Results 81 to 90 of about 28,271 (292)

Quantifying Soil Microbiome Abundance by Metatranscriptomics and Complementary Molecular Techniques—Cross‐Validation and Perspectives

Molecular Ecology Resources, EarlyView.
ABSTRACT Linking meta‐omics and biogeochemistry approaches in soils has remained challenging. This study evaluates the use of an internal RNA extraction standard and its potential for making quantitative estimates of a given microbial community size (biomass) in soil metatranscriptomics. We evaluate commonly used laboratory protocols for RNA processing,
Mathilde Borg Dahl, Stella Brachmann, Andrea Söllinger, Marina Schnell, Laureen Ahlers, Magdalena Wutkowska, Katharina J. Hoff, Neetika Nath, Verena Groß, Haitao Wang, Micha Weil, Marc Piecha, Marc Schaffer, Corinna Jensen, Andreas W. Kuss, Christoph Gall, Erika Wimmer, Thomas Pribasnig, Alexander Tøsdal Tveit, Bjarni D. Sigurdsson, Christa Schleper, Andreas Richter, Tim Urich   +22 more
wiley   +1 more source

Functional assignment of multiple ESCRT‐III homologs in cell division and budding in Sulfolobus islandicus

Molecular Microbiology, 2017
The archaea Sulfolobus utilizes the ESCRT‐III‐based machinery for cell division. This machinery comprises three proteins: CdvA, Eukaryotic‐like ESCRT‐III and Vps4.
Junfeng Liu, Renxia Gao, Chen-Hong Li, J. Ni, Zhaojie Yang, Qi Zhang, Haining Chen, Yulong Shen   +7 more
semanticscholar   +1 more source

Optimization of Enzymatic Gas-Phase Reactions by Increasing the Long-Term Stability of the Catalyst [PDF]

, 2004
Enzymatic gas-phase reactions are usually performed in continuous reactors, and thus very stable and active catalysts are required to perform such transformations on cost-effective levels.
Büchs, Jochen,, Daussmann, Thomas,, Ferloni, Clara,, Heinemann, Matthias,, Hummel, Werner,   +4 more
core   +2 more sources

Isotopologue Ratios Identify 13C‐Depleted Biomarkers in Environmental Samples Impacted by Methane Turnover

Rapid Communications in Mass Spectrometry, Volume 39, Issue 22, 30 November 2025.
ABSTRACT Rationale The stable carbon isotopic composition (δ13C) of individual lipids is of great value in studying carbon cycling. Among those, microbial lipids in sediments impacted by high methane turnover stand out due to their uniquely depleted isotopic fingerprint.
Janina Groninga, Julius Lipp, Min Song, Kai‐Uwe Hinrichs   +3 more
wiley   +1 more source

The transcript cleavage factor paralogue TFS4 is a potent RNA polymerase inhibitor

Nature Communications, 2017
Transcript cleavage factors such as eukaryotic TFIIS assist the resumption of transcription following RNA pol II backtracking. Here the authors find that one of the Sulfolobus solfataricus TFIIS homolog—TFS4—has evolved into a potent RNA polymerase ...
Thomas Fouqueau, Fabian Blombach, Ross Hartman, Alan C. M. Cheung, Mark J. Young, Finn Werner   +5 more
doaj   +1 more source

A systems biology approach reveals major metabolic changes in the thermoacidophilic archaeon Sulfolobus solfataricus in response to the carbon source L‐fucose versus D‐glucose

Molecular Microbiology, 2016
Archaea are characterised by a complex metabolism with many unique enzymes that differ from their bacterial and eukaryotic counterparts. The thermoacidophilic archaeon Sulfolobus solfataricus is known for its metabolic versatility and is able to utilize ...
Jacqueline Wolf, Helge Stark, Katharina Fafenrot, A. Albersmeier, T. K. Pham, Katrin B. Müller, B. Meyer, L. Hoffmann, Lu Shen, Stefan P. Albaum, T. Kouril, Kerstin Schmidt-Hohagen, M. Neumann-Schaal, C. Bräsen, J. Kalinowski, P. Wright, S. Albers, D. Schomburg, B. Siebers   +18 more
semanticscholar   +1 more source

Microbial diversity and function in the rhizosphere microbiome: Driving forces and monitoring approaches

Agrosystems, Geosciences &Environment, Volume 8, Issue 3, September 2025.
Abstract As an ecological unit, the rhizosphere microbiome preserves an enormous and largely unexplored diversity of microbes that play essential roles in plant health and soil functions. Omics technologies encompassing high‐throughput approaches such as metagenomics, metatranscriptomics, and metaproteomics provide helpful tools to unravel the ...
Chinenyenwa Fortune Chukwuneme, Olubukola Oluranti Babalola   +1 more
wiley   +1 more source

Structure of Csx1-cOA4 complex reveals the basis of RNA decay in Type III-B CRISPR-Cas

Nature Communications, 2019
Type III CRISPR-Cas RNases from the Csm and Csx families are activated by cyclic oligoadenylates (cOA). Here the authors present the cOA bound Sulfolobus islandicus Csx1 structure, which forms a hexamer and reveal an allosteric mechanism for the ...
Rafael Molina, Stefano Stella, Mingxia Feng, Nicholas Sofos, Vykintas Jauniskis, Irina Pozdnyakova, Blanca López-Méndez, Qunxin She, Guillermo Montoya   +8 more
doaj   +1 more source

Harnessing hyperthermostable lactonase from Sulfolobus solfataricus for biotechnological applications

Scientific Reports, 2016
Extremozymes have gained considerable interest as they could meet industrial requirements. Among these, SsoPox is a hyperthermostable enzyme isolated from the archaeon Sulfolobus solfataricus.
B. Rémy, Laure Plener, L. Poirier, M. Elias, D. Daudé, E. Chabrière   +5 more
semanticscholar   +1 more source

hSSB1 interacts directly with the MRN complex stimulating its recruitment to DNA double-strand breaks and its endo-nuclease activity [PDF]

, 2013
hSSB1 is a recently discovered single-stranded DNA binding protein that is essential for efficient repair of DNA double-strand breaks (DSBs) by the homologous recombination pathway.
Bain, Amanda, Bolderson, Emma, Cubeddu, Liza, Khanna, Kum Kum, Menon, Dinoop, Richard, Derek J., Urquhart, Aaron J., White, Malcolm F.   +7 more
core   +1 more source