Results 161 to 170 of about 666,195 (194)
Presence of β-Lactamase Gene TEM-1 DNA Sequence in Commercial Taq DNA Polymerase
, 2005 Chuen‐Sheue Chiang +4 more
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Springer Reference Medizin, 1989
The polymerase chain reaction (PCR) method for amplifying selectively discrete segments of DNA has found wide-spread applications in molecular biology, due in part, to the substitution of a thermostable DNA polymerase isolated from Thermus aquaticus (Taq)1 for the previously used E.
D. Gelfand
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The polymerase chain reaction (PCR) method for amplifying selectively discrete segments of DNA has found wide-spread applications in molecular biology, due in part, to the substitution of a thermostable DNA polymerase isolated from Thermus aquaticus (Taq)1 for the previously used E.
D. Gelfand
openaire +3 more sources
Structure of Taq polymerase with DNA at the polymerase active site
Nature, 1996The DNA polymerase from Thermus aquaticus (Taq polymerase) is homologous to Escherichia coli DNA polymerase I (Pol I) and likewise has domains responsible for DNA polymerase and 5' nuclease activities. The structures to the polymerase domains of Taq polymerase and of the Klenow fragment (KF) of Pol I are almost identical, whereas the structure of a ...
Jimin Wang +3 more
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NaOH Treatment to Neutralize Inhibitors of Taq Polymerase
Journal of Forensic Sciences, 1999Abstract The introduction of polymerase chain reaction (PCR) into the forensic field has greatly extended the ability to analyze DNA from small or degraded samples. However, one significant problem with PCR analysis is the sensitivity of Taq Polymerase to inhibitors found in many substrates commonly encountered with evidentiary materials.
Carol A. Scherczinger +3 more
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Taq DNA Polymerase Mutants and 2′-Modified Sugar Recognition
Biochemistry, 2015Chemical modifications to DNA, such as 2' modifications, are expected to increase the biotechnological utility of DNA; however, these modified forms of DNA are limited by their inability to be effectively synthesized by DNA polymerase enzymes.
Aaron M. Leconte +9 more
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Inhibition of Taq polymerase as a method for screening heparin for oversulfated contaminants
Biomaterials, 2008Heparin and low molecular heparins are extensively used in the treatment of a wide range of diseases in addition to their classic anticoagulant activity and can be found coating medical devices such as catheters, stents and filters. Early in 2008, a sharp increase in heparin-associated severe adverse events, including over 80 deaths, was linked to the ...
Cecilia Tami +6 more
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Primer/Template-Independent Synthesis of Poly d(A-T) by Taq Polymerase
Biochemical and Biophysical Research Communications, 1997Taq DNA polymerase polymerized dATP and dTTP to poly d(A-T) without requiring added primer/template in the temperature range of 60-70 degrees C. Tth DNA polymerase also catalyzed the reaction, while delta Tth, Vent, Vent(exo-), Pfu, Ultma, BcaBEST, and KOD DNA polymerases did not.
Kenji Yamamoto +8 more
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A-Tailing with Taq Polymerase v3
, 2020This protocol can be used to add As to the blunt-ends of DNA fragments that have been amplified using a high-fidelity polymerase (such as Q5® High Fidelity DNA Polymerase).
New England Biolabs
semanticscholar +1 more source
Magnetic silica nanoparticle–Taq polymerase hybrids for multiple uses in polymerase chain reaction
, 2015Enzyme-magnetic nanoparticle hybrids have been employed as a strategy to improve the biocatalytic usability of biological molecules. For this purpose, we synthesized magnetic core–silica shell nanoparticles for direct one-step easy immobilization of Taq ...
V. Ozalp, G. Bayramoglu, M. Arica
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