Results 11 to 20 of about 666,195 (194)
Structure-specific DNA-induced Conformational Changes in Taq Polymerase Revealed by Small Angle Neutron Scattering [PDF]
Journal of Biological Chemistry, 2004 The DNA polymerase I from Thermus aquaticus (Taq polymerase) performs lagging-strand DNA synthesis and DNA repair. Taq polymerase contains a polymerase domain for synthesizing a new DNA strand and a 5′-nuclease domain for cleaving RNA primers or damaged ...
Derek Ho +5 more
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Data of expression and purification of recombinant Taq DNA polymerase
Data in Brief, 2016 Polymerase chain reaction (PCR) technique is widely used in many experimental conditions, and Taq DNA polymerase is critical in PCR process. In this article, the Taq DNA polymerase expression plasmid is reconstructed and the protein product is obtained ...
Na Fang +4 more
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DNA sequencing using Taq polymerase
Nucleic Acids Research, 1988 Three DNA polymerases, namely E.coli DNA polymerase 1 (Klenow), reverse transcriptase and T7 DNA polymerase (sequenase), are commonly used for DNA sequencing by the chain termination method of Sanger and colleagues [1).
M. Peterson
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PLoS ONE, 2017 DNA polymerases are present in all organisms and are important enzymes that synthesise DNA molecules. They are used in various fields of science, predominantly as essential components for in vitro DNA syntheses, known as PCR.
Marcin Olszewski +3 more
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Reverse transcriptase inhibits Taq polymerase activity
Nucleic Acids Research, 1992 Detection of viral RNA by polymerase chain reaction (PCR) requires the prior reverse transcription of the viral RNA. In order to minimise the number of manual manipulations required for processing large numbers of samples, we attempted to design a system whereby all the reagents required for both reverse transcription and amplification can be added to ...
Robert Coelen +2 more
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Coupled protein domain motion inTaqpolymerase revealed by neutron spin-echo spectroscopy
Proceedings of the National Academy of Sciences of the United States of America, 2005 Long-range conformational changes in proteins are ubiquitous in biology for the transmission and amplification of signals; such conformational changes can be triggered by small-amplitude, nanosecond protein domain motion. Understanding how conformational
Zimei Bu +4 more
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A-Tailing with Taq Polymerase v1
, 2015 This protocol can be used to add As to the blunt-ends of DNA fragments that have been amplified using a high-fidelity polymerase (such as Q5® High Fidelity DNA Polymerase).
N. Biolabs
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Trehalose Is a Potent PCR Enhancer: Lowering of DNA Melting Temperature and Thermal Stabilization of Taq Polymerase by the Disaccharide Trehalose [PDF]
Clinical Chemistry, 2004 Compatible solutes are a class of compounds that stabilize cells and cellular components exposed to extreme conditions. In bacterial systems, the uptake or synthesis of compatible solutes renders the cells and their enzymatic machinery more resistant to ...
Andrej‐Nikolai Spiess +2 more
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Extreme free energy of stabilization of Taq DNA polymerase
Proteins: Structure, Function, and Bioinformatics, 2004 AbstractWe have examined the chemical denaturations of the Klentaq and Klenow large‐fragment domains of the Type 1 DNA polymerases from Thermus aquaticus (Klentaq) and Escherichia coli (Klenow) under identical solution conditions in order to directly compare the stabilization energetics of the two proteins.
Allyn J. Schoeffler +5 more
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Annals of Thoracic MedicineBACKGROUND: Despite the decline of the COVID-19 pandemic, there continues to be a persistent requirement for reliable testing methods that can be adapted to future outbreaks and areas with limited resources. While the standard approach of using reverse
Mahmoud Zhra +6 more
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