Results 151 to 160 of about 819,635 (326)
Inhibition of Taq DNA polymerase by iridoid aglycone derivates.
Cellular and molecular biology (Noisy-le-Grand, France), 2012 Fil: García, Celina E..
Pungitore, Carlos Rodolfo +3 more
openaire +2 more sources
Clade III Synthases Add Cyclic And Linear Terpenoids To The Psilocybe Metabolome
ChemBioChem, Accepted Article.Psilocybe “magic mushrooms” are best known for their indolethylamine psilocybin, yet they encode enzymes for a much more diverse arsenal of small and potentially bioactive molecules. Here, we report on four Psilocybe cubensis clade III sesquiterpene synthases, CubB‐CubE, whose genes are differently expressed in fruiting bodies compared to vegetative ...
Nick Zschoche +7 more
wiley +1 more source
Substitution of Asn for Ser543 in the large fragment of Taq DNA polymerase increases the efficiency of synthesis of long DNA molecules [PDF]
, 1998 Konstantin B. Ignatov +2 more
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Rapid purification of high-activityTaqDNA polymerase
Nucleic Acids Research, 1993 The method described here is derived from that of Engelke et al. (1), and uses the same cloned form of Thermus aquaticus {Taq) DNA polymerase to produce this enzyme in E.coli. The modified purification method described here is quite simple, however it is important to note that factors such as the bacterial strain used, induction time and protein ...
openaire +4 more sources
Engineered Exosome‐Based Senolytic Therapy Alleviates Stroke by Targeting p21+CD86+ Microglia
Exploration, EarlyView.We identified a harmful immune cell type, p21+CD86+ microglia, driving post‐stroke inflammation and developed a targeted exosome‐based therapy (Que@micro‐Exo) to eliminate these cells. This innovative treatment reduced inflammation, alleviated brain damage, and improved recovery in stroke models, offering a promising strategy for stroke and other ...
Jialei Yang, Shipo Wu, Miao He
wiley +1 more source
The RNA Binding Protein Bcas2 is Required for Antibody Class Switch in Activated‐B Cells
Exploration, EarlyView.Bcas2 recruits DHX15 and SRSF7 to form a protein complex for the splicing of CSR‐related mRNA at the 5' ss and 3' ss, generating two mature mRNA isoforms, which are ultimately translated into CSR‐related proteins. These CSR‐related proteins bind to DNA associated with antibodies, affecting the DSB repair of the relevant DNA after AID induction, thereby
Yu Chen +29 more
wiley +1 more source
Food Safety and Health, EarlyView.Inoculation of VBNC Staphylococcus aureus into foods and detection of VBNC S. aureus in food samples using both Fe3O4‐PMA‐qPCR and Kit‐PMA‐qPCR assays. (A) is the induction and inoculation of VBNC S. aureus into rice, chicken, and milk. (B) is the detection procedure using the Fe3O4‐PMA‐qPCR assay.
Yong Wu +5 more
wiley +1 more source
Affinity maturation of a Taq DNA polymerase specific affibody by helix shuffling [PDF]
, 1999 Elin Gunneriusson +3 more
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PCR with Taq DNA Polymerase (M0273) v1
, 2014 This PCR Protocol is for Taq DNA Polymerase with Standard Taq Buffer (M0273)
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iLABMED, EarlyView.We developed an on‐site detection platform for rapid detection of Mycobacterium tuberculosis complex (MTBC) based on the IS1081 gene. The platform utilizes real‐time multiplex recombinase polymerase amplification (mRPA) to achieve a reaction time of less than 30 min.
Xiaoyan Tan +5 more
wiley +1 more source