Results 121 to 130 of about 5,049,215 (291)

Mercury Content of Waters in the Midcontinent Region [PDF]

open access: yes, 1980
Two major areas of the midcontinent region were investigated for their aqueous mercury concentrations. Sixteen surface water and 17 ground water samples were collected in an eleven county area of N.W. Arkansas, S.W. Missouri and N.E. Oklahoma (Ozark area)
Barber, Larry, II, Steele, Kenneth F.
core   +2 more sources

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

open access: yesFEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli   +8 more
wiley   +1 more source

Enlarging Difference Between Persistent Extreme Precipitation and Fixed‐Time Extremes in China

open access: yesEarth's Future
Extreme precipitation occurs persistently in nature and has caused a series of social, environmental, and ecological problems. However, most analyses of extreme precipitation are conducted at fixed‐time intervals, which may lead to misconceptions of the ...
Yihui Zhang   +3 more
doaj   +1 more source

Chameleon sequences reveal structural effects in proteins representing micelle‐like distribution of hydrophobicity

open access: yesFEBS Open Bio, EarlyView.
Amino acids sequence of two different proteins with the same sequence (chameleon sequence—black boxes) represent in 3D structure of the proteins different secondary structures: HHHH—helical and BBB—Beta‐structural. The chains folded in water environment adopt different III‐order structures in which the chameleon fragments appear to adopt similar status
Irena Roterman   +4 more
wiley   +1 more source

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

open access: yesFEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen   +9 more
wiley   +1 more source

Declining Contribution of Plant Physiological Effects to Global Drought Characteristics With Rising CO2 Using State‐of‐the‐Art Earth System Models

open access: yesEarth's Future
Vegetation physiology responses to rising atmospheric CO2 can alter the global hydrological cycle, thereby influencing drought occurrence. It has long been controversial and poorly understood how vegetation physiological effects influence meteorological ...
Ziwei Li   +3 more
doaj   +1 more source

Matrix metalloproteinase‐9 regulates cell adhesion and membrane protrusive activity of ovarian cancer cells

open access: yesFEBS Open Bio, EarlyView.
Matrix metalloproteinase‐9 (MMP9) drives ovarian cancer progression. Using MMP9‐null cells (M9‐KO) created from ovarian cancer cells, we found MMP9 loss did not block Epidermal Growth Factor (EGF)‐driven E‐cadherin dissolution or EMT but delayed and reduced EGF‐driven membrane protrusions. Transient MMP9 re‐expression drove membrane protrusion.
Claire Strauel   +8 more
wiley   +1 more source

Quantitative proteomic analysis reveals different characteristics of bladder cancer cells after exposure to bisphenol A

open access: yesFEBS Open Bio, EarlyView.
Bisphenol A (BPA), a common chemical in plastics, exerts dual effects on bladder cancer cells: low doses promote growth and migration, while high doses suppress growth and migration. Multi‐omics and bioinformatics reveal BPA acts via MAPK and inflammatory pathways.
Shaomin Niu   +10 more
wiley   +1 more source

Interpretable flash flood susceptibility mapping in Yarlung Tsangpo River Basin using H2O Auto-ML

open access: yesScientific Reports
Flash flood susceptibility mapping is essential for identifying areas prone to flooding events and aiding decision-makers in formulating effective prevention measures.
Fei He   +3 more
doaj   +1 more source

Rapid screening of staphylokinase protein variants using an unpurified cell‐free expression system

open access: yesFEBS Open Bio, EarlyView.
An unpurified cell‐free protein synthesis (CFPS) platform enables rapid functional screening of staphylokinase variants. Direct plasminogen‐activation assays performed in microplate format provide real‐time activity readouts, allowing rapid identification and ranking of variants with improved or reduced fibrinolytic activity without protein ...
Maria Tomková   +3 more
wiley   +1 more source

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