Results 151 to 160 of about 3,003,336 (196)
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Synthetic vehicles for DNA vaccination
Journal of Drug Targeting, 2009DNA vaccination is an attractive immunization method able to induce robust cellular immune responses in pre-clinical models. However, clinical DNA vaccination trials performed thus far have resulted in marginal responses. Consequently, strategies are currently under development to improve the efficacy of DNA vaccines. A promising strategy is the use of
van den Berg, J.H. +6 more
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The assessment of DNA-synthetic activity
Histochemistry, 1981A method is described by which a numerical value can be assigned to the amount of DNA-synthesis shown graphically by population-histograms obtained by Feulgen cytophotometry. The index appeared to give a reasonable measure of DNA-synthetic activity in populations having very low, moderate and high mitotic indices and closely followed labelling indices ...
L A, Coulton, B, Henderson, J, Chayen
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Synthetic DNA delivery systems
Nature Biotechnology, 2000The ability to safely and efficiently transfer foreign DNA into cells is a fundamental goal in biotechnology. Toward this end, rapid advances have recently been made in our understanding of mechanisms for DNA stability and transport within cells. Current synthetic DNA delivery systems are versatile and safe, but substantially less efficient than ...
D, Luo, W M, Saltzman
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Polymorphism of a synthetic DNA in solution
Nature, 1976EARLY work on the X-ray diffraction of DNA fibres showed that DNA can adopt different conformations, and up to now at least four different double-helical models have been described in detail: the A, B, C, and D forms1–4. During recent years, it has become likely that DNA in solution also occurs in different conformations5–7.
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Electrotransformation of Highly DNA-RestrictiveCorynebacteriawith Synthetic DNA
Plasmid, 1996Highly DNA-restrictive Corynebacteria can be transformed with DNA made in vitro by PCR amplification of a sequence that contains the replication origin of pBL1, a plasmid common to many Corynebacteria. In all strains examined, the transformation efficiencies of PCR-synthetized DNA equal or improve the performances of heterologous DNA extracted from ...
S, Ankri, O, Reyes, G, Leblon
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1998
Analytical techniques that exploit the phenomenon of sequence-specific hybridization between nucleic acids have been workhorses of modern molecular biology. With the exponentially increasing rate of genomic sequencing has come a need for hybridization techniques that can keep pace with this vast amount of information.
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Analytical techniques that exploit the phenomenon of sequence-specific hybridization between nucleic acids have been workhorses of modern molecular biology. With the exponentially increasing rate of genomic sequencing has come a need for hybridization techniques that can keep pace with this vast amount of information.
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Nature Biotechnology, 2018
An enzymatic approach to oligonucleotide synthesis promises to extend the length and fidelity of synthetic ...
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An enzymatic approach to oligonucleotide synthesis promises to extend the length and fidelity of synthetic ...
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Error Correction Techniques in Synthetic Oligonucleotides and Synthetic DNA
Molecular BiologyMethods for correcting errors in synthetic oligonucleotides and genetic constructs derived from them are described. Methods for isolating oligonucleotides with a perfect structure from oligonucleotide pools are discussed. Methods for correcting errors in the DNA structure using mismatch-specific endonucleases and proteins of the bacterial DNA repair ...
A N, Sinyakov, E V, Kostina
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Topological Transformations of Synthetic DNA Knots
Biochemistry, 1995Two synthetic DNA molecules that can be knotted have been employed as substrates for E. coli DNA topoisomerases I and III. Both molecules contain 104 nucleotides, including sequences that can form two single-turn helical domains, connected by single-stranded oligo(dT) linkers in an X-Y-X'-Y' pairing motif. One of the knots can be ligated to form cyclic
S M, Du +3 more
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Nature Biotechnology, 2010
The complete set of tools needed to synthesize a functional genome and transplant it into a mycoplasma cell opens up the possibility of mixing and matching natural and synthetic DNA to make genomes with new capabilities.
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The complete set of tools needed to synthesize a functional genome and transplant it into a mycoplasma cell opens up the possibility of mixing and matching natural and synthetic DNA to make genomes with new capabilities.
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