Machine Learning Uncovers a Data-Driven Transcriptional Regulatory Network for the Crenarchaeal Thermoacidophile Sulfolobus acidocaldarius [PDF]
Frontiers in Microbiology, 2021 Dynamic cellular responses to environmental constraints are coordinated by the transcriptional regulatory network (TRN), which modulates gene expression.
Siddharth M. Chauhan +9 more
doaj +2 more sources
5′-untranslated region sequences enhance plasmid-based protein production in Sulfolobus acidocaldarius [PDF]
Frontiers in MicrobiologySulfolobus acidocaldarius, a thermoacidophilic archaeon of the phylum Thermoproteota (former Crenarchaeota), is a widely used model organism for gene deletion studies and recombinant protein production.
Laura Kuschmierz +6 more
doaj +2 more sources
DNA-Binding Properties of a Novel Crenarchaeal Chromatin-Organizing Protein in Sulfolobus acidocaldarius [PDF]
Biomolecules, 2022 In archaeal microorganisms, the compaction and organization of the chromosome into a dynamic but condensed structure is mediated by diverse chromatin-organizing proteins in a lineage-specific manner. While many archaea employ eukaryotic-type histones for
Liesbeth Lemmens +7 more
doaj +2 more sources
DNA Polymerase B1 Binding Protein 1 Is Important for DNA Repair by Holoenzyme PolB1 in the Extremely Thermophilic Crenarchaeon Sulfolobus acidocaldarius [PDF]
Microorganisms, 2021 DNA polymerase B1 (PolB1) is a member of the B-family DNA polymerase family and is a replicative DNA polymerase in Crenarchaea. PolB1 is responsible for the DNA replication of both the leading and lagging strands in the thermophilic crenarchaeon ...
Hiroka Miyabayashi +2 more
doaj +2 more sources
Identification of the Genes Related to the Glycogen Metabolism in Hyperthermophilic Archaeon, Sulfolobus acidocaldarius [PDF]
Frontiers in Microbiology, 2021 Glycogen is a polysaccharide that comprises α-1,4-linked glucose backbone and α-1,6-linked glucose polymers at the branching points. It is widely found in organisms ranging from bacteria to eukaryotes.
Areum Lee +5 more
doaj +2 more sources
Versatile genetic tool box for the crenarchaeote Sulfolobus acidocaldarius [PDF]
Frontiers in Microbiology, 2012 For reverse genetic approaches inactivation or selective modification of genes are required to elucidate their putative function. Sulfolobus acidocaldarius is a thermoacidophilic Crenarchaeon which grows optimally at 76 °C and pH 3.
Michaela eWagner +6 more
doaj +6 more sources
A TetR-family transcription factor regulates fatty acid metabolism in the archaeal model organism Sulfolobus acidocaldarius [PDF]
Nature Communications, 2019 Certain archaea appear to metabolize fatty acids, but the regulation of these pathways is unclear. Here, Wang et al. provide genetic, functional and structural evidence supporting that a TetR-family transcriptional regulator is involved in regulation of ...
Kun Wang +11 more
doaj +2 more sources
PolB1 Is Sufficient for DNA Replication and Repair Under Normal Growth Conditions in the Extremely Thermophilic Crenarchaeon Sulfolobus acidocaldarius [PDF]
Frontiers in Microbiology, 2020 The thermophilic crenarchaeon Sulfolobus acidocaldarius has four DNA polymerases (DNAPs): PolB1, PolB2, PolB3, and Dbh (PolY). Previous in vitro studies suggested that PolB1 is the main replicative DNAP of Sulfolobales whereas PolB2 and Y-family ...
Hiroka Miyabayashi +4 more
doaj +2 more sources
Lipid hydrogen isotope compositions primarily reflect growth water in the model archaeon Sulfolobus acidocaldarius [PDF]
Applied and Environmental MicrobiologyThe stable hydrogen isotope composition (δ2H) of lipid biomarkers can track environmental processes and remain stable over geologically relevant time scales, enabling studies of past climate, hydrology, and ecology.
Carolynn M. Harris +12 more
doaj +2 more sources
The use of thermostable fluorescent proteins for live imaging in Sulfolobus acidocaldarius [PDF]
Frontiers in MicrobiologyIntroductionAmong hyperthermophilic organisms, in vivo protein localization is challenging due to the high growth temperatures that can disrupt proper folding and function of mostly mesophilic-derived fluorescent proteins.
Alejandra Recalde +6 more
doaj +2 more sources