Results 111 to 120 of about 819,635 (326)

Effects of Superparamagnetic Nanoparticle Clusters on the Polymerase Chain Reaction

Applied Sciences, 2012
The polymerase chain reaction (PCR) method is widely used for the reproduction and amplification of specific DNA segments, and a novel PCR method using nanomaterials such as gold nanoparticles has recently been reported. This paper reports on the effects
Toshiaki Higashi, Hiroaki Minegishi, Yutaka Nagaoka, Takahiro Fukuda, Akinobu Echigo, Ron Usami, Toru Maekawa, Tatsuro Hanajiri   +7 more
doaj   +1 more source

Structure and possible function of a G-quadruplex in the long terminal repeat of the proviral HIV-1 genome [PDF]

, 2016
open8The long terminal repeat (LTR) of the proviral human immunodeficiency virus (HIV)-1 genome is integral to virus transcription and host cell infection.

core   +1 more source

Flavonifractor Plautii or Its Metabolite Desaminotyrosine as Prophylactic Agents for Alleviating Myocardial Ischemia/Reperfusion Injury

Advanced Science, EarlyView.
The gut microbiome is a potential target for treating cardiovascular disease. It is found that prophylactic administration of Flavonifractor plautii or its metabolite desaminotyrosine (DAT) exerts pleiotropic cardioprotective effects via promoting cardiomyocyte survival and attenuating cardiac inflammation against myocardial ischemia/reperfusion (I/R ...
Heng Du, Xu Liu, Jianghua Shen, Hailong Yuan, Hao Zhang, Gan Xi, Yujing Li, Yuhan Wang, Jiahe Zhang, Chaofan Yang, Pengfei Xu, Jiawan Wang, Fang Wang, Siqi Liu, Yanan Zhou, Qi Gu, Jingjing Lu, Tuo Wei, Zeyu Gao, Jingyi Zang, Jun Wang, Moshi Song   +21 more
wiley   +1 more source

Molecular effects of site-specific phosphate-methylated primer on the structure and motions of Taq DNA polymerase

Computational and Structural Biotechnology Journal, 2023
Polymerase chain reaction (PCR) is a powerful molecular biology assay for gene detection and quantification. Conventional DNA primers for PCR often suffer from poor sensitivity in specific gene detection.
Yi-Chen Tsai, Wen-Yih Chen, Chi-cheng Chiu   +2 more
doaj  

Efficient DNA isolation from moroccan arar tree [Tetraclinis articulata (Vahl) Masters] leaves and optimization of the rapd-pcr molecular technique [PDF]

, 2010
Efficient DNA isolation from Moroccan Arar tree [Tetraclinis articulata (Vahl) Masters] leaves and optimization of the RAPD-PCR molecular technique. Molecular genetic analysis of Arar tree [Tetraclinis articulata (Vahl) Masters] is often limited by the
Abourouh, Mohamed, Bakkali Yakhlefb, Salaheddine, Guenoun, Imane, Hamamouch, Noureddine, kerdouh, Benaîssa   +4 more
core   +2 more sources

Rapid Optical Cavity PCR. [PDF]

, 2015
Recent outbreaks of deadly infectious diseases, such as Ebola and Middle East respiratory syndrome coronavirus, have motivated the research for accurate, rapid diagnostics that can be administered at the point of care.
Gustafson, Lars, Haack, Amanda, Hong, SoonGweon, Hoxha, Ori, Lee, Luke, Son, Jun, Song, Minsun   +6 more
core   +2 more sources

AlphaFold‐Guided Bespoke Gene Editing Enhances Field‐Grown Soybean Oil Contents

Advanced Science, EarlyView.
An AlphaFold‐guided method is developed to functionally optimize soybean sugar transporters and achieve bespoke gene editing of GmSWEET10a/b to improve oil content in an elite soybean cultivar in multi‐year, multi‐site field trials. The combination of AI‐guided protein design and gene editing may unlock a huge potential to improve the genetic trait de ...
Jie Wang, Li Zhang, Shoudong Wang, Xin Wang, Suning Li, Pingping Gong, Mengyan Bai, Arnav Paul, Nathan Tvedt, Hengrui Ren, Maoxiang Yang, Zhihui Zhang, Shaodong Zhou, Jiayi Sun, Xianjin Wu, Huaqin Kuang, Zhenghua Du, Yonghui Dong, Xiaolei Shi, Meina Li, Diwakar Shukla, Long Yan, Yuefeng Guan   +22 more
wiley   +1 more source

Inhibition of Taq DNA polymerase by catalpol.

Cellular and molecular biology (Noisy-le-Grand, France), 2004
Fil: Ciuffo, Gladys Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas
Pungitore, Carlos Rodolfo, Juri Ayub, Maximiliano, Borkowski, Eduardo Jorge, Tonn, Carlos Eugenio, Ciuffo, Gladys Maria   +4 more
openaire   +2 more sources

Minor structural modifications to alchemix influence mechanism of action and pharmacological activity [PDF]

, 2012
Alchemix is an exemplar of a class of anthraquinone with efficacy against multidrug resistant tumors. We have explored further the mechanism of action of alchemix and investigated the effect of extending its side arm bearing the alkylating functionality ...
Abdallah, QMA, Abdel-Rahman, H, Bingham, JP, Cosentino, L, Etzad, J, Hartley, JA, Helleday, D, Johansson, F, Kiakos, K, Patterson, LH, Phillips, Roger, Pors, K, Wheelhouse, RT   +12 more
core   +1 more source

MAGEA6 Engages a YY1‐Dependent Transcription to Dictate Perineural Invasion in Colorectal Cancer

Advanced Science, EarlyView.
This study investigates the role of MAGEA6 in perineural invasion (PNI) in colorectal cancer (CRC). MAGEA6 promotes CRC invasiveness by inhibiting YY1 ubiquitination, enhancing CXCL1 secretion, and recruiting Schwann cells. These findings highlight the potential of targeting the MAGEA6/YY1/CXCL1 axis for therapeutic strategies against PNI and tumor ...
Hao Wang, Kexin He, Ruixue Huo, Weihan Li, Shan Zhang, Lu‐Ju Jiang, Hao Wu, Minhao Yu, Shu‐Heng Jiang, Junli Xue   +9 more
wiley   +1 more source