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Recombinant protein expression in Sulfolobus islandicus
2021Since its invention, recombinant protein expression has greatly facilitated our understanding of various cellular processes in different biological systems because theoretically this technique renders any gene to be expressed in a mesophilic host like Escherichia coli, thus allowing functional characterizations of proteins of interest.
Xu, Feng, Qunxin, She
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Extremophiles, 2002
The taxonomic position of a thermoacidophilic crenarchaeote Sulfolobus sp. strain 7, previously isolated from the Beppu Hot Springs in the geothermal area of Kyushu Island, Japan, was investigated by cloning and sequencing, by phylogenetic analysis of the 16S rRNA gene sequence, by DNA-DNA homology with similar species, and by biochemical ...
Toshio Iwasaki +2 more
exaly +3 more sources
The taxonomic position of a thermoacidophilic crenarchaeote Sulfolobus sp. strain 7, previously isolated from the Beppu Hot Springs in the geothermal area of Kyushu Island, Japan, was investigated by cloning and sequencing, by phylogenetic analysis of the 16S rRNA gene sequence, by DNA-DNA homology with similar species, and by biochemical ...
Toshio Iwasaki +2 more
exaly +3 more sources
β-Glycosidase from Sulfolobus solfataricus
2001Publisher Summary The hydrolysis of glycosidic bonds is crucial for several cell functions, such as energy uptake, cell wall expansion, and turnover of signaling molecules. Because the diversity of saccharides, there is a wide variety of glycosyl-hydrolases that have been classified in more than 74 families based on amino acid sequence similarities ...
Moracci M, Ciaramella M, Rossi M
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A defined cultivation medium for Sulfolobus acidocaldarius
Journal of Biotechnology, 2019The thermoacidophilic Crenarchaeon Sulfolobus acidocaldarius is an important model organism for Archaea and genetic systems are well established. To date, the organism is routinely cultivated on complex media based on protein hydrolysates and no common defined medium is established. In this work we address this lack of a standardized defined medium and
Julian Quehenberger +5 more
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1978
Our work on Sulfolobus originally arose out of a determination to find a natural habitat for Thermoplasma. As discussed in the previous chapter, Thermoplasma was originally discovered as an acidophilic thermophile living in self-heated coal refuse piles.
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Our work on Sulfolobus originally arose out of a determination to find a natural habitat for Thermoplasma. As discussed in the previous chapter, Thermoplasma was originally discovered as an acidophilic thermophile living in self-heated coal refuse piles.
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Stringent control in the archaeal genus Sulfolobus
Research in Microbiology, 2004Six Archaea belonging to the phylum Euryarchaeota were previously analyzed with respect to stringent control. Only one of the strains studied was shown to possess Bacteria-like stringent control over stable RNA accumulation; ppGpp and pppGpp production was totally lacking in all Archaea analyzed.
CELLINI, ANDREA +6 more
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Interactions of Sulfolobus acidocaldarius with uranium
Radiochimica Acta, 2010Abstract Interactions of the acidothermophilic archaeon Sulfolobus acidocaldarius DSM 639 with U(VI) were studied by using a combination of batch experiments, X-ray absorption spectroscopy (XAS), and time-resolved laser-induced fluorescence spectroscopy (TRLFS).
Reitz, T. +3 more
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2-Propanol degradation by Sulfolobus solfataricus
Biotechnology Letters, 2003Sulfolobus solfataricus used 2-propanol and 2-propanone (acetone) when grown in static cultures at 78 degrees C with or without glucose at 10 g l-1. The presence of 3.92 g 2-propanol l-1 in both cases inhibited growth. However, acetone accumulation following 2-propanol depletion suggested that 2-propanol was co-metabolized via the acetone metabolic ...
Radianingtyas H, Wright PC
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Sequencing the Genome of Sulfolobus solfataricus P2
1998Using a simple strategy and current technology, we are sequencing the genome of the archaeon Sulfolobus solfataricus P2. The resulting data are processed through an efficient and comprehensive semi-automated bioinformatics system, minimizing the effort required to identify the features of interest.
Sensen, Christopher W +5 more
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The α-l-fucosidase from Sulfolobus solfataricus
Extremophiles, 2007Glycoside hydrolases form hyperthermophilic archaea are interesting model systems for the study of catalysis at high temperatures and, at the moment, their detailed enzymological characterization is the only approach to define their role in vivo. Family 29 of glycoside hydrolases classification groups alpha-L-fucosidases involved in a variety of ...
CobucciPonzano B +3 more
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