Replacement of Albumin by Preovulatory Oviductal Fluid in Swim-Up Sperm Preparation Method Modifies Boar Sperm Parameters and Improves In Vitro Penetration of Oocytes [PDF]
More suitable and efficient methods to protect gametes from external harmful effects during in vitro handling can be achieved by adding preovulatory porcine oviductal fluid (pOF) to in vitro culture media.
Sergio Navarro-Serna +4 more
doaj +2 more sources
Swim‐up method is superior to density gradient centrifugation for preserving sperm DNA integrity during sperm processing [PDF]
Purpose This study aimed to evaluate the effects of swim‐up and density gradient centrifugation methods on sperm DNA fragmentation. Methods Nineteen normozoospermic patient samples with ≥100 × 106 motile sperms were included in this study.
Kenji Amano +15 more
doaj +2 more sources
Density gradient centrifugation versus swim-up for selecting spermatozoa with improved redox activity, calcium status, and DNA integrity in an established rat model of varicocele-induced infertility [PDF]
BackgroundVaricocele (VCL) is a frequent pathological condition associated with impaired sperm quality, oxidative stress, altered calcium homeostasis, and sperm DNA fragmentation.
Ali Mutashar Naeemah Al-Hamdawee +3 more
doaj +2 more sources
Comparative Sensitivity of Canine Swim-Up Sperm Fractions Treated with Antifreeze Protein and Trehalose Following Freeze–Thaw [PDF]
Sperm cryopreservation is widely used in reproductive biotechnology, but the freeze–thaw process often reduces sperm quality by impairing motility, viability, and DNA integrity.
Abbas Farshad +2 more
doaj +2 more sources
Upstream or swim up processing technique: which one is more effective to select human sperm with high chromatin integrity [PDF]
Background: Sperm processing methods separate motile sperms with good morphology from dead and abnormal forms of sperms, immature germ cells, and non-sperm cells.
Mahnaz Heidari +9 more
doaj +2 more sources
Efficacy of Hyaluronic Acid in The Selection of Human Spermatozoa with Intact DNA by The Swim-up Method [PDF]
Objective: In 2014, enrolled 20 patients who applied to the Unit of Assisted Reproduction Techniques, Konya Necmettin Erbakan University. Based on the presence of hyaluronic acid (HA) in the oocyte-cumulus cell complex, sperm attached to HA in vivo ...
Aslihan Saylan, Selcuk Duman
doaj +2 more sources
Context: In the last 10 years, assisted reproductive technologies (ARTs) have offered infertile couples an opportunity to complete their reproductive project.
Salvatore Raimondo +7 more
doaj +1 more source
بررسی بهبود پارامترهای اسپرم انسان بعد از آماده سازی به روش Swim up [PDF]
مقدمه: حدود10 الی15 درصد زوجها دارای مشکل ناباروری اند و برای آنهایی که به درمان دارویی پاسخ نمی دهند استفاده ازروشهای کمک باروری، اجتناب ناپذیر است. swim up یکی از روشهایی است که باعث بهبود کیفیت اسپرم میشود.
زهرا بصیرت +1 more
doaj +1 more source
Methods for seminal plasma (SP) removal and the selection of collared peccary sperm for fertilization were compared. The experiments evaluated the following: the (I) impact of centrifugation for SP removal before swim-up for sperm selection and (II) a ...
Maria V. O. Santos +7 more
doaj +1 more source
Profound Transcriptomic Differences Found between Sperm Samples from Sperm Donors vs. Patients Undergoing Assisted Reproduction Techniques Tends to Disappear after Swim-up Sperm Preparation Technique [PDF]
Background Although spermatozoa delivers its RNA to oocytes at fertilization, its biological role is not well characterized. Our purpose was to identify the genes differentially and exclusively expressed in sperm samples both before and after the swim-up
Sandra García-Herrero +5 more
doaj +1 more source

