Results 31 to 40 of about 75,692 (266)

Methodological insights into the dip-and-pull X-ray photoelectron spectroscopy technique: analysing electrochemical interfaces under in situ/operando conditions

Journal of Synchrotron Radiation
Gaining insight into structural and compositional transformations occurring at the electrode/electrolyte interface during the operation of electrochemical systems is fundamental to understanding and, thus, optimizing their performance.
Benjamin Rotonnelli, Amandine Brige, Alexandr G. Oshchepkov, Jean-Jacques Gallet, Fabrice Bournel, Antoine Bonnefont, Alexander Yaroslavtsev, Andrey Shavorskiy, Robert Temperton, Elena R. Savinova, Tristan Asset   +10 more
doaj   +1 more source

High-pressure single-crystal diffraction at the Australian Synchrotron

Journal of Synchrotron Radiation, 2023
A new high-pressure single-crystal diffraction setup has been designed and implemented at the Australian Synchrotron for collecting molecular and protein crystal structures. The setup incorporates a modified micro-Merrill–Bassett cell and holder designed
Stephanie A. Boer, Jason R. Price, Alan Riboldi-Tunnicliffe, Rachel Williamson, Robert Rostan, Aston Summers, Gemma F. Turner, Isabelle Jones, Charles S. Bond, Alice Vrielink, Andrew C. Marshall, John Hitchings, Stephen A. Moggach   +12 more
doaj   +1 more source

Synchrotron-Radiation-Based Fourier Transform Infrared Microspectroscopy as a Tool for the Differentiation between Staphylococcal Small Colony Variants

Antibiotics, 2022
Small colony variants (SCVs) are clinically significant and linked to persistent infections. In this study, synchrotron-radiation-based Fourier transform infrared (SR-FTIR) is used to investigate the microspectroscopic differences between the SCVs of ...
Amal G. Al-Bakri, Lina A. Dahabiyeh, Enam Khalil, Deema Jaber, Gihan Kamel, Nina Schleimer, Christian Kohler, Karsten Becker   +7 more
doaj   +1 more source

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

FEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei, Fredrik Orädd, Konstantinos Magkakis, Magnus Andersson   +3 more
wiley   +1 more source

Combined Genomic and Imaging Techniques Show Intense Arsenic Enrichment Caused by Detoxification in a Microbial Mat of the Dead Sea Shore

Geochemistry, Geophysics, Geosystems
Microbial mats and microbialites are essential tools for reconstructing early life and its environments. To better understand microbial trace element cycling, a microbial mat was collected from the sinkhole systems of the western shores of the Dead Sea ...
C. Thomas, M. Filella, D. Ionescu, S. Sorieul, C. G. L. Pollier, A. M. Oehlert, P. Zahajská, N. Gedulter, A. Agnon, D. Ferreira Sanchez, D. Ariztegui   +10 more
doaj   +1 more source

3-Hydroxy-2-[(4-hydroxybenzyl)azaniumyl]propanoate monohydrate

IUCrData, 2017
In the title hydrated zwitterion, C10H13NO4·H2O, the N—C—C—OH side chain shows a gauche conformation [torsion angle = −59.05 (18)°]. In the crystal, the components are linked by O—H...O, N—H...O and C—H...O hydrogen bonds to generate a three-dimensional ...
Min Gao, Li-Ping Lu
doaj   +1 more source

Investigating the Impact of Synchrotron THz Radiation on the Corneal Hydration Using Synchrotron THz-Far Infrared Beamline

Sensors, 2022
Due to increasing interest in imaging, industrial, and the development of wireless communication operating at THz frequencies, it is crucial to ascertain possible health impacts arising from exposure to THz radiation.
Negin Foroughimehr, Zoltan Vilagosh, Ali Yavari, Andrew Wood   +3 more
doaj   +1 more source

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

FEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill, Yelyzaveta Pulnova, Elke De Zitter, Helen M. Ginn, Briony A. Yorke   +4 more
wiley   +1 more source

Evolutionarily divergent DUF4465 domains have a common vitamin B12‐binding function

FEBS Open Bio, EarlyView.
We show that DUF4465 family proteins, widespread across bacteria from gut microbiomes, hydrothermal vents, and soil, share a common vitamin B12‐binding function. These augmented β‐jellyroll proteins bind vitamin B12 via extended loops. Our findings establish sequence‐diverse DUF4465 proteins as a widespread class of B12‐binding proteins, highlighting ...
Charlea Clarke, Michal Banasik, Rokas Juodeikis, Martin J. Warren, Richard W. Pickersgill   +4 more
wiley   +1 more source

Versatile vector tools for efficient protein screening across multiple expression systems

FEBS Open Bio, EarlyView.
A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu, Yaqing Liu, Lei Qin, Feng Yu, Qisheng Wang, Yahui Liu   +5 more
wiley   +1 more source