Results 91 to 100 of about 934,952 (299)

Synthetic biology: Understanding biological design from synthetic circuits [PDF]

, 2009
An important aim of synthetic biology is to uncover the design principles of natural biological systems through the rational design of gene and protein circuits.
A Becskei, A Becskei, A Becskei, A Friedland, A Kinkhabwala, A Levskaya, A Raj, A Raj, A Remenyi, A Wagner, AE Tsong, Alexander van Oudenaarden, B Kornmann, B Suess, BJ Yeh, BN Armbruster, BP Prud'homme, C Dugave, C Grilly, C Khosla, CC Guet, CH Martin, CJ Bashor, CL Beisel, D Grate, D Ro, D Rothlisberger, DD Young, DE Nelson, E Fung, E Ozbudak, E Segal, EA Davidson, ED Conrad, EH Davidson, EJ Steen, EM Ozbudak, ES Gilbert, F Jacob, FG Cruz, FJ Isaacs, FJ Isaacs, FK Balagadde, G Werstruck, I Cantone, I Golding, IA Swinburne, J Gerhart, J Gertz, J Gore, J Hasty, J Janin, J Kaplan, J Pedraza, J Stricker, JA Ubersax, JC Anderson, JE Dueber, JM Skerker, JR Pomerening, JR Pomerening, JS Chuang, JS Mattick, JT Bridgham, JT Mettetal, K Brenner, K Hammer, K Harris, K Tatebayashi, L Cai, L You, M Acar, M Behar, M Isalan, M Isalan, M Proft, M Rajendran, M Rapp, M Tigges, M-T Chen, MB Elowitz, MB Elowitz, MN McClean, MN Win, MR Atkinson, MS Antunes, N Geva-Zatorsky, N Rosenfeld, N Rosenfeld, NE Buchler, O Kwon, P Cironi, P Hersen, PL Howard, RJ Taylor, RP Bhattacharyya, RS Cox, S Basu, S Basu, S Hooshangi, S Pokharel, S Shimizu-Sato, SB Cambridge, Shankar Mukherji, SK Desai, T Bulter, T Ellis, TL Deans, TY Tsai, U Alon, Y Setty, Y Tanouchi, YT Maeda, Z Waks   +113 more
core   +1 more source

A methionine‐lined active site governs carbocation stabilization and product specificity in a bacterial terpene synthase

FEBS Letters, EarlyView.
This study reveals a unique active site enriched in methionine residues and demonstrates that these residues play a critical role by stabilizing carbocation intermediates through novel sulfur–cation interactions. Structure‐guided mutagenesis further revealed variants with significantly altered product profiles, enhancing pseudopterosin formation. These
Marion Ringel, Carl P. O. Helmer, Shani Zev, Ronja Driller, Emily Buhr, Markus Reinbold, Renana Schwartz, Gabriel Foley, Mikael Boden, Daniel Garbe, Gerhard Schenk, Dan Thomas Major, Bernhard Loll, Thomas Brück   +13 more
wiley   +1 more source

A synthetic biology approach for a vaccine platform against known and newly emerging serotypes of bluetongue virus [PDF]

, 2014
Bluetongue is one of the major infectious diseases of ruminants and is caused by Bluetongue virus (BTV), an arbovirus existing in nature in at least 26 distinct serotypes. Here, we describe the development of a vaccine platform for BTV.
Brunet, Silvie, Hamers, Claude, Hudelet, Pascal, Nunes, Sandro Filipe, Palmarini, Massimo, Ratinier, Maxime, Shaw, Andrew   +6 more
core   +2 more sources

Hyperosmotic stress induces PARP1‐mediated HPF1‐dependent mono(ADP‐ribosyl)ation

FEBS Letters, EarlyView.
Sorbitol‐induced hyperosmotic stress rapidly induces reversible mono(ADP‐ribosyl)ation (MARylation) on PARP1 without the signs of genotoxic signaling. We show that PARP1 autoMARylation is HPF1 dependent and forms hydroxylamine‐resistant O‐glycosidic linkages.
Anna Georgina Kopasz, Mihály Mérey, Rebeka Vásárhelyi, Ramóna Pék, Victor Imburchia, László Henn, Adrián Kószó, Nicholas D. Lakin, Ivan Ahel, Sébastien Huet, Ágnes Czibula, Gyula Timinszky   +11 more
wiley   +1 more source

Exosome-Mediated Chemoresistance in Cancers: Mechanisms, Therapeutic Implications, and Future Directions

Biomolecules
Chemotherapy resistance represents a formidable obstacle in oncological therapeutics, substantially compromising the efficacy of adjuvant chemotherapy regimens and contributing to unfavorable clinical prognoses.
Gengqi Liu, Jingang Liu, Silu Li, Yumiao Zhang, Ren He   +4 more
doaj   +1 more source

Linking neurogenesis, oligodendrogenesis, and myelination defects to neurodevelopmental disruption in primary mitochondrial disorders

FEBS Letters, EarlyView.
Mitochondrial remodeling shapes neural and glial lineage progression by matching metabolic supply with demand. Elevated OXPHOS supports differentiation and myelin formation, while myelin compaction lowers mitochondrial dependence, revealing mitochondria as key drivers of developmental energy adaptation.
Sahitya Ranjan Biswas, Porter L. Tomsick, Alicia M. Pickrell, Paul D. Morton   +3 more
wiley   +1 more source

Inducible chromosomal rearrangement reveals nonlinear polygenic dosage effects in driving aneuploid yeast traits

Nature Communications
Aneuploidy induces chromosomal scale alterations in gene dosage, impacting organismal proliferation yet serving as a driver for adaptive evolution.
Hongyi Yin, Zhou Guo, Chu Zhou, Lu Ma, Yi Wu, Ying-jin Yuan   +5 more
doaj   +1 more source

An isoform of 14‐3‐3 protein regulates transbilayer lipid movement at the plasma membrane

FEBS Letters, EarlyView.
Loss of 14‐3‐3ζ in CHO cells confers resistance to exogenous phosphatidylserine (PS) and impairs endocytosis‐independent inward flip‐flop of fluorescent PS at the plasma membrane. RNAi‐mediated knockdown reproduces this defect, while no additive effect is seen in ATP11C‐deficient cells.
Akiko Yamaji‐Hasegawa, Akira Hattori, Masafumi Tsujimoto, Toshihide Kobayashi   +3 more
wiley   +1 more source

Organizing the interface—Plasma membrane architecture and receptor dynamics in virus‐cell interactions

FEBS Letters, EarlyView.
Plasma membranes contain dynamic nanoscale domains that organize lipids and receptors. Because viruses operate at similar scales, this architecture shapes early infection steps, including attachment, receptor engagement, and entry. Using influenza A virus and HIV‐1 as examples, we highlight how receptor nanoclusters, multivalent glycan interactions ...
Jan Schlegel, Christian Sieben
wiley   +1 more source

Encryption and steganography of synthetic gene circuits

Nature Communications, 2018
Artificial gene circuits represent intellectual property that under some circumstances may need to be obfuscated to prevent discovery by third parties. Here the authors use encryption by overlapping recombinase sites and steganography by the introduction
Oliver Purcell, Jerry Wang, Piro Siuti, Timothy K. Lu   +3 more
doaj   +1 more source