Results 181 to 190 of about 1,097,727 (354)

Rapid screening of staphylokinase protein variants using an unpurified cell‐free expression system

FEBS Open Bio, EarlyView.
An unpurified cell‐free protein synthesis (CFPS) platform enables rapid functional screening of staphylokinase variants. Direct plasminogen‐activation assays performed in microplate format provide real‐time activity readouts, allowing rapid identification and ranking of variants with improved or reduced fibrinolytic activity without protein ...
Maria Tomková, Veronika Hovanová, Jiri Damborsky, Erik Sedlák   +3 more
wiley   +1 more source

Evolutionarily divergent DUF4465 domains have a common vitamin B12‐binding function

FEBS Open Bio, EarlyView.
We show that DUF4465 family proteins, widespread across bacteria from gut microbiomes, hydrothermal vents, and soil, share a common vitamin B12‐binding function. These augmented β‐jellyroll proteins bind vitamin B12 via extended loops. Our findings establish sequence‐diverse DUF4465 proteins as a widespread class of B12‐binding proteins, highlighting ...
Charlea Clarke, Michal Banasik, Rokas Juodeikis, Martin J. Warren, Richard W. Pickersgill   +4 more
wiley   +1 more source

Electrochemical Surface Composition of Iridium Oxide IrO₂(110): Implications for the Oxygen Evolution Reaction Mechanism. [PDF]

ACS Omega
Arevalo RL, Gueriba JS, Nakanishi H.
europepmc   +1 more source

Large‐scale bidirectional arrayed genetic screens identify OXR1 and EMC4 as modifiers of αSynuclein aggregation

FEBS Open Bio, EarlyView.
Activation of the mitochondrial protein OXR1 increases pSyn129 αSynuclein aggregation by lowering ATP levels and altering mitochondrial membrane potential, particularly in response to MSA‐derived fibrils. In contrast, ablation of the ER protein EMC4 enhances autophagic flux and lysosomal clearance, broadly reducing α‐synuclein aggregates.
Sandesh Neupane, Lea Nikolić, Lorenzo Maraio, Thomas Goiran, Nathan Karpilovsky, Stefano Sellitto, Vangelis Bouris, Jiang‐An Yin, Ronald Melki, Edward A Fon, Adriano Aguzzi, Elena De Cecco   +11 more
wiley   +1 more source

Influence of Multiple Castings and Surface Treatments on Metal-Ceramic Bond Strength and Surface Composition Variations of Nickel-Chromium (Ni-Cr) Alloy. [PDF]

Cureus
Chanu LB, Dhal A, Patro TK, Garhnayak L, Kumar U, Tasar D.   +5 more
europepmc   +1 more source

Suppression of lung adenocarcinoma migration through organelle alkalization by human lactoferrin – albumin fusion

FEBS Open Bio, EarlyView.
This paper reveals how human lactoferrin–albumin fusion (hLF‐HSA) potently suppresses lung adenocarcinoma cell migration. hLF‐HSA upregulates NHE7, leading to Golgi alkalization, disruption of the Golgi secretome, downregulation of MMP1, and reversal of EMT. These findings suggest a novel Golgi‐targeting strategy to suppress cancer cell migration.
Hana Nopia, Masahiro Kimura, Daisuke Kurimoto, Atsushi Sato   +3 more
wiley   +1 more source

Surface Composition Impacts Selectivity of ZnTe Photocathodes in Photoelectrochemical CO₂ Reduction Reaction. [PDF]

ACS Energy Lett
Zeng G, Liu G, Liu G, Panzeri G, Kim C, Song C, Alley OJ, Bell AT, Weber AZ, Toma FM.   +9 more
europepmc   +1 more source

Long‐term hippocampal alterations and cognitive impairment in a murine model of surgical sepsis

FEBS Open Bio, EarlyView.
Using a mouse model of surgical sepsis, we tested long‐term memory and analyzed the transcriptome of single cells isolated from the hippocampus. Survivor mice showed worse memory, loss of certain brain cell subpopulations, and abnormal immune cell activity—suggesting that post‐sepsis brain alterations may be linked to cognitive deficits.
Dong Seong Cho, Rebecca Schmitt, Aneesha Dasgupta, Alexandra Ducharme, Jason Doles   +4 more
wiley   +1 more source

Activated Carbon-Supported Pt Catalysts Intended for the Hydroprocessing of Lipid Feedstocks: Effects of Support Surface Composition and Impregnation Protocol. [PDF]

Molecules
Brandão RD, de Freitas Júnior AM, Linares JJ, Suarez PAZ, Dutra RC, Garnier J, Tonhá MS, Ballesteros-Plata D, Rodríguez-Castellón E, Prauchner MJ.   +9 more
europepmc   +1 more source

VLT/SINFONI OBSERVATIONS OF EUROPA: NEW INSIGHTS INTO THE SURFACE COMPOSITION

, 2016
N. Ligier, F. Poulet, J. Carter, R. Brunetto, F. Gourgeot   +4 more
semanticscholar   +1 more source