Satellite DNA as a target for TaqMan real-time PCR detection of the pinewood nematode, Bursaphelenchus xylophilus [PDF]
The pinewood nematode (PWN), Bursaphelenchus xylophilus , is a major pathogen of conifers, which impacts on forest health, natural ecosystem stability and international trade. As a consequence, it has been listed as a quarantine organism in Europe.
BOONHAM, NEIL+9 more
core +1 more source
Data of self-made Taq DNA polymerase prepared for screening purposes
DNA analysis is a key procedure in genetic engineering. Nowadays the analysis is often done by PCR with Taq DNA polymerase. Although the last enzyme price is quite low, demand for numerous analyses results in much money expenditure which are not ...
E.V. Konovalova+4 more
doaj +1 more source
Demonstration of the Presence of the "Deleted" MIR122 Gene in HepG2 Cells [PDF]
MicroRNA 122 (miR-122) is highly expressed in the liver where it influences diverse biological processes and pathways, including hepatitis C virus replication and metabolism of iron and cholesterol.
Fei, Y+8 more
core +1 more source
Natural polymorphism in the thrombospondin-related adhesive protein of Plasmodium falciparum [PDF]
We have developed a typing system using natural sequence variation in the thrombospondin-related adhesive protein (TRAP) gene of Plasmodium falciparum. This method permits a haplotype to be assigned to any particular TRAP gene.
Bosman, Andrea+9 more
core +1 more source
Aspergillus westerdijkiae polyketide synthase gene “aoks1” is involved in the biosynthesis of ochratoxin A [PDF]
OchratoxinA (OTA) is a potential nephrotoxic, teratogenic, immunogenic, hepatotoxic and carcinogenic mycotoxin, produced by Aspergillus westerdijkiae NRRL 3174.
Atoui, Ali+4 more
core +1 more source
Phage ϕC2 mediates transduction of Tn6215, encoding erythromycin resistance, between Clostridium difficile strains [PDF]
UNLABELLED: In this work, we show that Clostridium difficile phage ϕC2 transduces erm(B), which confers erythromycin resistance, from a donor to a recipient strain at a frequency of 10(-6) per PFU.
Chang, Barbara J+5 more
core +3 more sources
Polymerase chain reaction (PCR) is a powerful molecular biology assay for gene detection and quantification. Conventional DNA primers for PCR often suffer from poor sensitivity in specific gene detection.
Yi-Chen Tsai+2 more
doaj
Genomic resource development for a diploid mint: Mentha longifolia [PDF]
This research project aimed to develop genomic resources needed to enable construction of a genetic linkage map of the diploid mint species Mentha longifolia.
Hadadian, Zahra
core +1 more source
Specific binding of the methyl binding domain protein 2 at the BRCA1-NBR2 locus [PDF]
The methyl-CpG binding domain (MBD) proteins are key molecules in the interpretation of DNA methylation signals leading to gene silencing. We investigated their binding specificity at the constitutively methylated region of a CpG island containing the ...
Auriol, Emilie+3 more
core +2 more sources
Inhibition of Taq DNA polymerase by catalpol.
Fil: Ciuffo, Gladys Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas
Pungitore, Carlos Rodolfo+4 more
openaire +2 more sources