Results 11 to 20 of about 819,635 (326)

dUTPs conjugated with zwitterionic Cy3 or Cy5 fluorophore analogues are effective substrates for DNA amplification and labelling by Taq polymerase. [PDF]

Nucleic Acids Res, 2018
To develop structural modifications of dNTPs that are compatible with Taq DNA polymerase activity, we synthesized eight dUTP derivatives conjugated with Cy3 or Cy5 dye analogues that differed in charge and charge distribution throughout the fluorophore ...
Zasedateleva OA, Vasiliskov VA, Surzhikov SA, Kuznetsova VE, Shershov VE, Guseinov TO, Smirnov IP, Yurasov RA, Spitsyn MA, Chudinov AV.   +9 more
europepmc   +4 more sources

Cost-effective in-house COVID-19 reverse transcription-polymerase chain reaction testing with yeast-derived Taq polymerase [PDF]

Annals of Thoracic Medicine
BACKGROUND: Despite the decline of the COVID-19 pandemic, there continues to be a persistent requirement for reliable testing methods that can be adapted to future outbreaks and areas with limited resources. While the standard approach of using reverse
Mahmoud Zhra, Aljohara Al Saud, Maha Alzayer, Liliane Okdah, Hani Tamim, Hana M. A Fakhoury, Ahmad Aljada   +6 more
doaj   +3 more sources

Enhancing the reverse transcriptase function in Taq polymerase via AI-driven multiparametric rational design [PDF]

Frontiers in Bioengineering and Biotechnology
IntroductionModification of natural enzymes to introduce new properties and enhance existing ones is a central challenge in bioengineering. This study is focused on the development of Taq polymerase mutants that show enhanced reverse transcriptase (RTase)
Yulia E. Tomilova, Nikolay E. Russkikh, Igor M. Yi, Elizaveta V. Shaburova, Viktor N. Tomilov, Galina B. Pyrinova, Svetlana O. Brezhneva, Olga S. Tikhonyuk, Nadezhda S. Gololobova, Dmitriy V. Popichenko, Maxim O. Arkhipov, Leonid O. Bryzgalov, Evgeniy V. Brenner, Anastasia A. Artyukh, Dmitry N. Shtokalo, Dmitry N. Shtokalo, Dmitry N. Shtokalo, Denis V. Antonets, Mikhail K. Ivanov, Mikhail K. Ivanov   +19 more
doaj   +4 more sources

A simple and efficient method for extraction of Taq DNA polymerase

Electronic Journal of Biotechnology, 2015
AbstractBackgroundThermostable DNA polymerase (Taq Pol Ι) from Thermus aquaticus has been widely used in PCR, which was usually extracted with Pluthero's method. The method used ammonium sulfate to precipitate the enzyme, and it saved effort and money but not time.
Chen, Sique, Zheng, Xiujuan, Cao, Hongrui, Jiang, Linghui, Liu, Fangqian, Sun, Xinli   +5 more
doaj   +6 more sources

Switching the activity of Taq polymerase using clamp-like triplex aptamer structure. [PDF]

Nucleic Acids Res, 2020
In nature, allostery is the principal approach for regulating cellular processes and pathways. Inspired by nature, structure-switching aptamer-based nanodevices are widely used in artificial biotechnologies.
Hu Y, Wang Z, Chen Z, Pan L.
europepmc   +2 more sources

A Single Amino Acid Change to Taq DNA Polymerase Enables Faster PCR, Reverse Transcription and Strand-Displacement

Frontiers in Bioengineering and Biotechnology, 2021
A change of an aspartic acid to asparagine of Taq (Thermus aquaticus) DNA polymerase is a gain of function mutation that supports faster PCR: the extension times for PCR amplification can be 2–3 times shorter.
Wayne M. Barnes, Wayne M. Barnes, Zhian Zhang, Milko B. Kermekchiev   +3 more
doaj   +2 more sources

Highly multiplex PCR assays by coupling the 5′-flap endonuclease activity of Taq DNA polymerase and molecular beacon reporters

Proceedings of the National Academy of Sciences of the United States of America, 2022
Significance We describe a highly multiplex PCR approach that can identify 10-fold more targets in current real-time PCR assays without additional enzymes or separate reactions.
Qiuying Huang, Dongmei Chen, Chen Du, Qiaoqiao Liu, Su Lin, Lanlan Liang, Ye Xu, Yiqun Liao, Qingge Li   +8 more
openalex   +2 more sources

Obstacles of Multiplex Real-Time PCR for Bacterial 16S rDNA: Primer Specifity and DNA Decontamination of Taq Polymerase. [PDF]

Transfus Med Hemother, 2010
Philipp S, Huemer HP, Irschick EU, Gassner C.   +3 more
europepmc   +4 more sources

Structure-specific DNA-induced Conformational Changes in Taq Polymerase Revealed by Small Angle Neutron Scattering [PDF]

Journal of Biological Chemistry, 2004
The DNA polymerase I from Thermus aquaticus (Taq polymerase) performs lagging-strand DNA synthesis and DNA repair. Taq polymerase contains a polymerase domain for synthesizing a new DNA strand and a 5′-nuclease domain for cleaving RNA primers or damaged ...
Derek Ho, W. Malcolm Byrnes, Wu‐Po Ma, Yuan Shi, David J.E. Callaway, Zimei Bu   +5 more
openalex   +2 more sources

Clamping-mediated incorporation of single-stranded DNA with concomitant DNA synthesis by Taq polymerase involves nick-translation [PDF]

Scientific Reports
The development and characterization of a new enzyme reaction contribute to advancements in modern biotechnology. Here, we report a novel CIS (clamping-mediated incorporation of single-stranded DNA with concomitant DNA synthesis) reaction catalyzed by ...
Yoshiyuki Ohtsubo, Syoutaro Kawahara, Yuji Nagata   +2 more
doaj   +2 more sources