Effects of Superparamagnetic Nanoparticle Clusters on the Polymerase Chain Reaction
Applied Sciences, 2012 The polymerase chain reaction (PCR) method is widely used for the reproduction and amplification of specific DNA segments, and a novel PCR method using nanomaterials such as gold nanoparticles has recently been reported. This paper reports on the effects
Toshiaki Higashi +7 more
doaj +1 more source
Application of PCR-DGGE in determining food origin : cases studies of fish and fruits [PDF]
, 2008 The determination of geographical origin is a demand of the traceability system of import-export food products. For this purpose, molecular techniques employing 16S, 26S rDNA profiles generated by PCR-DGGE (polymerase chain reaction - denaturing gradient
Condur, Ana +5 more
core
Genetic diversity among viruses associated with sugarcane mosaic disease in Tucumán, Argentina [PDF]
, 2009 Sugarcane leaves with mosaic symptoms were collected in 2006--07 in Tucumán (Argentina) and analyzed by reverse-transcriptase polymerase chain reaction (RT-PCR) restriction fragment length polymorphism (RFLP) and sequencing of a fragment of the Sugarcane
Castagnaro, Atilio Pedro +6 more
core +2 more sources
PCR-RFLP Using BseDI Enzyme for Pork Authentication in Sausage and Nugget Products [PDF]
, 2011 A polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) using BseDI restriction enzyme had been applied for identifying the presence of pork in processed meat (beef sausage and chicken nugget) including before and after frying ...
Abidin, M. Z. (M) +3 more
core
Labeling of Unique Sequences in Double-Stranded DNA at Sites of Vicinal Nicks Generated by Nicking Endonucleases [PDF]
, 2008 We describe a new approach for labeling of unique sequences within dsDNA under nondenaturing conditions. The method is based on the site-specific formation of vicinal nicks, which are created by nicking endonucleases (NEases) at specified DNA sites on ...
Frank-Kamenetskii, Maxim D., Kuhn, Heiko
core +3 more sources
Thermodynamic and Structural Origins for the Thermostability of Taq DNA Polymerase
The FASEB Journal, 2009 The thermal stability of Taq DNA polymerase is well known, and is the basis for its use in PCR. We have previously shown that at 25oC, Klentaq polymerase (the large fragment domain of Taq) has a folding free energy (δGfold) of ‐27 kcal/mole, making it one of the most stable monomeric proteins yet characterized.
Vince J. LiCata +2 more
openaire +2 more sources
Contribution of Taq polymerase-induced errors to the estimation of RNA virus diversity.
Journal of General Virology, 1998 The genetic diversity of a vesicular stomatitis virus population was analysed by RT-PCR, cloning and sequencing of two approximately 500 nucleotide regions of the virus genome.
M. Bracho, Andrés Moya, Eladio Barrio
semanticscholar +1 more source
Scientific ReportsThe development and characterization of a new enzyme reaction contribute to advancements in modern biotechnology. Here, we report a novel CIS (clamping-mediated incorporation of single-stranded DNA with concomitant DNA synthesis) reaction catalyzed by ...
Yoshiyuki Ohtsubo +2 more
doaj +1 more source
DGR mutagenic transposition occurs via hypermutagenic reverse transcription primed by nicked template RNA. [PDF]
, 2017 Diversity-generating retroelements (DGRs) are molecular evolution machines that facilitate microbial adaptation to environmental changes. Hypervariation occurs via a mutagenic retrotransposition process from a template repeat (TR) to a variable repeat ...
Guo, Huatao +6 more
core +3 more sources
Development of a nucleic acid lateral flow immunoassay for simultaneous detection of Listeria spp. and Listeriamonocytogenes in food [PDF]
, 2009 We present a new nucleic acid lateral flow immunoassay (NALFIA) for the assessment of listeria contamination. The detection procedure starts with enrichment of sample in Half Fraser broth (24 h). Following isolation of DNA, a duplex PCR is performed with
Amerongen, A., van +3 more
core +2 more sources