organization affects genome evolution in Sulfolobus archaea
Nature Microbiology, 2022 In all organisms, the DNA sequence and the structural organization of chromosomes affect gene expression. The extremely thermophilic crenarchaeon Sulfolobus has one circular chromosome with three origins of replication.
Catherine Badel +2 more
semanticscholar +1 more source
Frontiers in Microbiology, 2021 Significant technical challenges have limited the study of extremophile cell biology. Here we describe a system for imaging samples at 75°C using high numerical aperture, oil-immersion lenses.
Arthur Charles-Orszag +2 more
semanticscholar +1 more source
ChemistryOpen, 2021 Due to their special chemical structure, tetraether lipids (TEL) represent essential elements of archaeal membranes, providing these organisms with extraordinary properties.
Dr. Alexander Scholte +7 more
doaj +1 more source
Chromosome conformation capture assay combined with biotin enrichment for hyperthermophilic archaea
STAR Protocols, 2021 Summary: Chromosome organization in archaea has long been enigmatic due, in part, to the typically small cell size of archaea and the extremophilic nature of many of the model archaeal species studies, rendering live-cell imaging technically challenging.
Naomichi Takemata, Stephen D. Bell
doaj +1 more source
STAR Protocols, 2021 Summary: Type III CRISPR-cas systems initiate cyclic oligo-adenylate (cOA) signaling to initiate immune response. Previously, we identified that a membrane-associated DHH-DHHA1 family protein from Sulfolobus islandicus efficiently degrades cOA.
Ruiliang Zhao +3 more
doaj +1 more source
High-resolution analysis of chromosome conformation in hyperthermophilic archaea
STAR Protocols, 2021 Summary: Chromosome conformation capture (3C) techniques are emerging as promising approaches to study genome organization in Archaea, the least understood domain of life in terms of chromosome biology. Here, we describe a 3C technique combined with deep
Naomichi Takemata, Stephen D. Bell
doaj +1 more source
The influence of the specific growth rate on the lipid composition of Sulfolobus acidocaldarius
Extremophiles, 2020 Archaeal lipids are constituted of two isoprenoid chains connected via ether bonds to glycerol in the sn-2, 3 position. Due to these unique properties archaeal lipids are significantly more stable against high temperature, low pH, oxidation and enzymatic
Julian Quehenberger +3 more
semanticscholar +1 more source