Results 31 to 40 of about 20,693 (273)

Automated FRAP microscopy for high‐throughput analysis of protein dynamics in chromatin organization and transcription

FEBS Open Bio, EarlyView.
RoboMic is an automated confocal microscopy pipeline for high‐throughput functional imaging in living cells. Demonstrated with fluorescence recovery after photobleaching (FRAP), it integrates AI‐driven nuclear segmentation, ROI selection, bleaching, and analysis.
Selçuk Yavuz, Bart Geverts, Johan A. Slotman, Andrea Sacchetti, Stefan Prekovic, Martin E. van Royen, Adriaan B. Houtsmuller   +6 more
wiley   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

A Depolarizing Leak in Sodium Bicarbonate Cotransporter NBCe1 Causes Brain Edema

Annals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Objectives SLC4A4 encodes electrogenic sodium bicarbonate cotransporter NBCe1, prominently expressed in kidney and brain. Recessive loss‐of‐function variants in SLC4A4 cause proximal renal tubular acidosis, no brain edema. In the brain, NBCe1 is expressed by astrocytes, where it regulates pH and mediates astrocyte volume changes.
Quinty Bisseling, Mark D. Parker, Sven Kerst, Richard A. Pasternack, Jacob Tondreau, Marjolein Breur, Gemma M. van Rooijen‐van Leeuwen, Davide Tonduti, Ettore Salsano, Alejandra Darling, Joanna A. E. van Wijk, Susanna Törnroth‐Horsefield, Marianna Bugiani, Petra J. W. Pouwels, Quinten Waisfisz, Marjo S. van der Knaap, Rogier Min   +16 more
wiley   +1 more source

Triple-Voltage Gain and Self-Balancing in a New Switched-Capacitor Seven-Level Inverter for Microgrid Integration

Energies
In the context of power electronic interfaces in photovoltaic (PV), fuel cell, battery, and microgrid applications, the low output voltage of the DC source necessitates a voltage-boosting inverter. This paper proposes a single-source seven-level switched-
Mohamed Salem, Mahmood Swadi, Anna Richelli, Yevgeniy Muralev, Faisal A. Mohamed   +4 more
doaj   +1 more source

Integrated chaos generators [PDF]

, 2002
This paper surveys the different design issues, from mathematical model to silicon, involved on the design of integrated circuits for the generation of chaotic behavior.Comisión Interministerial de Ciencia y Tecnología 1FD97-1611(TIC)European Commission ...
Delgado Restituto, Manuel, Rodríguez Vázquez, Ángel Benito   +1 more
core   +1 more source

A Robust Adaptive One‐Sample‐Ahead Preview Super‐Twisting Sliding Mode Controller

International Journal of Adaptive Control and Signal Processing, EarlyView.
Block Diagram of the Robust Adaptive One‐Sample‐Ahead Preview Super‐Twisting Sliding Mode Controller. ABSTRACT This article introduces a discrete‐time robust adaptive one‐sample‐ahead preview super‐twisting sliding mode controller. A stability analysis of the controller by Lyapunov criteria is developed to demonstrate its robustness in handling both ...
Guilherme Vieira Hollweg, Paulo Jefferson Dias de Oliveira Evald, Shivam Chaturvedi, Hilton Abilio Gründling, Rodrigo Varella Tambara, Wencong Su   +5 more
wiley   +1 more source

An Improved and Efficient High-Boost Switched Capacitor Multilevel Inverter

Power Electronics and Drives
This paper presents a fundamental nine-level switched-capacitor multilevel inverter (CSMLI) powered by a single DC source. The configuration employs two capacitors, nine switches, and one diode to generate nine distinct output voltage levels. It achieves
Kar Pratik, Bagarty Durgesh Prasad, Ray Prakash Kumar, Sarangi Rachita Ruchismita, Mohanty Asit   +4 more
doaj   +1 more source

Planar Solid‐State Nanopores Toward Scalable Nanofluidic Integration Based on CMOS Technology

Advanced Engineering Materials, EarlyView.
We present a scalable silicon‐based fabrication strategy for planar solid‐state nanopores to enable their integration with complex nanofluidic systems. Prototype devices demonstrate normal voltage‐current characteristics, good noise performance, and appreciable streaming currents. Our CMOS‐compatible fabrication process offers precise geometric control
Ngan Hoang Pham, Chenyu Wen, Mohammad Hadi Khaksaran, Won‐Yong Lee, Javier Cruz, Klas Hjort, Dongping Wu, Shi‐Li Zhang   +7 more
wiley   +1 more source

A New 19-Level Switched Capacitor Multilevel Inverter With Self-Managing Voltage Balance

IEEE Access
This paper introduces a novel single-phase multilevel inverter utilizing a switched-capacitor technique that generates 19-levels while minimizing component count to 12 switches, three diodes, two DC sources, and three capacitors.
Seyed Hadi Shafiei, Yousef Seifi Kavian, Karim Ansari-Asl   +2 more
doaj   +1 more source

Analog integrated neural-like circuits for nonlinear programming [PDF]

, 1989
A systematic approach for the design of analog neural nonlinear programming solvers using switched-capacitor (SC) integrated circuit techniques is presented. The method is based on formulating a dynamic gradient system whose state evolves in time towards
Domínguez Castro, Rafael, Huertas Díaz, José Luis, Rodríguez Vázquez, Ángel Benito, Sánchez Sinencio, Edgar   +3 more
core   +1 more source