Results 111 to 120 of about 666,195 (194)

REQUIREMENTS FOR EFFICIENT PCR CLAMPING BY LOCKED NUCLEIC ACID OLIGONUCLEOTIES FOR SIMPLE AND SENSITIVE DETECTION OF SOMATIC MUTATIONS

Сибирский онкологический журнал, 2018
PCR clamping/wild-type blocking PCR with non-extendable locked nucleic acid (LNA) oligonucleotides is used for sensitive detection of somatic mutations in tumors.
V. A. Shamanin, I. V. Karpov, E. E. Pisareva, N. I. Gutkina, S. P. Kovalenko   +4 more
doaj   +1 more source

A robust strategy for overexpression of DNA polymerase from Thermus aquaticus using an IPTG-independent autoinduction system in a benchtop bioreactor

Scientific Reports
The DNA polymerase derived from Thermus aquaticus is the most widely utilized among various DNA polymerases, indicating its significant economic importance. Consequently, efforts to achieve a substantial yield of Taq DNA polymerase (Taq-pol) are ongoing.
Fina Amreta Laksmi, Kenny Lischer, Yudhi Nugraha, Wiga Alif Violando, Helbert, Isa Nuryana, Firyal Nida Khasna, Naswandi Nur, Kharisma Panji Ramadhan, Destrianti Adelina Lumban Tobing, Hariyatun, Iman Hidayat   +11 more
doaj   +1 more source

Rapid one-step automated sequencing reactions for 16 DNA samples using Taq polymerase and fluorescent primers [PDF]

, 1991
T. Ciora, P. Deneèfle, J F Mayaux
openalex   +1 more source

CLONING AND CHARACTERIZATION OF ISOLATED TAQ DNA POLYMERASE GENE FROM PHAGE [PDF]

, 2009
Taq polymerase is a thermo stable DNA polymerase named after the thermophilic bacterium T.aquaticus, having a molecular weight of 66,000-94,000 Daltons. Taq DNA polymerases have become a valuable tool in today’s research. The choice of the DNA polymerase
KUMAR, ANIL, MISHRA, NIKHIL
core   +1 more source

A simplified protocol for producing Taq DNA polymerase in biology laboratory

Research in Molecular Medicine, 2013
Background: Taq DNA polymerase is a very important enzyme for molecular biological studies such as DNA amplification and DNA sequencing by the PCR. It is a standard enzyme that is used in 90% of molecular biology labs today.
Touraj Farazmandfar, Alireza Rafiei, Mohammad Bagher Hashemi-Sotehoh, Reza Valadan, Mohammad Alavi, Fatemeh Moradian   +5 more
doaj  

Random mutagenesis of gene-sized DNA molecules by use of PCR with Taq DNA polymerase [PDF]

, 1991
Yuhong Zhou, X P Zhang, Richard H. Ebright   +2 more
openalex   +1 more source

PCR performance of a thermostable heterodimeric archaeal DNA polymerase

Frontiers in Microbiology, 2014
DNA polymerases are versatile tools used in numerous important molecular biological core technologies like the ubiquitous polymerase chain reaction (PCR), cDNA cloning, genome sequencing and nucleic acid based diagnostics.
Tom eKillelea, Celine eRalec, Audrey eBosse, Ghislaine eHenneke   +3 more
doaj   +1 more source

Simplified template preparation and improved direct sequencing using Taq polymerase. [PDF]

, 1992
Anand Srivastava, V. Montanaro, Juha Kere   +2 more
openalex   +1 more source

Surface mucous as a source of genomic DNA from Atlantic billfishes (Istiophoridae) and swordfish (Xiphiidae) [PDF]

, 2009
Procedures for sampling genomic DNA from live billfishes involve manual restraint and tissue excision that can be difficult to carry out and may produce stresses that affect fish survival.
Bernard, Andrea M., Faugue, Ronald M., Hoolihan, John P., Horn, Rebekah L., Perez, Nerida F., Schultz, Duane R., Snodgrass, Derke   +6 more
core   +1 more source

Affinity maturation of a Taq DNA polymerase specific affibody by helix shuffling [PDF]

, 1999
Elin Gunneriusson, Karin Nord, Mathias Uhlén, Per‐Åke Nygren   +3 more
openalex   +1 more source