Results 1 to 10 of about 819,635 (326)
Optimizing Taq Polymerase Concentration for Improved Signal-to-Noise in the Broad Range Detection of Low Abundance Bacteria [PDF]
PLoS ONE, 2009 BACKGROUND:PCR in principle can detect a single target molecule in a reaction mixture. Contaminating bacterial DNA in reagents creates a practical limit on the use of PCR to detect dilute bacterial DNA in environmental or public health samples.
A Heininger +30 more
core +19 more sources
Bst polymerase — a humble relative of Taq polymerase [PDF]
Computational and Structural Biotechnology Journal, 2023 DNA polymerases are a superfamily of enzymes synthesizing DNA using DNA as a template. They are essential for nucleic acid metabolism and for DNA replication and repair. Modern biotechnology and molecular diagnostics rely heavily on DNA polymerases in analyzing nucleic acids.
Igor Oscorbin, Maxim Filipenko
doaj +6 more sources
Single-molecule Taq DNA polymerase dynamics [PDF]
Science Advances, 2022 Taq DNA polymerase functions at elevated temperatures with fast conformational dynamics—regimes previously inaccessible to mechanistic, single-molecule studies. Here, single-walled carbon nanotube transistors recorded the motions of Taq molecules processing matched or mismatched template–deoxynucleotide triphosphate pairs from 22° to 85°C.
Mackenzie W. Turvey +12 more
core +7 more sources
Instant Taq: Rapid Autoinducible Expression and Chromatography-free Purification of Taq polymerase [PDF]
bioRxiv, 2021 AbstractDNA modifying enzymes are ubiquitous reagents in synthetic biology. Producing these enzymes often requires large culture volumes, purified nucleases and chromatographic separations to make enzymes of necessary quality. We sought to leverage synthetic biology tools to develop engineered strains allowing for not only the production but rapid ...
Romel Menacho-Melgar +2 more
openaire +3 more sources
High frequency of Machado-Joseph disease identified in Southeastern Chinese kindreds with spinocerebellar ataxia [PDF]
BMC Medical Genetics, 2010 Background Machado-Joseph disease (MJD), caused by a CAG repeat expansion located in exon10 of the ATXN3 gene, is now regarded as one of the most common spinocerebellar ataxia (SCA) in the world.
Gan Shi-Rui +8 more
core +3 more sources
Global conformations, hydrodynamics, and x-ray scattering properties of Taq and Escherichia coli DNA polymerases in solution [PDF]
, 2003 Escherichia coli polymerase 1 (Pol 1) and Thermus aquaticus Taq polymerase are homologous Type I DNA polymerases, each comprised of a polymerase domain, a proofreading domain (inactive in Taq), and a 5′ nuclease domain.
Byrd, Angela S. +2 more
core +4 more sources
Genomic footprinting with Taq polymerase [PDF]
Nature, 1989 The linear amplification of chemically sequenced DNA with DNA polymerase from Thermus aquaticus permits the direct detection of DNA methylation and protein-DNA interaction sites in vivo.
H. P. Saluz, J. P. Jost
openaire +3 more sources
Taq DNA polymerase blockage at pyrimidine dimers [PDF]
Nucleic Acids Research, 1996 Nucleic Acids Research, 24 (8)
Wellinger, Ralf-Erik, Thoma, Fritz
openaire +5 more sources
Rapid amplification of the RM-Yplex assay [PDF]
, 2016 A multiplex PCR assay consisting of 13 Rapidly Mutating Y STR loci called RM-Yplex was previously developed. Platinum® Taq DNA polymerase was used to amplify the 13 Y STR loci in a single reaction at an amplification time of approximately 2.5 h. In order
Abuidrees, A, Alghafri, A, Hadi, S
core +1 more source
Preparation of Taq DNA Polymerase [PDF]
BIO-PROTOCOL, 2011 1. Dialysis tubing cellulose membrane avg. flat width 33 mm (Sigma-Aldrich, catalog number: D9652) 2. E.coli BL21 strain 3. Ammonium sulfate (Sigma-Aldrich, catalog number: A4418) 4. IPTG 5. Ampicillin 6. (NH4)2SO4 7. 1 M dextrose 8. 1 M Tris HCl (pH 7.9) 9. 0.5 M EDTA (pH 8.0) 10. KCl 11. PMSF 12. Tween 20 13. NP40 14. Lysozyme 15. Glycerol 16. Triton-
openaire +1 more source