Results 101 to 110 of about 1,311,167 (255)

The evolution of creaky voice use in read speech by native-French and native-English speakers in tandem: a pilot study

open access: yesAnglophonia, 2019
Creaky voice is a non-modal (non-neutral) speech feature occurring both at the segmental and suprasegmental levels of languages. It also performs paralinguistic, stylistic, sociolinguistic and extra-linguistic functions. It has been reported as prevalent
Claire Pillot-Loiseau   +3 more
doaj   +1 more source

Challanges of the TanDEM-X Commissioning Phase [PDF]

open access: yes, 2010
The paper describes the execution of the TanDEM-X commissioning phase. It provides the challanges and constraints of the different tasks and their impact on each other during the commissioning phase.
Bachmann, Markus, Hofmann, Harald
core  

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

open access: yesFEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen   +9 more
wiley   +1 more source

Collaborative reading and foreign language learning with literature

open access: yesJournal of Virtual Exchange
Teletandem is a virtual exchange context designed by Telles and Vassallo (2006), in which two learners of different foreign languages meet in video conferencing platforms so that one helps the other in their learning process.
Vanessa Matiola
doaj   +1 more source

Synthesis of 2-trifluoromethylpyrazolo[5,1-a]isoquinolines via silver triflate-catalyzed or electrophile-mediated one-pot tandem reaction

open access: yesBeilstein Journal of Organic Chemistry, 2014
An efficient one-pot tandem cyclization/[3 + 2] cycloaddition reaction of N’-(2-alkynylbenzylidene)hydrazides with ethyl 4,4,4-trifluorobut-2-ynoate under silver triflate-catalyzed or electrophile-mediated conditions is described.
Xiaoli Zhou   +5 more
doaj   +1 more source

Quantitative proteomic analysis reveals different characteristics of bladder cancer cells after exposure to bisphenol A

open access: yesFEBS Open Bio, EarlyView.
Bisphenol A (BPA), a common chemical in plastics, exerts dual effects on bladder cancer cells: low doses promote growth and migration, while high doses suppress growth and migration. Multi‐omics and bioinformatics reveal BPA acts via MAPK and inflammatory pathways.
Shaomin Niu   +10 more
wiley   +1 more source

YlmG1 is localized exclusively to the chloroplast envelope membrane and is involved in preprotein translocation in Arabidopsis thaliana

open access: yesFEBS Open Bio, EarlyView.
Cytosolically synthesized chloroplast preproteins are translocated across the outer and inner envelope membranes through translocons called TOC and TIC, respectively. In green algae and plants, the TIC core is composed of essential membrane proteins, Tic12, Tic20, and Tic214.
Mengyi Li, Xueyang Zhao, Masato Nakai
wiley   +1 more source

Metal halide perovskite: a game-changer for photovoltaics and solar devices via a tandem design

open access: yesScience and Technology of Advanced Materials, 2018
Multi-junction tandem design has been proven to be an effective means to further improve the efficiency of solar cells. However, its share in the photovoltaics market at present is tiny, since the most efficient tandem device comprises III-V ...
Heping Shen   +8 more
doaj   +1 more source

Mass spectrometry based identification of AMP‐O‐Tris generated by Thermococcus onnurineus Cas10

open access: yesFEBS Open Bio, EarlyView.
Isolated Thermococcus onnurineus Cas10 generates the noncanonical ATP‐derived product AMP‐O‐Tris while in Tris‐containing buffer as identified via mass spectrometry, revealing relaxed nucleophile selectivity under isolated conditions. These findings suggest that multiprotein Csm complex assembly restricts Cas10 reactivity toward canonical cyclic ...
Su‐Jin Lee   +6 more
wiley   +1 more source

Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens

open access: yesFEBS Open Bio, EarlyView.
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee   +3 more
wiley   +1 more source

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