Results 91 to 100 of about 279,379 (333)

Bayesian inference of polymerase dynamics over the exclusion process [PDF]

open access: yesarXiv, 2021
Transcription is a complex phenomenon that permits the conversion of genetic information into phenotype by means of an enzyme called RNA polymerase, which erratically moves along and scans the DNA template. We perform Bayesian inference over a paradigmatic mechanistic model of non-equilibrium statistical physics, i.e., the asymmetric exclusion ...
arxiv  

Small RNA Toxin‐Assisted Evolution of GC‐Preferred ErCas12a for Enhanced Genome Targeting Range

open access: yesAdvanced Science, EarlyView.
ErCas12a is engineered to target GC‐rich PAMs using a small RNA toxin‐aided positive screening system. The resulting variant, enErCas12a, exhibits an expanded PAM profile and facilitates efficient gene editing in both bacterial and mammalian cells, while preserving high targeting specificity for both canonical and non‐canonical PAM targets.
Zehua Chen   +8 more
wiley   +1 more source

Optimization of the Production and Purification of Taq Polymerase Enzyme Containing N666E Mutation in its O-Helix Region

open access: yesمجله دانشکده پزشکی اصفهان, 2012
Background: The aim of this project was to isolate and purify the highly active recombinant Taq DNA polymerase from the strain of Escherichia coli BL21.
Hamid Mirmohammad Sadeghi   +3 more
doaj  

Distinguishing low frequency mutations from RT-PCR and sequence errors in viral deep sequencing data [PDF]

open access: yes, 2015
There is a high prevalence of coronary artery disease (CAD) in patients with left bundle branch block (LBBB); however there are many other causes for this electrocardiographic abnormality.
Haydon, Daniel T.   +6 more
core   +2 more sources

First-passage problems in DNA replication: effects of template tension on stepping and exonuclease activities of a DNA polymerase motor [PDF]

open access: yesJournal of Physics: Condensed Matter, vol.25, 374105 (2013), 2013
A DNA polymerase (DNAP) replicates a template DNA strand. It also exploits the template as the track for its own motor-like mechanical movement. In the polymerase mode it elongates the nascent DNA by one nucleotide in each step. But, whenever it commits an error by misincorporating an incorrect nucleotide, it can switch to an exonuclease mode.
arxiv   +1 more source

AlphaFold‐Guided Bespoke Gene Editing Enhances Field‐Grown Soybean Oil Contents

open access: yesAdvanced Science, EarlyView.
An AlphaFold‐guided method is developed to functionally optimize soybean sugar transporters and achieve bespoke gene editing of GmSWEET10a/b to improve oil content in an elite soybean cultivar in multi‐year, multi‐site field trials. The combination of AI‐guided protein design and gene editing may unlock a huge potential to improve the genetic trait de ...
Jie Wang   +22 more
wiley   +1 more source

Optimization of degenerate oligonucleotide primed PCR for amplification of microdissected chromosome segments [PDF]

open access: yes, 1994
The degenerate oligonucleotide primer of sequence, 5\u27 CCGACTCGAGNNNNNNATGTGG 3\u27, contains a specific 3\u27 hexanucleotide , a central random hexanucleotide and an Xhol site towards it\u27s 5\u27 end.
Mahjoubi, Frouzandeh
core   +1 more source

Human PrimPol is a highly error-prone polymerase regulated by single-stranded DNA binding proteins [PDF]

open access: yes, 2014
PrimPol is a recently identified polymerase involved in eukaryotic DNA damage tolerance, employed in both re-priming and translesion synthesis mechanisms to bypass nuclear and mitochondrial DNA lesions.
Aaron Ehlinger   +55 more
core   +1 more source

On the relationship between logarithmic TAQ and logarithmic THH [PDF]

open access: yesarXiv, 2020
We provide a new description of logarithmic topological Andr\'e-Quillen homology in terms of the indecomposables of an augmented ring spectrum. The new description allows us to interpret logarithmic TAQ as an abstract cotangent complex, and leads to an base-change formula for logarithmic topological Hochschild homology.
arxiv  

Global model categories and topological André-Quillen cohomology [PDF]

open access: yesarXiv, 2023
We introduce global model categories as a general framework to capture several phenomena in global equivariant homotopy theory. We then construct genuine stabilizations of these, generalizing the usual passage from unstable to stable global homotopy theory.
arxiv  

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