Identification of XylR, the Activator of Arabinose/Xylose Inducible Regulon in Sulfolobus acidocaldarius and Its Application for Homologous Protein Expression [PDF]
Frontiers in Microbiology, 2020 The thermophilic archaeon Sulfolobus acidocaldarius can use different carbon sources for growth, including the pentoses D-xylose and L-arabinose. In this study, we identified the activator XylR (saci_2116) responsible for the transcriptional regulation ...
Nienke van der Kolk +7 more
doaj +4 more sources
Early Response of Sulfolobus acidocaldarius to Nutrient Limitation [PDF]
Frontiers in Microbiology, 2019 In natural environments microorganisms encounter extreme changes in temperature, pH, osmolarities and nutrient availability. The stress response of many bacterial species has been described in detail, however, knowledge in Archaea is limited.
Lisa F. Bischof +16 more
doaj +13 more sources
Physiological Characterization of Sulfolobus acidocaldarius in a Controlled Bioreactor Environment. [PDF]
Int J Environ Res Public Health, 2021 The crenarchaeal model organism Sulfolobus acidocaldarius is typically cultivated in shake flasks. Although shake flasks represent the state-of-the-art for the cultivation of this microorganism, in these systems crucial process parameters, like pH or ...
Rastädter K +3 more
europepmc +6 more sources
Engineering of Sulfolobus acidocaldarius for Hemicellulosic Biomass Utilization. [PDF]
J Microbiol Biotechnol, 2022 The saccharification of cellulose and hemicellulose is essential for utilizing lignocellulosic biomass as a biofuel. While cellulose is composed of glucose only, hemicelluloses are composed of diverse sugars such as xylose, arabinose, glucose, and ...
Lee A, Jin H, Cha J.
europepmc +4 more sources
Machine Learning Uncovers a Data-Driven Transcriptional Regulatory Network for the Crenarchaeal Thermoacidophile Sulfolobus acidocaldarius [PDF]
Frontiers in Microbiology, 2021 Dynamic cellular responses to environmental constraints are coordinated by the transcriptional regulatory network (TRN), which modulates gene expression.
Siddharth M. Chauhan +9 more
doaj +3 more sources
Translesion synthesis of apurinic/apyrimidic siteanalogues by Y-family DNA polymerase Dbh fromSulfolobus acidocaldarius [PDF]
Acta Biochimica et Biophysica Sinica, 2022 Apurinic/apyrimidic (AP) sites are severe DNA damages and strongly block DNA extension by major DNA polymerases. Y-family DNA polymerases possess a strong ability to bypass AP sites and continue the DNA synthesis reaction, which is called translesion ...
Wang Weiwei +7 more
doaj +3 more sources
A web-based atlas for exploring post-transcriptional regulation in the archaeon Sulfolobus acidocaldarius [PDF]
mSystemsThe archaeal domain contains organisms that are well-adapted to extreme conditions and changes in their habitat. Post-transcriptional regulation plays a key role in environmental adaptation, including rapid molecular responses to stress conditions.
Michel Brück +3 more
doaj +3 more sources
The use of thermostable fluorescent proteins for live imaging in Sulfolobus acidocaldarius [PDF]
Frontiers in MicrobiologyIntroductionAmong hyperthermophilic organisms, in vivo protein localization is challenging due to the high growth temperatures that can disrupt proper folding and function of mostly mesophilic-derived fluorescent proteins.
Alejandra Recalde +6 more
doaj +3 more sources
DNA-Binding Properties of a Novel Crenarchaeal Chromatin-Organizing Protein in Sulfolobus acidocaldarius [PDF]
Biomolecules, 2022 In archaeal microorganisms, the compaction and organization of the chromosome into a dynamic but condensed structure is mediated by diverse chromatin-organizing proteins in a lineage-specific manner. While many archaea employ eukaryotic-type histones for
Liesbeth Lemmens +7 more
doaj +3 more sources
Versatile genetic tool box for the crenarchaeote Sulfolobus acidocaldarius [PDF]
Frontiers in Microbiology, 2012 For reverse genetic approaches inactivation or selective modification of genes are required to elucidate their putative function. Sulfolobus acidocaldarius is a thermoacidophilic Crenarchaeon which grows optimally at 76 °C and pH 3.
Michaela eWagner +6 more
doaj +8 more sources