Bst polymerase — a humble relative of Taq polymerase [PDF]
Computational and Structural Biotechnology Journal, 2023 DNA polymerases are a superfamily of enzymes synthesizing DNA using DNA as a template. They are essential for nucleic acid metabolism and for DNA replication and repair.
Igor Oscorbin, Maxim Filipenko
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Cost-effective in-house COVID-19 reverse transcription-polymerase chain reaction testing with yeast-derived Taq polymerase [PDF]
Annals of Thoracic MedicineBACKGROUND: Despite the decline of the COVID-19 pandemic, there continues to be a persistent requirement for reliable testing methods that can be adapted to future outbreaks and areas with limited resources. While the standard approach of using reverse
Mahmoud Zhra +6 more
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Live culture-based qPCR screening of Taq DNA polymerase variants for resistance to PCR inhibitors [PDF]
Frontiers in Bioengineering and BiotechnologyWe present a live culture PCR (LC-PCR) workflow that enables direct screening of randomly mutagenized Thermus aquaticus (Taq) and Klentaq1 DNA polymerase libraries without enzyme purification.
Milko B. Kermekchiev +3 more
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Mutant Taq DNA polymerases with improved elongation ability as a useful reagent for genetic engineering [PDF]
Frontiers in Microbiology, 2014 DNA polymerases are widely used for DNA manipulation in vitro, including DNA cloning, sequencing, DNA labeling, mutagenesis, and other experiments. Thermostable DNA polymerases are especially useful and became quite valuable after the development of PCR ...
Takeshi eYamagami +4 more
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Clamping-mediated incorporation of single-stranded DNA with concomitant DNA synthesis by Taq polymerase involves nick-translation [PDF]
Scientific ReportsThe development and characterization of a new enzyme reaction contribute to advancements in modern biotechnology. Here, we report a novel CIS (clamping-mediated incorporation of single-stranded DNA with concomitant DNA synthesis) reaction catalyzed by ...
Yoshiyuki Ohtsubo +2 more
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A-Tailing with Taq Polymerase v2
, 2020 This protocol can be used to add As to the blunt-ends of DNA fragments that have been amplified using a high-fidelity polymerase (such as Q5® High Fidelity DNA Polymerase).
New England Biolabs
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مجله دانشکده پزشکی اصفهان, 2012 Background: The aim of this project was to isolate and purify the highly active recombinant Taq DNA polymerase from the strain of Escherichia coli BL21.
Hamid Mirmohammad Sadeghi +3 more
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Optimizing Taq polymerase concentration for improved signal-to-noise in the broad range detection of low abundance bacteria. [PDF]
PLoS ONE, 2009 BACKGROUND:PCR in principle can detect a single target molecule in a reaction mixture. Contaminating bacterial DNA in reagents creates a practical limit on the use of PCR to detect dilute bacterial DNA in environmental or public health samples.
Rudolph Spangler +2 more
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Heat-Mediated Activation of Affinity-Immobilized Taq DNA Polymerase [PDF]
BioTechniques, 1997 A novel strategy for heat-mediated activation of recombinant Taq DNA polymerase is described. A serum albumin binding protein tag is used to affinity-immobilize an E. coli-expressed Taq DNA polymerase fusion protein onto a solid support coated with human
J. Nilsson +5 more
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A simplified protocol for producing Taq DNA polymerase in biology laboratory [PDF]
Research in Molecular Medicine, 2013 Background: Taq DNA polymerase is a very important enzyme for molecular biological studies such as DNA amplification and DNA sequencing by the PCR. It is a standard enzyme that is used in 90% of molecular biology labs today.
Touraj Farazmandfar +5 more
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