Results 111 to 120 of about 14,198 (193)
Designing Enzymatic Reactivity with an Expanded Palette
ChemBioChem, Volume 26, Issue 11, June 3, 2025.Innovation in biocatalysis is rapidly increasingly the diversity of catalytic reactivity that can be mediated by enzymes, addressing a key bottleneck for their widespread adoption in industrial chemical synthesis. A key approach to this is building enzymes with unnatural catalytic components that provide an expanded palette with new possibilities for ...
Reuben B. Leveson‐Gower
wiley +1 more source
Expanding and understanding the genetic toolbox of the hyperthermophilic genus Sulfolobus [PDF]
, 2009 Although Sulfolobus species are among the best studied archaeal micro-organisms, the development and availability of genetic tools has lagged behind. In the present paper, we discuss the latest progress in understanding recombination events of exogenous ...
Ajon, M. +4 more
core +2 more sources
ChemBioChem, Volume 26, Issue 11, June 3, 2025.An affitin derived from the Sac7d scaffold enables the light‐controlled affinity purification of antibodies under physiological conditions, thus overcoming limitations of protein A chromatography. X‐ray crystallographic analysis and affinity measurements reveal complex formation with Fc at the CH2/CH3 junction, similar to protein A/G and FnRn. Combined
Felix Veitl +7 more
wiley +1 more source
Mass Spectrometry Reviews, Volume 44, Issue 3, Page 213-453, May/June 2025.Abstract The use of matrix‐assisted laser desorption/ionization (MALDI) mass spectrometry for the analysis of carbohydrates and glycoconjugates is a well‐established technique and this review is the 12th update of the original article published in 1999 and brings coverage of the literature to the end of 2022.
David J. Harvey
wiley +1 more source
Discovering Hidden Archaeal and Bacterial Lipid Producers in a Euxinic Marine System
Environmental Microbiology, Volume 27, Issue 3, March 2025.We investigated the bacterial and archaeal membrane lipids in the Black Sea, focusing on isoprenoidal and branched GDGTs, combining high‐resolution accurate mass/mass spectrometry with metagenomics. Our objective was to identify the potential biological producers of these lipids and to begin understanding their role in microbial adaptation.
Dina Castillo Boukhchtaber +6 more
wiley +1 more source
Scientific Reports, 2018 Ketol-acid reductoisomerase (KARI) is a bifunctional enzyme in the second step of branched-chain amino acids biosynthetic pathway. Most KARIs prefer NADPH as a cofactor.
Chin-Yu Chen +6 more
doaj +1 more source
ESCRT-III mediated cell division in Sulfolobus acidocaldarius –A reconstitution perspective
Frontiers in Microbiology, 2014 In the framework of Synthetic Biology, it has become an intriguing question what would be the minimal representation of cell division machinery. Thus, it seems appropriate to compare how cell division is realized in different microorganisms.
Tobias eHärtel, Petra eSchwille
doaj +1 more source
Inducible and constitutive promoters for genetic systems in Sulfolobus acidocaldarius [PDF]
, 2018 Central to genetic work in any organism are the availability of a range of inducible and constitutive promoters. In this work we studied several promoters for use in the hyperthermophilic archaeon Sulfolobus acidocaldarius. The promoters were tested with
Berkner, Silvia +3 more
core
Data_Sheet_1_Early Response of Sulfolobus acidocaldarius to Nutrient Limitation.PDF
, 2019 In natural environments microorganisms encounter extreme changes in temperature, pH, osmolarities and nutrient availability. The stress response of many bacterial species has been described in detail, however, knowledge in Archaea is limited.
Jörn Kalinowski (23807) +14 more
core +1 more source