Results 111 to 120 of about 166,603 (275)
Fhod3 in zebrafish supports myofibril stability during growth of embryonic skeletal muscle
Developmental Dynamics, EarlyView.Abstract Background Actin filament organization in cardiomyocytes critically depends on the formin Fhod3, but a role for Fhod3 in skeletal muscle development has not yet been described. Results We demonstrate here that in zebrafish mutated for one of two fhod3 paralog genes, fhod3a, skeletal muscle of the trunk appears normal through 2 days post ...
Aubrie Russell +3 more
wiley +1 more source
Computer‐Aided Functional Oligosaccharide Screening and the Regulatory Role in Lipid Metabolism
Food Bioengineering, EarlyView.ABSTRACT This study investigated the lipid‐lowering effects and intervention mechanisms of structurally diverse functional oligosaccharides on non‐alcoholic fatty liver disease (NAFLD). By integrating computational tools such as molecular docking and molecular dynamics simulations (MDS), a comprehensive “in silico‐in vitro‐in vivo” tri‐dimensional ...
Rui Jing +3 more
wiley +1 more source
An Improved Transformation System for Molecular Biology Research in Peach Fruit
Future Postharvest and Food, EarlyView.Schematic Diagram of Repeated Agrobacterium‐Mediated Transformation. A triple‐stage on‐tree Agrobacterium injection enables gene manipulation in peach with up to 32‐day expression, supporting efficient, nonheritable functional analysis without tissue culture.
Chunhong Li +7 more
wiley +1 more source
iMetaOmics, EarlyView.First use of a natural swine model with lipid metabolism to directly link lipid differences to a quantifiable intestinal villus height phenotype. Identified the phospholipid‐derived oleoylethanolamide, rather than canonical fatty acids, as the endogenous primary ligand that activates peroxisome proliferator‐activated receptors α (PPARα) to enlarge ...
Qianqian Wang +9 more
wiley +1 more source
iMetaOmics, EarlyView.A new bioinformatics pipeline called ECCFP has been developed to improve the detection of extrachromosomal circular DNA (eccDNA) from long‐read sequencing data. ECCFP uses all consecutive full passes from individual reads for candidate eccDNA identification and consolidates candidate eccDNAs to generate accurate unique eccDNA.
Wang Li +14 more
wiley +1 more source
Switching the activity of Taq polymerase using clamp-like triplex aptamer structure. [PDF]
Nucleic Acids Res, 2020 Hu Y, Wang Z, Chen Z, Pan L.
europepmc +1 more source
Journal of Clinical Laboratory Analysis, EarlyView.A novel hexaplex recombinase polymerase amplification (RPA) method was developed to rapidly and accurately detect five key carbapenemase genes and a Klebsiella pneumoniae‐specific target in a single reaction. This makes it an effective tool for CRKP detection in resource‐constrained environments.
Suthawan Mutitanon +1 more
wiley +1 more source
Periodontitis and high phosphate intake alone or in combination adversely affect the kidney
Journal of Periodontology, EarlyView.Abstract Background Periodontitis and high phosphate (HP) intake can negatively affect the kidney in the presence of renal disease. This study aimed to evaluate the effects of periodontitis or HP intake, either alone or concurrently, on the periodontal tissue and the kidney under normal renal conditions.
Ae Ri Kim +4 more
wiley +1 more source
A-Tailing with Taq Polymerase v1
, 2015 This protocol can be used to add As to the blunt-ends of DNA fragments that have been amplified using a high-fidelity polymerase (such as Q5® High Fidelity DNA Polymerase).
openaire +1 more source
The Kaohsiung Journal of Medical Sciences, EarlyView.ABSTRACT Alveolar epithelial cell pyroptosis exacerbates inflammation and tissue damage by releasing inflammatory mediators, thereby promoting the development of acute lung injury (ALI). However, the fundamental mechanism underlying alveolar epithelial cell pyroptosis in ALI has not yet been elucidated. Lipopolysaccharide (LPS) was used to simulate ALI
Lei Wang +5 more
wiley +1 more source