Results 141 to 150 of about 819,635 (326)
PCR performance of a thermostable heterodimeric archaeal DNA polymerase
Frontiers in Microbiology, 2014 DNA polymerases are versatile tools used in numerous important molecular biological core technologies like the ubiquitous polymerase chain reaction (PCR), cDNA cloning, genome sequencing and nucleic acid based diagnostics.
Tom eKillelea +3 more
doaj +1 more source
Random mutagenesis of gene-sized DNA molecules by use of PCR with Taq DNA polymerase [PDF]
, 1991 Yuhong Zhou +2 more
openalex +1 more source
Biotechnology and Applied Biochemistry, EarlyView.ABSTRACT This work presents the pioneering development of an aptamer‐based electrochemical biosensor for real‐time monitoring of parathyroid hormone (PTH) levels, with a focus on intraoperative assessment during parathyroid surgery. It introduces, for the first time, the selection and characterization of aptamers targeting distinct segments of the PTH ...
Reza Didarian +5 more
wiley +1 more source
A simplified protocol for producing Taq DNA polymerase in biology laboratory
Research in Molecular Medicine, 2013 Background: Taq DNA polymerase is a very important enzyme for molecular biological studies such as DNA amplification and DNA sequencing by the PCR. It is a standard enzyme that is used in 90% of molecular biology labs today.
Touraj Farazmandfar +5 more
doaj
Improving trans‐cinnamic acid production in a model cyanobacterium
Biotechnology Progress, Volume 41, Issue 2, March/April 2025.Abstract trans‐Cinnamic acid (tCA) is a precursor in the synthesis of many high‐value compounds with bio‐active qualities useful in applications like medicine, polymers, and cosmetics. Currently tCA is produced by industrial chemical synthesis from fossil fuels or cost‐prohibitive isolation from terrestrial plants.
Darcy Hunstiger +3 more
wiley +1 more source
Substrate Range and Genetic Analysis of Acinetobacter Vanillate Demethylase [PDF]
, 2000 An Acinetobacter sp. genetic screen was used to probe structure-function relationships in vanillate demethylase, a two-component monooxygenase. Mutants with null, leaky, and heat-sensitive phenotypes were isolated.
Morawski, Birgit +2 more
core
Cancer Communications, EarlyView.Abstract Background Glioblastoma (GBM) is largely refractory to antibodies against programmed cell death 1 (anti‐PD‐1) therapy. Fully understanding the cellular heterogeneity and immune adaptations in response to anti‐PD‐1 therapy is necessary to design more effective immunotherapies for GBM.
Rongrong Zhao +11 more
wiley +1 more source
Rapid one-step automated sequencing reactions for 16 DNA samples using Taq polymerase and fluorescent primers [PDF]
, 1991 T. Ciora, P. Deneèfle, J F Mayaux
openalex +1 more source
Cancer Communications, EarlyView.Abstract Background Pancreatic cancer's aberrant lipid metabolism fuels cell growth, invasion, and metastasis, yet its impact on immune surveillance and immunotherapy is unclear. This study investigated how sterol regulatory element‐binding transcription factor 1 (SREBP1)‐driven lipid metabolism affects the tumor microenvironment (TME) in pancreatic ...
Mengyi Lao +12 more
wiley +1 more source
Simplified template preparation and improved direct sequencing using Taq polymerase. [PDF]
, 1992 Anand Srivastava +2 more
openalex +1 more source