Results 51 to 60 of about 13,766,532 (237)
Journal of Biological Engineering, 2023 Background Efficient cell-free protein expression from linear DNA templates has remained a challenge primarily due to template degradation. In addition, the yields of transcription in cell-free systems lag behind transcriptional efficiency of live cells.
Christopher Deich +9 more
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Minimal Out-of-Equilibrium Metabolism for Synthetic Cells: A Membrane Perspective
ACS Synthetic Biology, 2023 Life-like systems need to maintain a basal metabolism, which includes importing a variety of building blocks required for macromolecule synthesis, exporting dead-end products, and recycling cofactors and metabolic intermediates, while maintaining steady ...
Eleonora Bailoni +5 more
semanticscholar +1 more source
Synthetic Cells: From Simple Bio‐Inspired Modules to Sophisticated Integrated Systems
Angewandte Chemie, 2021 Bottom‐up synthetic biology is the science of building systems that mimic the structure and function of living cells from scratch. To do this, researchers combine tools from chemistry, materials science, and biochemistry to develop functional and ...
C. Guindani +4 more
semanticscholar +1 more source
DNA Origami Signaling Units Transduce Chemical and Mechanical Signals in Synthetic Cells
Advanced Functional Materials, 2023 Transmembrane proteins transmit chemical signals as well as mechanical cues. The latter is often achieved by coupling to the cytoskeleton. The incorporation of fully engineerable membrane‐spanning structures for the transduction of chemical and, in ...
Kevin Jahnke +5 more
semanticscholar +1 more source
Bottom-Up Assembly of Synthetic Cells with a DNA Cytoskeleton
ACS Nano, 2022 Cytoskeletal elements, like actin and myosin, have been reconstituted inside lipid vesicles toward the vision to reconstruct cells from the bottom up. Here, we realize the de novo assembly of entirely artificial DNA-based cytoskeletons with programmed ...
Kevin Jahnke +4 more
semanticscholar +1 more source
GeoV: An Open‐Source Software Package for Quantitative Image Analysis of 3D Vesicle Morphologies
Advanced Intelligent Systems, 2023 Bottom‐up synthetic biology has reconstituted processes like adhesion, cortex formation, or division of giant lipid vesicles (GUVs), which all rely on changes in the vesicle morphology.
Yannik Dreher +3 more
doaj +1 more source
STAR Protocols, 2023 Summary: Here, we present a protocol for controllable biomimetic mineralization at the nano-scale, simulating natural ion-enriched sedimentary mineralization. We describe steps for treatment of metal-organic frameworks with polyphenol-mediated stabilized
Xiaolong Zhan +8 more
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Proceedings of the National Academy of Sciences of the United States of America, 2022 Significance Synthetic cells, artificial cell-like particles, were engineered using synthetic biology tools to produce therapeutic proteins inside the body. Synthetic biology promises to overcome malfunctioning natural processes and treat disease.
Gal Chen +11 more
semanticscholar +1 more source
Division in synthetic cells [PDF]
Emerging Topics in Life Sciences, 2019 Cell division is one of the most fundamental processes of life, and so far the only known way of how living systems can come into existence at all. Consequently, its reconstitution in any artificial cell system that will have to be built from the bottom-up is a notoriously complex but an important task.
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Synthetic biology of minimal living cells: primitive cell models and semi-synthetic cells [PDF]
Systems and Synthetic Biology, 2010 This article summarizes a contribution presented at the ESF 2009 Synthetic Biology focused on the concept of the minimal requirement for life and on the issue of constructive (synthetic) approaches in biological research. The attempts to define minimal life within the framework of autopoietic theory are firstly described, and a short report on the ...
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