Results 121 to 130 of about 819,635 (326)

Thermodynamic and Structural Origins for the Thermostability of Taq DNA Polymerase

The FASEB Journal, 2009
The thermal stability of Taq DNA polymerase is well known, and is the basis for its use in PCR. We have previously shown that at 25oC, Klentaq polymerase (the large fragment domain of Taq) has a folding free energy (δGfold) of ‐27 kcal/mole, making it one of the most stable monomeric proteins yet characterized.
Vince J. LiCata, Yanling Yang, Chin-Chi Liu   +2 more
openaire   +2 more sources

Efficient molecular cloning of environmental DNA from geothermal sediments [PDF]

, 2002
An efficient and simple method for constructing an environmental library using mechanically sheared DNA obtained directly from geothermal sediments is presented. The method is based on blunt-end modification of DNA fragments followed by 3' -adenylation
Cowan, Donald A., Jaenicke, Thomas, Wilkinson, Dianne E.   +2 more
core  

A Fragment-Based Approach for the Development of G-Quadruplex Ligands: Role of the Amidoxime Moiety [PDF]

, 2018
G-quadruplex (G4) nucleic acid structures have been reported to be involved in several human pathologies, including cancer, neurodegenerative disorders and infectious diseases; however, G4 targeting compounds still need implementation in terms of drug ...
Butovskaya, Elena, Doria, Filippo, Freccero, Mauro, Lena, Alberto, Nadai, Matteo, Pirota, Valentina, Richter, Sara N, Tassinari, Martina   +7 more
core   +2 more sources

Overcoming Extreme Ammonia Inhibition on Methanogenesis by Artificially Constructing a Synergistically Community with Acidogenic Bacteria and Hydrogenotrophic Archaea

Advanced Science, EarlyView.
This study proposes a two‐step biological strategy to synergistically enhance the three stages of anaerobic digestion, ultimately eliminating the extreme ammonia inhibition and breaking through the inefficiency bottleneck of conventional biotechnology.
Heng Wu, Huaiwen Zhang, Taili Dong, Zhenyu Li, Xiaohui Guo, Heyu Chen, Yiqing Yao   +6 more
wiley   +1 more source

Polymerase-endonuclease amplification reaction for large-scale enzymatic production of antisense oligonucleotide [PDF]

, 2009
Synthetic oligonucleotides are contaminated with highly homologous failure sequences. Oligonucleotide synthesis is difficult to scale up because it requires expensive equipments, hazardous chemicals, and tedious purification process.
Deming Gou, Xiaolong Wang
core   +1 more source

Human PrimPol is a highly error-prone polymerase regulated by single-stranded DNA binding proteins [PDF]

, 2014
PrimPol is a recently identified polymerase involved in eukaryotic DNA damage tolerance, employed in both re-priming and translesion synthesis mechanisms to bypass nuclear and mitochondrial DNA lesions.
Aaron Ehlinger, Aguilera, Aidan J. Doherty, Ananda, Arunkumar, Baptiste, Bianchi, Braun, Byun, Collins, Dennis, Eckert, Engelke, Evans, Fortune, Frank, Friedberg, Furukohri, Garcia-Gomez, Garg, Garg, Heller, Hile, Huang, Indiani, J. Mark Skehel, Jozwiakowski, Keen, Keith, Keller, Kenny, Kristin A. Eckert, Laura J. Bailey, Lehmann, Li, Longley, Maga, Masuda, Mourón, Oliveira, Opresko, Perkins, Rudd, Rudd, Ryan P. Barnes, Sale, Schmidt, Sean G. Rudd, Shcherbakova, Stanislaw K. Jozwiakowski, Thomas A. Guilliam, Toshiki, Voss, Walter J. Chazin, Wan, Zhong   +55 more
core   +1 more source

Accelerating Sono‐Piezoelectric Charge Transport for Antibacterial Therapy and Bone Regeneration by Metal‐Deficient TiO2 Spin‐Polarization Effect

Advanced Science, EarlyView.
The deficient TiO2 (DTO) and Barium titanate (BTO) heterojunction treats osteomyelitis through SDT. The spin‐polarization effect induced by the unpaired spin electrons in Ti atoms can expedite the transfer of ultrasonic piezoelectric charges in BTO, enhancing surface sonocatalytic efficiency.
Chaofeng Wang, Shuilin Wu, Congyang Mao, Liguo Jin, Hanpeng Liu, Yufeng Zheng, Chunyong Liang, Shengli Zhu, Zhaoyang Li, Hui Jiang, Xiangmei Liu   +10 more
wiley   +1 more source

Contribution of Taq polymerase-induced errors to the estimation of RNA virus diversity.

Journal of General Virology, 1998
The genetic diversity of a vesicular stomatitis virus population was analysed by RT-PCR, cloning and sequencing of two approximately 500 nucleotide regions of the virus genome.
M. Bracho, Andrés Moya, Eladio Barrio
semanticscholar   +1 more source

Inhibition of Heat Shock Protein 90β by Catalpol: A Potential Therapeutic Approach for Alleviating Inflammation‐Induced Cartilage Injuries in Osteoarthritis

Advanced Science, EarlyView.
The schematic diagram delineates the chondroprotective effects of Ca‐bMSN in OA models. The upper portion of the diagram depicts the synthesis process of Ca‐bMSN. The lower section illustrates the post‐injection of Ca‐bMSN in joint cavity, catalpol released and subsequently mediates the reduction of oxidative stress, ECM catabolism, apoptosis, and ...
Zhenwei Zhou, Binghua Zhang, Lang Liu, Jie Yang, Yuting Wang, Cheng Lv, He Zhang, Yuchi Wei, Zhanliang Jiang, Zeyu Peng, Daqing Zhao, Xiangyang Leng, Xiangyan Li, Hang Su, Haisi Dong   +14 more
wiley   +1 more source

Commodity Supply and Extraterritorial Patent Infringement in Life Technologies V. Promega [PDF]

, 2017
American patent law grants inventors the exclusive right, within U.S. territory, to make, sell, use, and import their patented inventions. In response to attempts to circumvent the right by making the components of an invention within the U.S.
Powell, G. Edward
core   +1 more source