Results 1 to 10 of about 666,195 (194)
Bst polymerase — a humble relative of Taq polymerase
Computational and Structural Biotechnology Journal, 2023 DNA polymerases are a superfamily of enzymes synthesizing DNA using DNA as a template. They are essential for nucleic acid metabolism and for DNA replication and repair.
Igor Oscorbin, Maxim Filipenko
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Optimizing Taq polymerase concentration for improved signal-to-noise in the broad range detection of low abundance bacteria. [PDF]
PLoS ONE, 2009 BACKGROUND:PCR in principle can detect a single target molecule in a reaction mixture. Contaminating bacterial DNA in reagents creates a practical limit on the use of PCR to detect dilute bacterial DNA in environmental or public health samples.
Rudolph Spangler +2 more
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A simple and efficient method for extraction of Taq DNA polymerase
Electronic Journal of Biotechnology, 2015 Background: Thermostable DNA polymerase (Taq Pol Ι) from Thermus aquaticus has been widely used in PCR, which was usually extracted with Pluthero's method.
Sique Chen +5 more
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Single-molecule Taq DNA polymerase dynamics [PDF]
Science Advances, 2022 Taq DNA polymerase functions at elevated temperatures with fast conformational dynamics—regimes previously inaccessible to mechanistic, single-molecule studies. Here, single-walled carbon nanotube transistors recorded the motions of Taq molecules processing matched or mismatched template–deoxynucleotide triphosphate pairs from 22° to 85°C.
Mackenzie W. Turvey +12 more
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Instant Taq: Rapid Autoinducible Expression and Chromatography-free Purification of Taq polymerase [PDF]
bioRxiv, 2021 AbstractDNA modifying enzymes are ubiquitous reagents in synthetic biology. Producing these enzymes often requires large culture volumes, purified nucleases and chromatographic separations to make enzymes of necessary quality. We sought to leverage synthetic biology tools to develop engineered strains allowing for not only the production but rapid ...
Romel Menacho-Melgar +2 more
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High frequency of Machado-Joseph disease identified in Southeastern Chinese kindreds with spinocerebellar ataxia [PDF]
BMC Medical Genetics, 2010 Background Machado-Joseph disease (MJD), caused by a CAG repeat expansion located in exon10 of the ATXN3 gene, is now regarded as one of the most common spinocerebellar ataxia (SCA) in the world.
Gan Shi-Rui +8 more
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Frontiers in Bioengineering and Biotechnology, 2021 A change of an aspartic acid to asparagine of Taq (Thermus aquaticus) DNA polymerase is a gain of function mutation that supports faster PCR: the extension times for PCR amplification can be 2–3 times shorter.
Wayne M. Barnes +3 more
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Proceedings of the National Academy of Sciences of the United States of America, 2022 Significance We describe a highly multiplex PCR approach that can identify 10-fold more targets in current real-time PCR assays without additional enzymes or separate reactions.
Qiuying Huang +8 more
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dUTPs conjugated with zwitterionic Cy3 or Cy5 fluorophore analogues are effective substrates for DNA amplification and labelling by Taq polymerase [PDF]
Nucleic Acids Research, 2018 To develop structural modifications of dNTPs that are compatible with Taq DNA polymerase activity, we synthesized eight dUTP derivatives conjugated with Cy3 or Cy5 dye analogues that differed in charge and charge distribution throughout the fluorophore ...
O. A. Zasedateleva +9 more
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Identification and elimination of DNA sequences in Taq DNA polymerase [PDF]
Journal of Clinical Microbiology, 1994 This study confirms that different preparations of Taq DNA polymerase are contaminated with eubacterial DNA. The contaminants appeared to represent more than one strain or species but were not identified as Thermus aquaticus or Escherichia coli. Differences in microcentrifuge tube composition appeared to affect elimination of the contaminants.
M S Hughes, L A Beck, Robin Skuce
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