Inhibition of Taq DNA polymerase by iridoid aglycone derivates.
Fil: García, Celina E..
Pungitore, Carlos Rodolfo+3 more
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Rapid purification of high-activityTaqDNA polymerase
The method described here is derived from that of Engelke et al. (1), and uses the same cloned form of Thermus aquaticus {Taq) DNA polymerase to produce this enzyme in E.coli. The modified purification method described here is quite simple, however it is important to note that factors such as the bacterial strain used, induction time and protein ...
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PCR with Taq DNA Polymerase (M0273) v1
This PCR Protocol is for Taq DNA Polymerase with Standard Taq Buffer (M0273)
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Reverse transcription polymerase chain reaction (RT-PCR) has evolved as a widely used approach in biotechnology and molecular diagnostics. It represents a powerful tool for amplifying and analysing RNA molecules and has therefore found widespread ...
Luisa B. Huber+4 more
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Substitution of Asn for Ser543 in the large fragment of Taq DNA polymerase increases the efficiency of synthesis of long DNA molecules [PDF]
Konstantin B. Ignatov+2 more
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Identification of a mutation in the para-sodium channel gene of the cattle tick Rhipicephalus (Boophilus) microplus associated with resistance to synthetic pyrethroid acaricides [PDF]
Resistance against synthetic pyrethroid (SP) products for the control of cattle ticks in Australia was detected in the field in 1984, within a very short time of commercial introduction.
Corley, S.W.+5 more
core +1 more source
Taq DNA polymerase slippage mutation rates measured by PCR and quasi-likelihood analysis: (CA/GT)n and (A/T)n microsatellites [PDF]
Dheeraj Shinde
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Insertion of the T3 DNA polymerase thioredoxin binding domain enhances the processivity and fidelity of Taq DNA polymerase [PDF]
J.F. Davidson
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Improving sensitivity of single tube nested PCR to detect fastidious microorganisms
Single Tube Nested PCR (ST-nPCR) is of value to clinical laboratories with limited settings for the detection of fastidious microorganisms. The detection sensitivity of ST-nPCR is dependent on ensuring minimal leftovers of outer primers during the second
Diala Shatleh-Rantisi+2 more
doaj