Results 131 to 140 of about 654,094 (303)

Mycobacterial cell division arrest and smooth‐to‐rough envelope transition using CRISPRi‐mediated genetic repression systems

FEBS Open Bio, EarlyView.
CRISPRI‐mediated gene silencing and phenotypic exploration in nontuberculous mycobacteria. In this Research Protocol, we describe approaches to control, monitor, and quantitatively assess CRISPRI‐mediated gene silencing in M. smegmatis and M. abscessus model organisms.
Vanessa Point, Wafaa Achache, Janïs Laudouze, Eliana Sepulveda Ramos, Mickaël Maziero, Céline Crauste, Stéphane Canaan, Pierre Santucci   +7 more
wiley   +1 more source

Comparison of Surface Current Measurement Between Compact and Square-Array Ocean Radar

Journal of Marine Science and Engineering
High-frequency (HF) ocean radars have become essential tools for monitoring surface currents, offering real-time, wide-area coverage with cost-effectiveness.
Yu-Hsuan Huang, Chia-Yan Cheng
doaj   +1 more source

Current flow in a plasma caused by dielectric breakdown [PDF]

Spacecraft with a thin dielectric coating on the outer surface of the structure which are biased (-200 V) negative relative to the atmospheric plasma are susceptible to dielectric breakdown.
Carruth, M. R., Jr., Gray, P. A., Vaughn, J. A.   +2 more
core   +1 more source

dUTPase is essential in zebrafish development and possesses several single‐nucleotide variants with pronounced structural and functional consequences

FEBS Open Bio, EarlyView.
dUTPases are involved in balancing the appropriate nucleotide pools. We showed that dUTPase is essential for normal development in zebrafish. The different zebrafish genomes contain several single‐nucleotide variations (SNPs) of the dut gene. One of the dUTPase variants displayed drastically lower protein stability and catalytic efficiency as compared ...
Viktória Perey‐Simon, Angéla Békesi, Latifa Kazzazy, Jázmin Mihály, Máté Varga, Beáta G. Vértessy, Kinga Nyíri   +6 more
wiley   +1 more source

Surface currents in the Mid-Atlantic Bight: the role of the Gulf Stream versus wind

Frontiers in Marine Science
Surface currents of the Mid-Atlantic Bight (MAB) were studied using high-frequency radar (HFR) observations at 6 km resolution during a 5-year period (2020–2024). The study’s focus on the role of the Gulf Stream (GS) contrasts with most past studies that
Tal Ezer
doaj   +1 more source

HIV‐1 establishes immediate latency in T cells expressing the viral Nef protein

FEBS Open Bio, EarlyView.
Nef is a viral protein often omitted from HIV‐1 reporter viruses. Consequently, its role in viral latency is unclear. We developed three novel dual reporter HIV‐1 derivatives that express Nef and allow for detection of latent and productive infection. Using these reporters, we show that Nef does not affect the establishment of immediate viral latency ...
Cindy Lam, Ivan Sadowski
wiley   +1 more source

Alcohol‐induced altered glycans in human tracheal epithelial cells promote bacterial adhesion

FEBS Open Bio, EarlyView.
Alcohol induces altered glycans to promote bacteria adhesion. Heavy alcohol drinking is known to increase the risk of bacterial pneumonia. However, the link between alcohol levels and risk of infection remains underexplored. Recently, we found that alcohol induced α2‐6sialo mucin O‐glycans in human tracheobronchial epithelial cells, which mediated the ...
Pi‐Wan Cheng, Souvik Datta, Derrick R. Samuelson   +2 more
wiley   +1 more source

The Impact of Air–Sea Flux Parameterization Methods on Simulating Storm Surges and Ocean Surface Currents

Journal of Marine Science and Engineering
As the primary driver of energy transfer between atmospheric and oceanic systems, the air–sea momentum flux fundamentally governs coupled model dynamics through its regulation of wind stress partitioning.
Li Cai, Bin Wang, Wenqian Wang, Xingru Feng   +3 more
doaj   +1 more source

Meta‐analysis fails to show any correlation between protein abundance and ubiquitination changes

FEBS Open Bio, EarlyView.
We analyzed over 50 published proteomics datasets to explore the relationship between protein levels and ubiquitination changes across multiple experimental conditions and biological systems. Although ubiquitination is often associated with protein degradation, our analysis shows that changes in ubiquitination do not globally correlate with changes in ...
Nerea Osinalde, Unai Alduntzin, Gorka Prieto, Ugo Mayor   +3 more
wiley   +1 more source

Assessing the Future ODYSEA Satellite Mission for the Estimation of Ocean Surface Currents, Wind Stress, Energy Fluxes, and the Mechanical Coupling Between the Ocean and the Atmosphere

Remote Sensing
Over the past decade, several studies based on coupled ocean–atmosphere simulations have shown that the oceanic surface current feedback to the atmosphere (CFB) leads to a slow-down of the mean oceanic circulation and, overall, to the so-called eddy ...
Marco Larrañaga, Lionel Renault, Alexander Wineteer, Marcela Contreras, Brian K. Arbic, Mark A. Bourassa, Ernesto Rodriguez   +6 more
doaj   +1 more source