Results 1 to 10 of about 356,493 (148)

Swim‐up method is superior to density gradient centrifugation for preserving sperm DNA integrity during sperm processing [PDF]

Reproductive Medicine and Biology
Purpose This study aimed to evaluate the effects of swim‐up and density gradient centrifugation methods on sperm DNA fragmentation. Methods Nineteen normozoospermic patient samples with ≥100 × 106 motile sperms were included in this study.
Kenji Amano, Satoko Oigawa, Koichiro Ichizawa, Yukiko Tokuda, Mami Unagami, Mami Sekiguchi, Mayuko Furui, Kentaro Nakaoka, Ayumu Ito, Rika Hayashi, Yuko Tamaki, Yuko Hayashi, Yusuke Fukuda, Yukiko Katagiri, Masahiko Nakata, Koichi Nagao   +15 more
doaj   +2 more sources

Replacement of Albumin by Preovulatory Oviductal Fluid in Swim-Up Sperm Preparation Method Modifies Boar Sperm Parameters and Improves In Vitro Penetration of Oocytes [PDF]

Animals, 2021
More suitable and efficient methods to protect gametes from external harmful effects during in vitro handling can be achieved by adding preovulatory porcine oviductal fluid (pOF) to in vitro culture media.
Sergio Navarro-Serna, Evelyne París-Oller, Ondrej Simonik, Raquel Romar, Joaquín Gadea   +4 more
doaj   +2 more sources

Upstream or swim up processing technique: which one is more effective to select human sperm with high chromatin integrity [PDF]

International Journal of Reproductive BioMedicine, 2018
Background: Sperm processing methods separate motile sperms with good morphology from dead and abnormal forms of sperms, immature germ cells, and non-sperm cells.
Mahnaz Heidari, Niknam Lakpour, Niknam Lakpour, Mahsa Darbandi, Sarai Darban, Saeideh Shani, Leila Goharbakhsh, Ghazaleh Cheshmi, Mohammad Mehdi Akhondi, Mohammad Reza Sadeghi   +9 more
doaj   +2 more sources

Efficacy of Hyaluronic Acid in The Selection of Human Spermatozoa with Intact DNA by The Swim-up Method [PDF]

Cell Journal, 2016
Objective: In 2014, enrolled 20 patients who applied to the Unit of Assisted Reproduction Techniques, Konya Necmettin Erbakan University. Based on the presence of hyaluronic acid (HA) in the oocyte-cumulus cell complex, sperm attached to HA in vivo ...
Aslihan Saylan, Selcuk Duman
doaj   +2 more sources

Novel sperm selection device on the basis of microfluidics improves usable blastocyst rates, embryo morphology, and morphokinetic patterns: sibling cohort prospective study [PDF]

F&S Reports
Objective: To assess the performance of a novel microfluidic sperm selection device, SwimCount Harvester (SCH), compared with the Swim-up (SU) method, analyzing both sperm quality and the laboratory’s key performance indicators.
Fernando Meseguer, M.Sc., Laura Carrión-Sisternas, M.Sc., Ana del Arco, M.Sc., Rocío Rivera-Egea, Ph.D., Carmen Vidal, Ph.D., José Alejandro Remohí, Ph.D., Marcos Meseguer, Ph.D.   +6 more
doaj   +2 more sources

Sperm preparation techniques affect biological parameters, fertility indices, and DNA fragmentation in patients with oligo-astheno-teratozoospermia [PDF]

Reproduction and Fertility
In normozoospermia, the albumin gradient filtration (AGF) and swim-up processing techniques are used to isolate spermatozoa with the highest biological parameters and specific sperm fertility indices (SSFI).
Banafsheh Heidari, Amin Nosrati, Mostafa Pournourali, Farhad Seif, Nazanin Akbari, Abolfazl Shirazi   +5 more
doaj   +2 more sources

EFFECT OF THE USE OF TWO SPERM SELECTION TECHNIQUES FOR IN VITRO PRODUCTION OF ALPACA EMBRYOS [PDF]

SPERMOVA, 2021
The objective of this research was to evaluate the effect of the use of two sperm selection techniques for in vitro production of alpaca embryos. The ovaries and testis were collected from the local slaughterhouse and transport to 37 ° C in saline ...
Mijaíl Contreras Huamani, Mary Luz Naveros, Cesar A. Olaguivel   +2 more
doaj   +1 more source

Comparing different sperm separation techniques for ART, through quantitative evaluation of p53 protein

Journal of Human Reproductive Sciences, 2020
Context: In the last 10 years, assisted reproductive technologies (ARTs) have offered infertile couples an opportunity to complete their reproductive project.
Salvatore Raimondo, Tommaso Gentile, Mariacira Gentile, Francesca Donnarumma, Giusy Esposito, Alessia Morelli, Stefania De Filippo, Felice Cuomo   +7 more
doaj   +1 more source

بررسی بهبود پارامترهای اسپرم انسان بعد از آماده سازی به روش Swim up [PDF]

Majallah-i Zanān, Māmā̓ī va Nāzā̓ī-i Īrān, 2008
مقدمه: حدود10 الی15 درصد زوجها دارای مشکل ناباروری اند و برای آنهایی که به درمان دارویی پاسخ نمی دهند استفاده ازروشهای کمک باروری، اجتناب ناپذیر است. swim up یکی از روشهایی است که باعث بهبود کیفیت اسپرم میشود.
زهرا بصیرت, سید غلامعلی جورسرایی   +1 more
doaj   +1 more source

Profound Transcriptomic Differences Found between Sperm Samples from Sperm Donors vs. Patients Undergoing Assisted Reproduction Techniques Tends to Disappear after Swim-up Sperm Preparation Technique [PDF]

International Journal of Fertility and Sterility, 2010
Background Although spermatozoa delivers its RNA to oocytes at fertilization, its biological role is not well characterized. Our purpose was to identify the genes differentially and exclusively expressed in sperm samples both before and after the swim-up
Sandra García-Herrero, Nicolás Garrido, José Antonio Martínez-Conejero, José Remohí, Antonio Pellicer, Marcos Meseguer   +5 more
doaj   +1 more source