Results 51 to 60 of about 357,376 (300)

Structural insights into an engineered feruloyl esterase with improved MHET degrading properties

FEBS Letters, EarlyView.
A feruloyl esterase was engineered to mimic key features of MHETase, enhancing the degradation of PET oligomers. Structural and computational analysis reveal how a point mutation stabilizes the active site and reshapes the binding cleft, expading substrate scope.
Panagiota Karampa, Konstantinos Makryniotis, Theofani‐Iosifina Sousani, Evangelos Topakas, Vangelis Daskalakis, Maria Dimarogona   +5 more
wiley   +1 more source

A methionine‐lined active site governs carbocation stabilization and product specificity in a bacterial terpene synthase

FEBS Letters, EarlyView.
This study reveals a unique active site enriched in methionine residues and demonstrates that these residues play a critical role by stabilizing carbocation intermediates through novel sulfur–cation interactions. Structure‐guided mutagenesis further revealed variants with significantly altered product profiles, enhancing pseudopterosin formation. These
Marion Ringel, Carl P. O. Helmer, Shani Zev, Ronja Driller, Emily Buhr, Markus Reinbold, Renana Schwartz, Gabriel Foley, Mikael Boden, Daniel Garbe, Gerhard Schenk, Dan Thomas Major, Bernhard Loll, Thomas Brück   +13 more
wiley   +1 more source

Simulations of particle acceleration beyond the classical synchrotron burnoff limit in magnetic reconnection: An explanation of the Crab flares [PDF]

, 2013
It is generally accepted that astrophysical sources cannot emit synchrotron radiation above 160 MeV in their rest frame. This limit is given by the balance between the accelerating electric force and the radiation reaction force acting on the electrons ...
Begelman, Mitchell C., Cerutti, Benoit, Uzdensky, Dmitri A., Werner, Gregory R.   +3 more
core   +1 more source

Comparison of the 3D-microstructure of human alveolar and fibula bone in microvascular autologous bone transplantation: a synchrotron radiation μ-CT study

Frontiers in Bioengineering and Biotechnology, 2023
Introduction: Autologous bone transplantation is successfully used in reconstructive surgery of large/critical-sized bone defects, whereby the microvascular free fibula flap is still regarded as the gold standard for the reconstruction of such defects in
Jonas Wüster, Bernhard Hesse, Bernhard Hesse, Rene Rothweiler, Emely Bortel, Christian Gross, Shima Bakhtiyari, Andrew King, Elodie Boller, Javier Gerber, Carsten Rendenbach, Tobias Fretwurst, Saskia Preissner, Max Heiland, Katja Nelson, Susanne Nahles   +15 more
doaj   +1 more source

Synchrotron Self-Compton Model for Rapid Nonthermal Flares in Blazars with Frequency-Dependent Time Lags [PDF]

, 2004
We model rapid variability of multifrequency emission from blazars occurring across the electromagnetic spectrum (from radio to gamma-rays). Lower energy emission is produced by the synchrotron mechanism, whereas higher energy emission is due to inverse ...
Alan P. Marscher, Andrei Sokolov, Coppi P. S., Dubal M. R., Ghisellini G., Ian M. McHardy, Komissarov S. S., Marti J. M., Mastichiadis A., Mastichiadis A.   +9 more
core   +2 more sources

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

FEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei, Fredrik Orädd, Konstantinos Magkakis, Magnus Andersson   +3 more
wiley   +1 more source

Independent Evidence for the Preservation of Endogenous Bone Biochemistry in a Specimen of Tyrannosaurus rex

Biology, 2023
Biomolecules preserved in deep time have potential to shed light on major evolutionary questions, driving the search for new and more rigorous methods to detect them.
Jennifer Anné, Aurore Canoville, Nicholas P. Edwards, Mary H. Schweitzer, Lindsay E. Zanno   +4 more
doaj   +1 more source

Blazar sequence - an artefact of Doppler boosting

, 2008
The blazar sequence is a scenario in which the bolometric luminosity of the blazar governs the appearance of its spectral energy distribution. The most prominent result is the significant negative correlation between the synchrotron peak frequencies and ...
Antón, Blandford, Caccianiga, Donato, E. Nieppola, E. Valtaoja, Fossati, Ghisellini, Ghisellini, Guerra, Güijosa, Jorstad, Lähteenmäki, M. Kotiranta, M. Tornikoski, Nieppola, Nieppola, Padovani, Padovani, Padovani, Padovani, Padovani, Perlman, Pian, Readhead, Salonen, T. Hovatta, Takahashi, Teräsranta, Teräsranta, Teräsranta, Teräsranta, Verkhodanov, Wall, Wu   +34 more
core   +1 more source

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

FEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill, Yelyzaveta Pulnova, Elke De Zitter, Helen M. Ginn, Briony A. Yorke   +4 more
wiley   +1 more source

Large Language Model-Informed X-ray Photoelectron Spectroscopy Data Analysis

Signals
X-ray photoelectron spectroscopy (XPS) remains a fundamental technique in materials science, offering invaluable insights into the chemical states and electronic structure of a material.
J. de Curtò, I. de Zarzà, Gemma Roig, Carlos T. Calafate   +3 more
doaj   +1 more source