Results 121 to 130 of about 14,198 (193)

Isolation of extracellular polymeric substances from biofilms of the thermoacidophilic archaeon Sulfolobus acidocaldarius

Frontiers in Bioengineering and Biotechnology, 2015
Extracellular polymeric substances (EPS) are the major structural and functional components of microbial biofilms. The aim of this study was to establish a method for EPS isolation from biofilms of the thermoacidophilic archaeon Sulfolobus acidocaldarius
Silke eJachlewski, Witold D. eJachlewski, Uwe eLinne, Christopher eBräsen, Jost eWingender, Bettina eSiebers   +5 more
doaj   +1 more source

Towards a molecular picture of the archaeal cell surface

Nature Communications
Archaea produce various protein filaments with specialised functions. While some archaea produce only one type of filament, the archaeal model species Sulfolobus acidocaldarius generates four.
Matthew C. Gaines, Michail N. Isupov, Mathew McLaren, Clara L. Mollat, Risat Ul Haque, Jake K. Stephenson, Shamphavi Sivabalasarma, Cyril Hanus, Daniel Kattnig, Vicki A. M. Gold, Sonja Albers, Bertram Daum   +11 more
doaj   +1 more source

Generation of proton-motive force by an archaeal terminal quinol oxidase from Sulfolobus acidocaldarius [PDF]

, 1994
The terminal quinol oxidase of the cytochrome aa3 type was isolated from the extreme thermo-acidophilic archaeon Sulfolobus acidocaldarius. In micellar solution, the enzyme oxidized various quinols and exerted the highest activity with the physiological ...
Konings, Wilhelmus, Schäfer, Günter, Gleissner, Michael, Elferink, Maria, Driessen, Arnold J.M., Anemüller, Stefan   +5 more
core   +1 more source

Energy flux controls tetraether lipid cyclization in Sulfolobus acidocaldarius

bioRxiv, 2019
Microorganisms regulate the composition of their membranes in response to environmental cues. Many archaea maintain the fluidity and permeability of their membranes by adjusting the number of cyclic moieties within the cores of their glycerol ...
A. Zhou, Y. Weber, B. Chiu, F. Elling, A. Cobban, A. Pearson, W. Leavitt   +6 more
semanticscholar   +1 more source

Development of the Multiple Gene Knockout System with One-Step PCR in Thermoacidophilic Crenarchaeon Sulfolobus acidocaldarius [PDF]

, 2017
Multiple gene knockout systems developed in the thermoacidophilic crenarchaeon Sulfolobus acidocaldarius are powerful genetic tools. However, plasmid construction typically requires several steps.
Norio Kurosawa, Shoji Suzuki
core   +1 more source

Improved Isolation of SlaA and SlaB S-layer proteins in Sulfolobus acidocaldarius

, 2020
International audienceThe Sulfolobus acidocaldarius S-layer is composed of two main proteins: SlaA, which forms the ordered structure of the S-layer matrix, and SlaB, which supports and anchors the S-layer into the tetraether lipid membrane.
Simonin, Pierre, Lombard, Carine, Kish, Adrienne, Huguet, Arnaud   +3 more
core   +1 more source

Reconstitution of the Leucine Transport System of Lactococcus lactis into Liposomes Composed of Membrane-Spanning Lipids from Sulfolobus acidocaldarius

, 1992
The effect of bipolar tetraether lipids, extracted from the thermophilic archaebacterium Sulfolobus acidocaldarius, on the branched-chain amino acid transport system of the mesophilic bacterium Lactococcus lactis was investigated. Liposomes were prepared
Konings, Wilhelmus, Elferink, Maria, Driessen, Arnold J.M., in t Veld, Geertruida   +3 more
core   +1 more source

Evolutionary conservation of ubiquitin-like protein urmylation as revealed by URM1 gene shuffle from archaea to yeast

Communications Biology
Urm1 from yeast is a unique ubiquitin-like protein with dual functionality. It has been shown to operate in tRNA thiolation and protein urmylation, combining features typical of bacterial sulfur carriers and classical ubiquitin-like modifiers.
Katharina Zupfer, Lars Kaduhr, Larissa Bessler, Mark Helm, Raffael Schaffrath   +4 more
doaj   +1 more source

Crystal structure of Sulfolobus acidocaldarius aspartate carbamoyltransferase in complex with its allosteric activator CTP

, 2008
Aspartate carbamoyltransferase (ATCase) is a paradigm for allosteric regulation of enzyme activity. B-class ATCases display very similar homotropic allosteric behaviour, but differ extensively in their heterotropic patterns.
De Vos, Dirk, Van Beeumen, Jozef, Xu, Ying, Van Petegem, Filip, De Vos, Danièle, Aerts, Tony   +5 more
core   +1 more source

Using coffee waste as feedstock for continuous cultivation of Sulfolobus acidocaldarius.

Bioresource Technology
Sulfolobus acidocaldarius is a thermoacidophilic archaeon with high potential for robust continuous bioprocesses under extreme conditions. In this study, a continuous cultivation process using spent coffee grounds (SCGs) as a lignocellulosic feedstock ...
C. Rausch, S. Kittler, P. Mayer, M. Schittmayer, Oliver Spadiut   +4 more
semanticscholar   +1 more source