Results 11 to 20 of about 21,408 (208)

Expression, purification, and characterization of a membrane-associated cyclic oligo-adenylate degrader from Sulfolobus islandicus

open access: yesSTAR Protocols, 2021
Summary: Type III CRISPR-cas systems initiate cyclic oligo-adenylate (cOA) signaling to initiate immune response. Previously, we identified that a membrane-associated DHH-DHHA1 family protein from Sulfolobus islandicus efficiently degrades cOA.
Ruiliang Zhao   +3 more
doaj   +1 more source

High-resolution analysis of chromosome conformation in hyperthermophilic archaea

open access: yesSTAR Protocols, 2021
Summary: Chromosome conformation capture (3C) techniques are emerging as promising approaches to study genome organization in Archaea, the least understood domain of life in terms of chromosome biology. Here, we describe a 3C technique combined with deep
Naomichi Takemata, Stephen D. Bell
doaj   +1 more source

Identification of a system required for the functional surface localization of sugar binding proteins with class III signal peptides in Sulfolobus solfataricus [PDF]

open access: yes, 2007
The hyperthermophilic archaeon Sulfolobus solfataricus contains an unusual large number of sugar binding proteins that are synthesized as precursors with a class III signal peptide.
Albers S.V.   +12 more
core   +9 more sources

Effect of the Availability of the Source of Nitrogen and Phosphorus in the Bio-Oxidation of H2S by Sulfolobus metallicus

open access: yesFermentation, 2023
The effect of nitrogen and phosphorus availability on the growth of Sulfolobus metallicus was analyzed. This archaeon was subjected to a series of nitrogen and phosphorus limitation conditions to determine their effects on growth.
Javier Silva   +3 more
doaj   +1 more source

Biosynthesis of caldariellaquinone in Sulfolobus spp [PDF]

open access: yesJournal of Bacteriology, 1989
The biosynthesis of caldariellaquionone (CQ) was studied in species of Sulfolobus by measuring the incorporation of stable isotopically labeled tyrosines into CQ. By feeding a series of tyrosines labeled with deuterium or 13C and then measuring the extent and position at which label was incorporated into CQ by mass spectrometry, it was shown that more ...
D, Zhou, R H, White
openaire   +2 more sources

Species-Specific Recognition of Sulfolobales Mediated by UV-Inducible Pili and S-Layer Glycosylation Patterns

open access: yesmBio, 2020
The UV-inducible pili system of Sulfolobales (Ups) mediates the formation of species-specific cellular aggregates. Within these aggregates, cells exchange DNA to repair DNA double-strand breaks via homologous recombination. Substitution of the Sulfolobus
Marleen van Wolferen   +8 more
doaj   +1 more source

Biochemical characterization of a structure-specific resolving enzyme from Sulfolobus islandicus rod-shaped virus 2. [PDF]

open access: yesPLoS ONE, 2011
Sulfolobus islandicus rod shaped virus 2 (SIRV2) infects the archaeon Sulfolobus islandicus at extreme temperature (70°C-80°C) and acidity (pH 3). SIRV2 encodes a Holliday junction resolving enzyme (SIRV2 Hjr) that has been proposed as a key enzyme in ...
Andrew F Gardner   +2 more
doaj   +1 more source

Snapshots of Motion: A Novel Structural Intermediate Reveals Conserved Dynamics in Archaeal DNA Ligases. [PDF]

open access: yesProteins
ABSTRACT We present the first x‐ray crystallographic structural evidence of an archaeal DNA ligase showing the AMP covalent adduct together with further cofactor hydrolysis, capturing a transient intermediary in the first step of the ligation reaction, triggered by the pyrophosphate hydrolysis.
Quintana-Armas AX   +3 more
europepmc   +2 more sources

Hot and crispy : CRISPR-Cas systems in the hyperthermophile Sulfolobus solfataricus [PDF]

open access: yes, 2013
The CRISPR (clustered regularly interspaced short palindromic repeats) and Cas (CRISPR-associated) genes are widely spread in bacteria and archaea, representing an intracellular defence system against invading viruses and plasmids.
White, Malcolm F., Zhang, Jing
core   +2 more sources

Differential Virus Host-Ranges of the Fuselloviridae of Hyperthermophilic Archaea: Implications for Evolution in Extreme Environments

open access: yesFrontiers in Microbiology, 2012
An emerging model for investigating virus-host interactions in hyperthermophilic Archaea is the Fusellovirus-Sulfolobus system. The host, Sulfolobus, is a hyperthermophilic acidophile endemic to sulfuric volcanic-driven hot springs worldwide.
Ruben Michael eCeballos   +5 more
doaj   +1 more source

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