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The enzymatic sulphation of heparan sulphate by hen's uterus
Biochimica et Biophysica Acta (BBA) - General Subjects, 1973Abstract A sulphotransferase preparation from hen's uterus catalysed the transfer of sulphate from adenosine 3′-phosphate 5′-sulphatophosphate to N-desulphated heparan sulphate, heparan sulphate, N-desulphated heparin and dermatan sulphate. Heparin, chondroitin sulphate and hyaluronic acid were inactive as substrates for the enzyme.
Alan H. Johnson, John R. Baker
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The functions of sulphated monosaccharides within glycosaminoglycans (GAGs) and glycoproteins are being studied intensely, but progress is hindered by an inability to selectively desulphate glycoconjugates. We recently identified an N-acetylglucosamine-6-sulphate sulphatase (NG6SS) from bovine kidney that can remove sulphate from N-acetylglucosamine-6 ...
Richard D. Cummings, Ali Shilatifard
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Heparan sulphates, the N-sulphated polysaccharides components of proteoglycans, are common constituents of cell surfaces and the extracellular matrix. The heparan sulphate polysaccharide chain has a unique molecular design in the which the clusters of N- and O-sulphated sugar residues, separated by regions of low sulphation, determine specific protein ...
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The nature of the sulphate grouping in a rat salivary sulphated glycoprotein
Archives of Oral Biology, 1976Abstract A 35S-labelled sulphated glycoprotein was isolated from rat whole saliva. Stability studies on the sulphate residues present in the intact glycoprotein in 0.25 M HCl gave a half-life of 71 min. Partial acid hydrolysis of the 35S-labelled glycoprotein in 0.1 M HCl for 90 min at 100 °C liberated a radioactive component which was free from ...
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