Action of swim-up and caffeine on equine frozen sperm. [PDF]
Anim Reprod, 2022 Cryopreservation of equine semen is crucial to semen commercialization. However, it reduces sperm motility and longevity. Thus, sperm selection methods and addition of motility-activating substances to sperm, such as caffeine, may improve sperm quality of equine frozen semen.
Alves NC +6 more
europepmc +4 more sources
Replacement of Albumin by Preovulatory Oviductal Fluid in Swim-Up Sperm Preparation Method Modifies Boar Sperm Parameters and Improves In Vitro Penetration of Oocytes [PDF]
Animals, 2021 More suitable and efficient methods to protect gametes from external harmful effects during in vitro handling can be achieved by adding preovulatory porcine oviductal fluid (pOF) to in vitro culture media.
Sergio Navarro-Serna +4 more
doaj +2 more sources
Sperm swim-up techniques and DNA fragmentation [PDF]
Human Reproduction, 2001 Swim-up techniques for sperm separation may have detrimental effects on sperm DNA. We wished to determine whether the normal swim-up method with centrifugation used in our laboratory, which involves a centrifugation step, was harmful to sperm compared with swim-up without centrifugation.Semen samples were obtained from patients undergoing IVF or ...
Robert F Casper
exaly +3 more sources
Swim‐up method is superior to density gradient centrifugation for preserving sperm DNA integrity during sperm processing [PDF]
Reproductive Medicine and BiologyPurpose This study aimed to evaluate the effects of swim‐up and density gradient centrifugation methods on sperm DNA fragmentation. Methods Nineteen normozoospermic patient samples with ≥100 × 106 motile sperms were included in this study.
Kenji Amano +15 more
doaj +2 more sources
Effect of zinc on sperm recovered by swim-up. [PDF]
J Assist Reprod GenetZinc is known to influence chromatin stability, motility and protection against oxidative stress. While swim-up remains the preferred method for selecting sperm in Assisted Reproductive Technologies (ART), concerns arise regarding sperm DNA fragmentation associated with this procedure. Given zinc's significant role in protecting sperm DNA integrity and
Faggi M +3 more
europepmc +3 more sources
Comparison of the effect of different media on the clinical outcomes of the density-gradient centrifugation/swim-up and swim-up methods. [PDF]
Clin Exp Reprod Med, 2015 Sperm must be properly prepared in in vitro fertilization (IVF)-embryo transfer (ET) programs in order to control the fertilization rate and ensure that embryos are of high quality and have appropriate developmental abilities. The objective of this study was to determine the most optimal sperm preparation method for IVF.Patients less than 40 years of ...
Kim EK +5 more
europepmc +4 more sources
A Comparison of Swim-up and Simple Swim-up Methods for the Extraction of High Motility Sperm
Siriraj Medical Journal, 2020 The objective of this study was to compare two methods for the extraction of high motility sperm; swim-up and simple swim-up technique. The results were comparable in concentration, motility and morphology.
Singpetch Suksompong +4 more
doaj +1 more source
Comparative proteomic analysis of spermatozoa isolated by swim-up or density gradient centrifugation. [PDF]
Reprod Biol Endocrinol, 2015 BACKGROUND: Reports about the morphologic and functional characteristics of spermatozoa prepared by density gradient centrifugation (DC) or swim-up (SU) have produced discordant results.
Luppi S +6 more
europepmc +4 more sources
Upstream or swim up processing technique: which one is more effective to select human sperm with high chromatin integrity [PDF]
International Journal of Reproductive BioMedicine, 2018 Background: Sperm processing methods separate motile sperms with good morphology from dead and abnormal forms of sperms, immature germ cells, and non-sperm cells.
Mahnaz Heidari +9 more
doaj +2 more sources
Efficacy of Hyaluronic Acid in The Selection of Human Spermatozoa with Intact DNA by The Swim-up Method [PDF]
Cell Journal, 2016 Objective: In 2014, enrolled 20 patients who applied to the Unit of Assisted Reproduction Techniques, Konya Necmettin Erbakan University. Based on the presence of hyaluronic acid (HA) in the oocyte-cumulus cell complex, sperm attached to HA in vivo ...
Aslihan Saylan, Selcuk Duman
doaj +2 more sources