Results 141 to 150 of about 6,240,497 (308)

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

Evaluation of in-vitro antibiotic susceptibility of different morphological forms of Borrelia burgdorferi

Infection and Drug Resistance, 2011
Eva Sapi1, Navroop Kaur1, Samuel Anyanwu1, David F Luecke1, Akshita Datar1, Seema Patel1, Michael Rossi1, Raphael B Stricker21Lyme Disease Research Group, Department of Biology and Environmental Sciences, University of New Haven, New Haven, CT, USA ...
Sapi E, Kaur N, Anyanwu S, Luecke DF, Datar A, Patel S, Rossi M, Stricker RB   +7 more
doaj  

Protocol for quantifying miRNA trafficking across the endosomal membrane

FEBS Open Bio, EarlyView.
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh, Kamalika Mukherjee, Suvendra N. Bhattacharyya   +2 more
wiley   +1 more source

An approach for predicting landslide susceptibility and evaluating predisposing factors

International Journal of Applied Earth Observation and Geoinformation
Effectively leveraging landslide spatial location information is crucial for improving the accuracy of deep learning in predicting landslide susceptibility and exploring the impacts of predisposing factors. Current single deep learning models for landslide susceptibility assessment require enhancements in both prediction accuracy and robustness ...
Wanxin Guo, Jian Ye, Chengbing Liu, Yijie Lv, Qiuyu Zeng, Xin Huang   +5 more
openaire   +3 more sources

Susceptibility assessment of geological hazards in Shenzhen Town, Ninghai county based on the APH-CF model

Frontiers in Earth Science
IntroductionThis work employs a coupled evaluation model that integrates deterministic coefficients with the Analytic Hierarchy Process to conduct a comprehensive assessment of geological disaster susceptibility in Shenzhen Town, Ninghai County ...
Shuai Han, Ran Li, Ran Li, Ran Li, Shujun Hui, Qiang Sun, Taili Zhang   +6 more
doaj   +1 more source

Polymer Electrochromism Driven by Metabolic Activity Facilitates Rapid and Facile Bacterial Detection and Susceptibility Evaluation. [PDF]

Adv Funct Mater, 2020
Wu J, Zhu Y, You L, Dong PT, Mei J, Cheng JX.   +5 more
europepmc   +1 more source

An Independent Evaluation

, 2015
The year 2015 is pivotal in international development. As the era of the Millennium Development Goals (MDGs) concludes, work continues to take forward the unfinished MDG agenda and to define and meet the
Independent Evaluation Group
core  

Avidin is evolutionarily conserved in fish but dispensable for development and resistance against Streptococcus agalactiae in zebrafish

FEBS Open Bio, EarlyView.
The presence of biotin‐binding avidin proteins in fish and their biological significance are poorly characterized. We cataloged fish avidins and demonstrate that they are widely present and evolutionarily conserved. We created avd knockout zebrafish and show that zebavidin is dispensable for development and that resistance of avd knockout embryos in ...
Anni K. Saralahti, Markus J. T. Ojanen, Mataleena Parikka, Otto Kauko, Mika Rämet, Vesa P. Hytönen   +5 more
wiley   +1 more source

GelMA‐based 3D spheroids recapitulate transcriptomic and functional hallmarks of myeloid sarcoma

FEBS Open Bio, EarlyView.
GelMA 5% hydrogels support the formation of myeloid leukemia spheroids that recapitulate MS‐specific features, including G1 arrest, apoptosis, and ECM‐driven transcriptomic reprogramming. The 3D model mimicked soft‐tissue‐like stiffness and oxygen conditions, and transcriptomic convergence with primary MS samples confirmed its utility as a preclinical ...
Nicolas Germain, Elise Buret, Le Hoang Thanh Nguyen, Marion Cocheteux, Nicolas Touya, Mélanie Dhayer, Romain Dubois, Frank Lafont, Sebastien Janel, Patrice Maboudou, Steve Lancel, Philippe Marchetti   +11 more
wiley   +1 more source

Evaluation and the Environmental Democracy of European Cities

, 2010
Since its early days, environmental assessment aroused high expectations about its ability to insinuate ecological rationalities into worldviews, institutions, and social practices.
Alessandro Bonifazi
core