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Biosynthesis and preparation of T-2 Toxin, HT-2 Toxin and Neosolantol
Mycotoxin Research, 1987Biosynthesis of trichothecenes by strains ofF. sporotriahioides KF 9 6 and KF 530 was performed on rice as a medium. Three toxins in significant amounts were produced, with yield: T-2 toxin 0.7g from 600g of drv culture, being the highest of the three metabolites, HT-2 toxin 0.06g an Neosolaniol 0.015g.
M, Kostecki +3 more
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Cross-reactivity of antibodies against T-2 with deepoxide T-2 toxin
Mycotoxin Research, 1988Two types of antibodies raised against T-2 toxin, namely anti-T-2-HS-BSA and anti-3 -Ac -NEOS-HS -BSA, showed good cross-reactivity with deepoxy T-2 toxin. Our results indicate that the epoxide is not an important epitope for the production of antibody against T-2 toxin.
R D, Wei, S P, Swanson, F S, Chu
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A monoclonal antibody to T-2 toxin
Letters in Applied Microbiology, 1987A specific, high affinity monoclonal antibody to T-2 toxin of Fusarium has been produced. The monoclonal antibody was conjugated to horse-radish peroxidase and employed to develop a direct competitive enzyme-linked immunosorbent assay (ELISA) for the toxin. The sensitivity of the ELISA was 10 ng/ml with a working range up to 500 ng/ml.
I. A. GOODBRAND +2 more
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A yeast bioassay for T-2 toxin
Journal of Microbiological Methods, 1984Abstract A disk diffusion type bioassay was developed for T-2 toxin using the yeast Kluyveromyces fragilis . The lower limit of detection for this in 0.2 μg of T-2 toxin. The growth of this yeast was sensitive to other trichothecenes such as verrucarin A (0.01 μg).
K.T. Schappert, G.G. Khachatourians
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HPLC: A Tool for the Analysis of T-2 Toxin and HT-2 Toxin in Cereals
Journal of Analytical Toxicology, 1984An analytical procedure for the determination of trichothecenes in various cereals is described. HPLC was performed with a reversed-phase (C18) column eluted with methanol:water (60:40, v/v). Compounds were detected with a refractive index detector. The elution patterns of free and contaminated samples were compared.
R, Schmidt, K, Dose
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Toxicological Features of T-2 Toxin and Related Trichothecenes
Toxicological Sciences, 1984Toxicological characteristics of T-2 toxin and related trichothecenes, mycotoxins produced by Fusarium, Trichoderma, Verrucaria, and others, were investigated in regard to LD50 values, dermal toxicity, hematological changes, and tumorigenicity. The LD50 values (mg/kg) of T-2 toxin in adult male mice were po 10.5, ip 5.2, sc 2.1, and iv 4.2, and those ...
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A Proposed Immunoassay for T-2 Toxin
1990Previously (Uneo, 1983; Dashek et al., 1985; Lacey, 1985) reviewed the evidence regarding the alleged utilization of certain trichothecenes as biological warfare agents and the biological effects and chemistry of the trichothecenes as well as the fungi which produce them.
Charles E. O’Rear +3 more
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Synthesis of radiolabeled T-2 toxin
Journal of Agricultural and Food Chemistry, 1977E M, Wallace +4 more
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[T-2 toxin: occurrence and detection].
Ceska a Slovenska farmacie : casopis Ceske farmaceuticke spolecnosti a Slovenske farmaceuticke spolecnosti, 2007The paper is focused on the occurrence and methods for the detection of T-2 toxin, one of the most toxic trichothecene Fusarium mycotoxin. Due to its physical-chemical properties and high toxicity, T-2 toxin is classified as a potential biological warfare agent.
V, Dohnal, A, Jezková, K, Kuca, D, Jun
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Biology and evolution of bacterial toxin–antitoxin systems
Nature Reviews Microbiology, 2022Nathan Fraikin +2 more
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