Results 91 to 100 of about 166,603 (275)
Improving sensitivity of single tube nested PCR to detect fastidious microorganisms
Heliyon, 2020 Single Tube Nested PCR (ST-nPCR) is of value to clinical laboratories with limited settings for the detection of fastidious microorganisms. The detection sensitivity of ST-nPCR is dependent on ensuring minimal leftovers of outer primers during the second
Diala Shatleh-Rantisi +2 more
doaj +1 more source
Connexin43 Deficiency Leads to Ventricular Arrhythmias by Reprogramming Proline Metabolism
Advanced Science, EarlyView.The study demonstrated that connexin43 (Cx43) knockout caused arrhythmic phenotype and decreased proline content in vitro and in vivo. Mechanistically, Cx43 interacts with the amino acid transporter SNAT2 (sodium‐dependent neutral amino acid transporter), and its deficiency disrupts proline transport and metabolism.
Hangying Ying +8 more
wiley +1 more source
Advanced Science, EarlyView.The translation factor Eukaryotic translation initiation factor 5A (eIF5A) is essential for male fertility in mice. It supports the translation of proteins crucial for heterochromatin organization and acrosome formation. eIF5A deficiency disrupts chromocenter integrity, increases chromatin accessibility, and causes transcriptional dysregulation ...
Yuling Cai +15 more
wiley +1 more source
Advanced Science, EarlyView.This study develops a hierarchically porous hydrogel patch strategy (HPMP), based on gas‐shearing microfluidics and an aqueous two‐phase system to fabricate porous microgels as microgel‐based bioinks. The porous microgels with controllable porous structure exhibit excellent cellular behavior.
Ziyang Liu +13 more
wiley +1 more source
Сибирский онкологический журнал, 2018 PCR clamping/wild-type blocking PCR with non-extendable locked nucleic acid (LNA) oligonucleotides is used for sensitive detection of somatic mutations in tumors.
V. A. Shamanin +4 more
doaj +1 more source
Molecular Dynamics Simulation Trajectories of the Taq RNA Polymerase Holoenzyme
, 2021 Ilona Christy Unarta
openalex +1 more source
PCR with Taq DNA Polymerase with Standard Taq Buffer(M0273) v2
, 2020 This Protocol explains methods for Taq DNA Polymerase with Standard Taq Buffer (M0273).
openaire +1 more source
Advanced Science, EarlyView.This study demonstrates that the imbalance between osteogenic and adipogenic differentiation in senescent BMSCs, leading to excessive adipocyte accumulation, which subsequently impairs bone regeneration in aged mice. To address this pathological dysregulation, a novel energy‐supplying hydrogel system (PBR) has been developed to restore balanced ...
Zirui He +7 more
wiley +1 more source
Hub genes and diagnostic model associated with mitochondrial function in Alzheimer's disease
Animal Models and Experimental Medicine, EarlyView.Alzheimer's disease (AD) is the most common neurodegenerative disorder, and mitochondrial dysfunction has been confirmed in AD patients and mouse models. However, the pathogenic genes associated with AD and early diagnostic methods based on mitochondrial function remain to be explored.
Xuchao Zhu, Ling Zhang, Chuan Qin
wiley +1 more source
Taq-Polymerase Stop Assay to Determine Target Selectivity of G4 Ligands in Native Promoter Sequences of MYC, TERT, and KIT Oncogenes. [PDF]
Pharmaceuticals (Basel), 2023 Chashchina GV +3 more
europepmc +1 more source